Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_148 and Pf1N1B4_149 overlap by 4 nucleotides Pf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotides
Pf1N1B4_148 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 182,592 to 188,378
_148
Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797
_149
Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085
_150
Position (kb) 188
189
190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 187.720 kb on + strand, within Pf1N1B4_148 at 188.378 kb on + strand at 188.379 kb on - strand at 188.379 kb on - strand at 188.691 kb on - strand at 188.691 kb on - strand at 188.886 kb on + strand, within Pf1N1B4_149 at 189.033 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand at 189.530 kb on - strand, within Pf1N1B4_149 at 189.783 kb on + strand, within Pf1N1B4_149 at 189.783 kb on + strand, within Pf1N1B4_149 at 189.784 kb on - strand, within Pf1N1B4_149 at 189.813 kb on - strand, within Pf1N1B4_149 at 190.002 kb on + strand, within Pf1N1B4_149 at 190.002 kb on + strand, within Pf1N1B4_149 at 190.003 kb on - strand, within Pf1N1B4_149 at 190.003 kb on - strand, within Pf1N1B4_149 at 190.150 kb on - strand, within Pf1N1B4_149 at 190.413 kb on + strand, within Pf1N1B4_149 at 190.413 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.584 kb on - strand at 191.584 kb on - strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.638 kb on - strand at 191.883 kb on + strand at 191.906 kb on - strand at 192.305 kb on - strand, within Pf1N1B4_150 at 192.598 kb on + strand at 192.653 kb on - strand, within Pf1N1B4_150 at 192.653 kb on - strand, within Pf1N1B4_150 at 192.712 kb on + strand, within Pf1N1B4_150 at 192.713 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 187,720 + Pf1N1B4_148 0.89 -1.5 188,378 + -2.5 188,379 - -0.0 188,379 - -0.0 188,691 - -2.0 188,691 - -1.9 188,886 + Pf1N1B4_149 0.15 -0.0 189,033 + Pf1N1B4_149 0.19 -0.0 189,180 + Pf1N1B4_149 0.24 -0.0 189,180 + Pf1N1B4_149 0.24 -0.0 189,180 + -0.0 189,530 - Pf1N1B4_149 0.34 -0.0 189,783 + Pf1N1B4_149 0.41 -0.0 189,783 + Pf1N1B4_149 0.41 -0.0 189,784 - Pf1N1B4_149 0.41 -0.0 189,813 - Pf1N1B4_149 0.42 -0.0 190,002 + Pf1N1B4_149 0.48 -0.0 190,002 + Pf1N1B4_149 0.48 -0.0 190,003 - Pf1N1B4_149 0.48 -0.0 190,003 - Pf1N1B4_149 0.48 -0.0 190,150 - Pf1N1B4_149 0.52 -0.0 190,413 + Pf1N1B4_149 0.60 -2.4 190,413 + Pf1N1B4_149 0.60 -0.0 191,193 + Pf1N1B4_149 0.82 -0.0 191,193 + Pf1N1B4_149 0.82 -2.6 191,194 - Pf1N1B4_149 0.82 -0.0 191,194 - Pf1N1B4_149 0.82 -2.8 191,584 - -2.0 191,584 - -0.0 191,637 + -0.0 191,637 + -0.0 191,637 + -0.0 191,638 - -0.0 191,883 + -3.6 191,906 - -0.0 192,305 - Pf1N1B4_150 0.21 -1.2 192,598 + +0.3 192,653 - Pf1N1B4_150 0.56 -0.0 192,653 - Pf1N1B4_150 0.56 -0.0 192,712 + Pf1N1B4_150 0.62 -0.0 192,713 - Pf1N1B4_150 0.62 -2.4 192,746 - Pf1N1B4_150 0.65 -0.0 192,746 - Pf1N1B4_150 0.65 -1.4 192,746 - Pf1N1B4_150 0.65 -0.1
Or see this region's nucleotide sequence