Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_140 and Pf1N1B4_141 are separated by 272 nucleotides Pf1N1B4_141 and Pf1N1B4_142 are separated by 210 nucleotides
Pf1N1B4_140 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 157,242 to 158,129
_140
Pf1N1B4_141 - PvdE, pyoverdine ABC export system, fused ATPase and permease components, at 158,402 to 160,051
_141
Pf1N1B4_142 - TonB-dependent siderophore receptor precursor, at 160,262 to 162,742
_142
Position (kb) 158
159
160
161 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 157.542 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.743 kb on + strand, within Pf1N1B4_140 at 157.744 kb on - strand at 157.831 kb on - strand, within Pf1N1B4_140 at 157.848 kb on + strand, within Pf1N1B4_140 at 157.921 kb on - strand, within Pf1N1B4_140 at 157.921 kb on - strand, within Pf1N1B4_140 at 157.953 kb on + strand, within Pf1N1B4_140 at 157.953 kb on + strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 158.113 kb on + strand at 158.215 kb on + strand at 158.230 kb on - strand at 158.230 kb on - strand at 158.314 kb on + strand at 158.315 kb on - strand at 158.335 kb on + strand at 158.336 kb on - strand at 158.365 kb on + strand at 158.645 kb on + strand, within Pf1N1B4_141 at 158.645 kb on + strand, within Pf1N1B4_141 at 158.645 kb on + strand, within Pf1N1B4_141 at 158.645 kb on + strand, within Pf1N1B4_141 at 158.646 kb on - strand, within Pf1N1B4_141 at 158.769 kb on + strand, within Pf1N1B4_141 at 158.770 kb on - strand, within Pf1N1B4_141 at 158.770 kb on - strand, within Pf1N1B4_141 at 158.878 kb on - strand, within Pf1N1B4_141 at 158.878 kb on - strand, within Pf1N1B4_141 at 159.116 kb on - strand, within Pf1N1B4_141 at 159.116 kb on - strand, within Pf1N1B4_141 at 159.245 kb on + strand, within Pf1N1B4_141 at 159.245 kb on + strand, within Pf1N1B4_141 at 159.245 kb on + strand, within Pf1N1B4_141 at 159.246 kb on - strand, within Pf1N1B4_141 at 159.384 kb on - strand, within Pf1N1B4_141 at 159.569 kb on + strand, within Pf1N1B4_141 at 159.570 kb on - strand, within Pf1N1B4_141 at 159.668 kb on + strand, within Pf1N1B4_141 at 159.669 kb on - strand, within Pf1N1B4_141 at 159.669 kb on - strand, within Pf1N1B4_141 at 159.669 kb on - strand, within Pf1N1B4_141 at 159.671 kb on + strand, within Pf1N1B4_141 at 159.672 kb on - strand, within Pf1N1B4_141 at 159.729 kb on - strand, within Pf1N1B4_141 at 160.068 kb on - strand at 160.145 kb on - strand at 160.145 kb on - strand at 160.145 kb on - strand at 160.145 kb on - strand at 160.239 kb on - strand at 160.382 kb on + strand at 160.382 kb on + strand at 160.382 kb on + strand at 160.382 kb on + strand at 160.478 kb on + strand at 160.478 kb on + strand at 160.479 kb on - strand at 160.479 kb on - strand at 160.479 kb on - strand at 160.479 kb on - strand at 160.851 kb on - strand, within Pf1N1B4_142 at 160.874 kb on + strand, within Pf1N1B4_142 at 160.874 kb on + strand, within Pf1N1B4_142 at 160.949 kb on + strand, within Pf1N1B4_142 at 160.949 kb on + strand, within Pf1N1B4_142 at 160.950 kb on - strand, within Pf1N1B4_142 at 160.989 kb on - strand, within Pf1N1B4_142 at 160.989 kb on - strand, within Pf1N1B4_142 at 160.989 kb on - strand, within Pf1N1B4_142
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 157,542 + Pf1N1B4_140 0.34 -0.0 157,677 + Pf1N1B4_140 0.49 -1.4 157,677 + Pf1N1B4_140 0.49 -0.0 157,677 + Pf1N1B4_140 0.49 -0.0 157,677 + Pf1N1B4_140 0.49 -0.0 157,743 + Pf1N1B4_140 0.56 -0.0 157,744 - -1.5 157,831 - Pf1N1B4_140 0.66 -0.0 157,848 + Pf1N1B4_140 0.68 -0.0 157,921 - Pf1N1B4_140 0.76 -0.0 157,921 - Pf1N1B4_140 0.76 -0.0 157,953 + Pf1N1B4_140 0.80 -3.6 157,953 + Pf1N1B4_140 0.80 -0.7 157,954 - Pf1N1B4_140 0.80 -0.0 157,954 - Pf1N1B4_140 0.80 -1.5 157,954 - Pf1N1B4_140 0.80 -0.0 157,954 - Pf1N1B4_140 0.80 -1.2 158,113 + -0.0 158,215 + -0.0 158,230 - -0.0 158,230 - -0.0 158,314 + -1.9 158,315 - -0.0 158,335 + -0.3 158,336 - -0.0 158,365 + -0.0 158,645 + Pf1N1B4_141 0.15 -0.0 158,645 + Pf1N1B4_141 0.15 -1.9 158,645 + Pf1N1B4_141 0.15 -0.0 158,645 + Pf1N1B4_141 0.15 -0.0 158,646 - Pf1N1B4_141 0.15 -0.0 158,769 + Pf1N1B4_141 0.22 -2.2 158,770 - Pf1N1B4_141 0.22 -0.0 158,770 - Pf1N1B4_141 0.22 -3.2 158,878 - Pf1N1B4_141 0.29 -0.0 158,878 - Pf1N1B4_141 0.29 -2.4 159,116 - Pf1N1B4_141 0.43 -1.2 159,116 - Pf1N1B4_141 0.43 -2.1 159,245 + Pf1N1B4_141 0.51 -0.0 159,245 + Pf1N1B4_141 0.51 -0.0 159,245 + Pf1N1B4_141 0.51 -4.0 159,246 - Pf1N1B4_141 0.51 -0.0 159,384 - Pf1N1B4_141 0.60 -1.8 159,569 + Pf1N1B4_141 0.71 -3.0 159,570 - Pf1N1B4_141 0.71 -0.0 159,668 + Pf1N1B4_141 0.77 -1.9 159,669 - Pf1N1B4_141 0.77 -0.0 159,669 - Pf1N1B4_141 0.77 -4.2 159,669 - Pf1N1B4_141 0.77 -0.0 159,671 + Pf1N1B4_141 0.77 -3.2 159,672 - Pf1N1B4_141 0.77 -0.0 159,729 - Pf1N1B4_141 0.80 -2.6 160,068 - -0.0 160,145 - -0.0 160,145 - -2.4 160,145 - -3.5 160,145 - -1.1 160,239 - -0.0 160,382 + -0.0 160,382 + -1.6 160,382 + -1.3 160,382 + -0.0 160,478 + -0.0 160,478 + -1.5 160,479 - -0.0 160,479 - -0.0 160,479 - -0.0 160,479 - -3.2 160,851 - Pf1N1B4_142 0.24 -2.2 160,874 + Pf1N1B4_142 0.25 -0.0 160,874 + Pf1N1B4_142 0.25 -2.7 160,949 + Pf1N1B4_142 0.28 -0.0 160,949 + Pf1N1B4_142 0.28 -0.0 160,950 - Pf1N1B4_142 0.28 -0.8 160,989 - Pf1N1B4_142 0.29 -1.8 160,989 - Pf1N1B4_142 0.29 -2.2 160,989 - Pf1N1B4_142 0.29 -0.0
Or see this region's nucleotide sequence