Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1346 and Pf1N1B4_1347 are separated by 168 nucleotides Pf1N1B4_1347 and Pf1N1B4_1348 are separated by 31 nucleotides Pf1N1B4_1348 and Pf1N1B4_1349 are separated by 158 nucleotides Pf1N1B4_1349 and Pf1N1B4_1350 are separated by 9 nucleotides
Pf1N1B4_1346 - Urea ABC transporter, ATPase protein UrtD, at 1,543,326 to 1,544,195
_1346
Pf1N1B4_1347 - Urea ABC transporter, ATPase protein UrtE, at 1,544,364 to 1,545,062
_1347
Pf1N1B4_1348 - Urease accessory protein UreD, at 1,545,094 to 1,545,933
_1348
Pf1N1B4_1349 - Urease gamma subunit (EC 3.5.1.5), at 1,546,092 to 1,546,394
_1349
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Position (kb) 1545
1546 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1544.181 kb on + strand at 1544.181 kb on + strand at 1544.395 kb on - strand at 1544.584 kb on + strand, within Pf1N1B4_1347 at 1544.602 kb on - strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.944 kb on - strand, within Pf1N1B4_1347 at 1544.944 kb on - strand, within Pf1N1B4_1347 at 1544.974 kb on - strand, within Pf1N1B4_1347 at 1544.974 kb on - strand, within Pf1N1B4_1347 at 1545.024 kb on - strand at 1545.024 kb on - strand at 1545.060 kb on + strand at 1545.087 kb on - strand at 1545.087 kb on - strand at 1545.101 kb on + strand at 1545.101 kb on + strand at 1545.101 kb on + strand at 1545.102 kb on - strand at 1545.102 kb on - strand at 1545.429 kb on + strand, within Pf1N1B4_1348 at 1545.429 kb on + strand, within Pf1N1B4_1348 at 1545.429 kb on + strand, within Pf1N1B4_1348 at 1545.459 kb on + strand, within Pf1N1B4_1348 at 1545.459 kb on + strand, within Pf1N1B4_1348 at 1545.776 kb on + strand, within Pf1N1B4_1348 at 1545.776 kb on + strand, within Pf1N1B4_1348 at 1545.776 kb on + strand, within Pf1N1B4_1348 at 1545.978 kb on - strand at 1545.978 kb on - strand at 1546.047 kb on - strand at 1546.067 kb on - strand at 1546.204 kb on - strand, within Pf1N1B4_1349 at 1546.410 kb on + strand at 1546.410 kb on + strand at 1546.411 kb on - strand at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.462 kb on - strand, within Pf1N1B4_1350 at 1546.598 kb on - strand, within Pf1N1B4_1350 at 1546.668 kb on + strand, within Pf1N1B4_1350 at 1546.669 kb on - strand, within Pf1N1B4_1350 at 1546.849 kb on + strand, within Pf1N1B4_1350
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,544,181 + +0.0 1,544,181 + -2.6 1,544,395 - +0.0 1,544,584 + Pf1N1B4_1347 0.31 +0.0 1,544,602 - Pf1N1B4_1347 0.34 +0.0 1,544,943 + Pf1N1B4_1347 0.83 -1.4 1,544,943 + Pf1N1B4_1347 0.83 +1.0 1,544,943 + Pf1N1B4_1347 0.83 +0.0 1,544,944 - Pf1N1B4_1347 0.83 +0.0 1,544,944 - Pf1N1B4_1347 0.83 +0.0 1,544,974 - Pf1N1B4_1347 0.87 +0.0 1,544,974 - Pf1N1B4_1347 0.87 -1.0 1,545,024 - +0.0 1,545,024 - +0.0 1,545,060 + -1.4 1,545,087 - +0.0 1,545,087 - +0.0 1,545,101 + +0.0 1,545,101 + +0.0 1,545,101 + +0.0 1,545,102 - -1.8 1,545,102 - +0.0 1,545,429 + Pf1N1B4_1348 0.40 +0.0 1,545,429 + Pf1N1B4_1348 0.40 +0.0 1,545,429 + Pf1N1B4_1348 0.40 -1.0 1,545,459 + Pf1N1B4_1348 0.43 +0.0 1,545,459 + Pf1N1B4_1348 0.43 -0.3 1,545,776 + Pf1N1B4_1348 0.81 +0.0 1,545,776 + Pf1N1B4_1348 0.81 +0.0 1,545,776 + Pf1N1B4_1348 0.81 +0.0 1,545,978 - -2.1 1,545,978 - +0.0 1,546,047 - -1.0 1,546,067 - +0.0 1,546,204 - Pf1N1B4_1349 0.37 +0.0 1,546,410 + -0.8 1,546,410 + +0.0 1,546,411 - -0.8 1,546,461 + Pf1N1B4_1350 0.11 +0.0 1,546,461 + Pf1N1B4_1350 0.11 -4.2 1,546,461 + Pf1N1B4_1350 0.11 +0.0 1,546,461 + Pf1N1B4_1350 0.11 -1.0 1,546,462 - Pf1N1B4_1350 0.11 +0.0 1,546,598 - Pf1N1B4_1350 0.36 +0.0 1,546,668 + Pf1N1B4_1350 0.49 +0.0 1,546,669 - Pf1N1B4_1350 0.50 +0.0 1,546,849 + Pf1N1B4_1350 0.83 -2.1
Or see this region's nucleotide sequence