Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1387 and Pf1N1B4_1388 are separated by 192 nucleotides Pf1N1B4_1388 and Pf1N1B4_1389 are separated by 344 nucleotides Pf1N1B4_1389 and Pf1N1B4_1390 are separated by 68 nucleotides Pf1N1B4_1390 and Pf1N1B4_1391 are separated by 220 nucleotides
Pf1N1B4_1387 - NAD synthetase (EC 6.3.1.5), at 1,587,794 to 1,588,621
_1387
Pf1N1B4_1388 - Azurin, at 1,588,814 to 1,589,260
_1388
Pf1N1B4_1389 - Lysine decarboxylase family, at 1,589,605 to 1,590,192
_1389
Pf1N1B4_1390 - FIG00956548: hypothetical protein, at 1,590,261 to 1,590,449
_1390
Pf1N1B4_1391 - Protein containing transglutaminase-like domain, putative cysteine protease, at 1,590,670 to 1,591,560
_1391
Position (kb)
1589
1590
1591 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1588.755 kb on - strand at 1588.811 kb on + strand at 1588.879 kb on - strand, within Pf1N1B4_1388 at 1588.986 kb on + strand, within Pf1N1B4_1388 at 1588.986 kb on + strand, within Pf1N1B4_1388 at 1588.986 kb on + strand, within Pf1N1B4_1388 at 1589.062 kb on + strand, within Pf1N1B4_1388 at 1589.063 kb on - strand, within Pf1N1B4_1388 at 1589.370 kb on - strand at 1589.370 kb on - strand at 1589.391 kb on + strand at 1589.391 kb on + strand at 1589.392 kb on - strand at 1589.392 kb on - strand at 1589.418 kb on - strand at 1589.495 kb on + strand at 1589.543 kb on + strand at 1589.572 kb on + strand at 1589.572 kb on + strand at 1589.573 kb on - strand at 1589.573 kb on - strand at 1589.599 kb on + strand at 1589.599 kb on + strand at 1589.600 kb on - strand at 1589.600 kb on - strand at 1589.600 kb on - strand at 1589.613 kb on - strand at 1589.627 kb on + strand at 1589.652 kb on + strand at 1589.662 kb on + strand at 1589.913 kb on + strand, within Pf1N1B4_1389 at 1589.913 kb on + strand, within Pf1N1B4_1389 at 1589.914 kb on - strand, within Pf1N1B4_1389 at 1590.014 kb on + strand, within Pf1N1B4_1389 at 1590.014 kb on + strand, within Pf1N1B4_1389 at 1590.015 kb on - strand, within Pf1N1B4_1389 at 1590.055 kb on - strand, within Pf1N1B4_1389 at 1590.055 kb on - strand, within Pf1N1B4_1389 at 1590.267 kb on - strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.272 kb on + strand at 1590.273 kb on - strand at 1590.273 kb on - strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.276 kb on + strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.277 kb on - strand at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.350 kb on + strand, within Pf1N1B4_1390 at 1590.351 kb on - strand, within Pf1N1B4_1390 at 1590.685 kb on + strand at 1590.686 kb on - strand at 1590.686 kb on - strand at 1590.686 kb on - strand at 1590.935 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.175 kb on + strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391 at 1591.176 kb on - strand, within Pf1N1B4_1391
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,588,755 - -0.0 1,588,811 + -0.0 1,588,879 - Pf1N1B4_1388 0.15 -0.0 1,588,986 + Pf1N1B4_1388 0.38 -0.0 1,588,986 + Pf1N1B4_1388 0.38 -0.0 1,588,986 + Pf1N1B4_1388 0.38 -2.4 1,589,062 + Pf1N1B4_1388 0.55 -3.3 1,589,063 - Pf1N1B4_1388 0.56 -0.0 1,589,370 - -1.4 1,589,370 - -0.0 1,589,391 + -0.0 1,589,391 + -0.0 1,589,392 - -0.0 1,589,392 - -0.0 1,589,418 - -1.6 1,589,495 + -0.0 1,589,543 + -0.0 1,589,572 + -0.0 1,589,572 + -0.0 1,589,573 - -0.0 1,589,573 - -2.5 1,589,599 + -0.6 1,589,599 + -0.0 1,589,600 - -0.0 1,589,600 - -0.0 1,589,600 - -0.0 1,589,613 - -2.1 1,589,627 + -1.8 1,589,652 + -1.7 1,589,662 + -1.2 1,589,913 + Pf1N1B4_1389 0.52 -0.0 1,589,913 + Pf1N1B4_1389 0.52 -0.0 1,589,914 - Pf1N1B4_1389 0.53 -0.0 1,590,014 + Pf1N1B4_1389 0.70 -0.0 1,590,014 + Pf1N1B4_1389 0.70 -0.0 1,590,015 - Pf1N1B4_1389 0.70 -0.0 1,590,055 - Pf1N1B4_1389 0.77 -0.0 1,590,055 - Pf1N1B4_1389 0.77 -3.7 1,590,267 - -0.8 1,590,272 + -2.4 1,590,272 + -1.6 1,590,272 + -1.0 1,590,272 + -0.0 1,590,272 + -0.0 1,590,273 - -0.0 1,590,273 - -0.0 1,590,276 + -1.8 1,590,276 + -0.0 1,590,276 + -0.0 1,590,276 + -0.0 1,590,276 + -2.6 1,590,277 - -0.0 1,590,277 - -1.9 1,590,277 - -1.2 1,590,277 - -0.0 1,590,350 + Pf1N1B4_1390 0.47 -1.7 1,590,350 + Pf1N1B4_1390 0.47 -1.9 1,590,350 + Pf1N1B4_1390 0.47 -0.0 1,590,350 + Pf1N1B4_1390 0.47 -3.3 1,590,350 + Pf1N1B4_1390 0.47 -0.0 1,590,350 + Pf1N1B4_1390 0.47 -0.0 1,590,350 + Pf1N1B4_1390 0.47 -1.2 1,590,351 - Pf1N1B4_1390 0.48 -0.0 1,590,685 + -0.0 1,590,686 - -1.1 1,590,686 - -0.0 1,590,686 - -2.3 1,590,935 + Pf1N1B4_1391 0.30 -1.3 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,175 + Pf1N1B4_1391 0.57 -1.7 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,175 + Pf1N1B4_1391 0.57 -0.0 1,591,176 - Pf1N1B4_1391 0.57 -0.0 1,591,176 - Pf1N1B4_1391 0.57 -3.1 1,591,176 - Pf1N1B4_1391 0.57 -0.0 1,591,176 - Pf1N1B4_1391 0.57 -2.1 1,591,176 - Pf1N1B4_1391 0.57 -2.1
Or see this region's nucleotide sequence