Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1500 and Pf1N1B4_1501 are separated by 470 nucleotides Pf1N1B4_1501 and Pf1N1B4_1502 overlap by 25 nucleotides Pf1N1B4_1502 and Pf1N1B4_1503 overlap by 20 nucleotides
Pf1N1B4_1500 - Chromosome (plasmid) partitioning protein ParA, at 1,734,188 to 1,734,958
_1500
Pf1N1B4_1501 - 3-oxoacyl-[ACP] synthase, at 1,735,429 to 1,736,043
_1501
Pf1N1B4_1502 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,736,019 to 1,736,828
_1502
Pf1N1B4_1503 - Acyl carrier protein (ACP1), at 1,736,809 to 1,737,069
_1503
Position (kb)
1735
1736
1737 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1734.576 kb on + strand, within Pf1N1B4_1500 at 1734.576 kb on + strand, within Pf1N1B4_1500 at 1734.588 kb on + strand, within Pf1N1B4_1500 at 1734.717 kb on + strand, within Pf1N1B4_1500 at 1734.717 kb on + strand, within Pf1N1B4_1500 at 1734.717 kb on + strand, within Pf1N1B4_1500 at 1734.718 kb on - strand, within Pf1N1B4_1500 at 1734.718 kb on - strand, within Pf1N1B4_1500 at 1734.822 kb on + strand, within Pf1N1B4_1500 at 1734.975 kb on - strand at 1734.994 kb on + strand at 1734.994 kb on + strand at 1734.994 kb on + strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.998 kb on - strand at 1734.998 kb on - strand at 1734.998 kb on - strand at 1735.014 kb on + strand at 1735.015 kb on - strand at 1735.015 kb on - strand at 1735.015 kb on - strand at 1735.019 kb on - strand at 1735.019 kb on - strand at 1735.019 kb on - strand at 1735.019 kb on - strand at 1735.039 kb on - strand at 1735.053 kb on - strand at 1735.053 kb on - strand at 1735.189 kb on - strand at 1735.255 kb on - strand at 1735.255 kb on - strand at 1735.255 kb on - strand at 1735.255 kb on - strand at 1735.541 kb on + strand, within Pf1N1B4_1501 at 1735.541 kb on + strand, within Pf1N1B4_1501 at 1735.554 kb on + strand, within Pf1N1B4_1501 at 1735.554 kb on + strand, within Pf1N1B4_1501 at 1735.554 kb on + strand, within Pf1N1B4_1501 at 1735.555 kb on - strand, within Pf1N1B4_1501 at 1735.555 kb on - strand, within Pf1N1B4_1501 at 1735.748 kb on + strand, within Pf1N1B4_1501 at 1735.748 kb on + strand, within Pf1N1B4_1501 at 1735.749 kb on - strand, within Pf1N1B4_1501 at 1735.749 kb on - strand, within Pf1N1B4_1501 at 1735.749 kb on - strand, within Pf1N1B4_1501 at 1735.787 kb on + strand, within Pf1N1B4_1501 at 1735.797 kb on - strand, within Pf1N1B4_1501 at 1735.826 kb on - strand, within Pf1N1B4_1501 at 1735.826 kb on - strand, within Pf1N1B4_1501 at 1735.969 kb on - strand, within Pf1N1B4_1501 at 1736.018 kb on + strand at 1736.019 kb on - strand at 1736.019 kb on - strand at 1736.068 kb on - strand at 1736.122 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.462 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.772 kb on + strand at 1736.772 kb on + strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.789 kb on + strand at 1736.789 kb on + strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.793 kb on - strand at 1736.972 kb on - strand, within Pf1N1B4_1503 at 1737.010 kb on - strand, within Pf1N1B4_1503
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,734,576 + Pf1N1B4_1500 0.50 -2.2 1,734,576 + Pf1N1B4_1500 0.50 -0.0 1,734,588 + Pf1N1B4_1500 0.52 -1.9 1,734,717 + Pf1N1B4_1500 0.69 -0.0 1,734,717 + Pf1N1B4_1500 0.69 -0.0 1,734,717 + Pf1N1B4_1500 0.69 -0.0 1,734,718 - Pf1N1B4_1500 0.69 -0.0 1,734,718 - Pf1N1B4_1500 0.69 -1.4 1,734,822 + Pf1N1B4_1500 0.82 -0.0 1,734,975 - -0.0 1,734,994 + -1.7 1,734,994 + -0.0 1,734,994 + -1.7 1,734,995 - -1.9 1,734,995 - -0.7 1,734,995 - -0.0 1,734,995 - -0.0 1,734,995 - -0.0 1,734,995 - -0.0 1,734,995 - -0.0 1,734,995 - -0.0 1,734,998 - -3.3 1,734,998 - -3.5 1,734,998 - -0.8 1,735,014 + -0.0 1,735,015 - -0.0 1,735,015 - -1.2 1,735,015 - -1.5 1,735,019 - -0.0 1,735,019 - -0.0 1,735,019 - -0.8 1,735,019 - -0.0 1,735,039 - -0.0 1,735,053 - -0.0 1,735,053 - -1.3 1,735,189 - -2.5 1,735,255 - -0.0 1,735,255 - -0.0 1,735,255 - +0.7 1,735,255 - -0.0 1,735,541 + Pf1N1B4_1501 0.18 -3.0 1,735,541 + Pf1N1B4_1501 0.18 -0.0 1,735,554 + Pf1N1B4_1501 0.20 -0.0 1,735,554 + Pf1N1B4_1501 0.20 -4.1 1,735,554 + Pf1N1B4_1501 0.20 -0.0 1,735,555 - Pf1N1B4_1501 0.20 -0.0 1,735,555 - Pf1N1B4_1501 0.20 -0.0 1,735,748 + Pf1N1B4_1501 0.52 -0.0 1,735,748 + Pf1N1B4_1501 0.52 -0.0 1,735,749 - Pf1N1B4_1501 0.52 -4.0 1,735,749 - Pf1N1B4_1501 0.52 -0.0 1,735,749 - Pf1N1B4_1501 0.52 -0.0 1,735,787 + Pf1N1B4_1501 0.58 -1.6 1,735,797 - Pf1N1B4_1501 0.60 -2.6 1,735,826 - Pf1N1B4_1501 0.65 -1.8 1,735,826 - Pf1N1B4_1501 0.65 -2.0 1,735,969 - Pf1N1B4_1501 0.88 -0.0 1,736,018 + -3.6 1,736,019 - -0.0 1,736,019 - -2.3 1,736,068 - -0.9 1,736,122 + Pf1N1B4_1502 0.13 -1.5 1,736,262 + Pf1N1B4_1502 0.30 -0.0 1,736,262 + Pf1N1B4_1502 0.30 -0.0 1,736,262 + Pf1N1B4_1502 0.30 -0.0 1,736,263 - Pf1N1B4_1502 0.30 -0.0 1,736,263 - Pf1N1B4_1502 0.30 -0.0 1,736,263 - Pf1N1B4_1502 0.30 -0.9 1,736,263 - Pf1N1B4_1502 0.30 -0.0 1,736,263 - Pf1N1B4_1502 0.30 -0.0 1,736,263 - Pf1N1B4_1502 0.30 -0.0 1,736,437 - Pf1N1B4_1502 0.52 -0.0 1,736,437 - Pf1N1B4_1502 0.52 -2.6 1,736,437 - Pf1N1B4_1502 0.52 -1.0 1,736,437 - Pf1N1B4_1502 0.52 -0.0 1,736,437 - Pf1N1B4_1502 0.52 -2.8 1,736,462 + Pf1N1B4_1502 0.55 -0.0 1,736,710 + Pf1N1B4_1502 0.85 -0.0 1,736,710 + Pf1N1B4_1502 0.85 -1.5 1,736,710 + Pf1N1B4_1502 0.85 -0.0 1,736,711 - Pf1N1B4_1502 0.85 -1.7 1,736,711 - Pf1N1B4_1502 0.85 -4.8 1,736,711 - Pf1N1B4_1502 0.85 -2.9 1,736,711 - Pf1N1B4_1502 0.85 -1.6 1,736,711 - Pf1N1B4_1502 0.85 -0.0 1,736,711 - Pf1N1B4_1502 0.85 -0.0 1,736,711 - Pf1N1B4_1502 0.85 -0.0 1,736,711 - Pf1N1B4_1502 0.85 -0.0 1,736,772 + -0.0 1,736,772 + -0.0 1,736,773 - -0.0 1,736,773 - -0.0 1,736,773 - -1.9 1,736,773 - -0.0 1,736,784 + -0.0 1,736,784 + -0.0 1,736,784 + -1.6 1,736,784 + -0.0 1,736,785 - -0.0 1,736,785 - -1.6 1,736,785 - -0.0 1,736,785 - -0.0 1,736,785 - -0.0 1,736,785 - -0.0 1,736,785 - -2.6 1,736,785 - -0.0 1,736,789 + -4.4 1,736,789 + -2.0 1,736,790 - -0.0 1,736,790 - -1.6 1,736,790 - -1.0 1,736,790 - -0.0 1,736,793 - -1.5 1,736,972 - Pf1N1B4_1503 0.62 -3.0 1,737,010 - Pf1N1B4_1503 0.77 -0.0
Or see this region's nucleotide sequence