Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1345 and Pf1N1B4_1346 overlap by 4 nucleotides Pf1N1B4_1346 and Pf1N1B4_1347 are separated by 168 nucleotides Pf1N1B4_1347 and Pf1N1B4_1348 are separated by 31 nucleotides
Pf1N1B4_1345 - Urea ABC transporter, permease protein UrtC, at 1,542,250 to 1,543,329
_1345
Pf1N1B4_1346 - Urea ABC transporter, ATPase protein UrtD, at 1,543,326 to 1,544,195
_1346
Pf1N1B4_1347 - Urea ABC transporter, ATPase protein UrtE, at 1,544,364 to 1,545,062
_1347
Pf1N1B4_1348 - Urease accessory protein UreD, at 1,545,094 to 1,545,933
_1348
Position (kb)
1543
1544
1545 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1542.333 kb on - strand at 1542.333 kb on - strand at 1542.333 kb on - strand at 1542.333 kb on - strand at 1542.395 kb on - strand, within Pf1N1B4_1345 at 1542.532 kb on + strand, within Pf1N1B4_1345 at 1542.532 kb on + strand, within Pf1N1B4_1345 at 1542.532 kb on + strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.533 kb on - strand, within Pf1N1B4_1345 at 1542.721 kb on + strand, within Pf1N1B4_1345 at 1542.721 kb on + strand, within Pf1N1B4_1345 at 1542.722 kb on - strand, within Pf1N1B4_1345 at 1542.722 kb on - strand, within Pf1N1B4_1345 at 1543.066 kb on + strand at 1543.066 kb on + strand, within Pf1N1B4_1345 at 1543.066 kb on + strand, within Pf1N1B4_1345 at 1543.067 kb on - strand, within Pf1N1B4_1345 at 1543.067 kb on - strand, within Pf1N1B4_1345 at 1543.087 kb on - strand, within Pf1N1B4_1345 at 1543.234 kb on + strand at 1543.279 kb on + strand at 1543.279 kb on + strand at 1543.279 kb on + strand at 1543.279 kb on + strand at 1543.280 kb on - strand at 1543.280 kb on - strand at 1543.280 kb on - strand at 1543.280 kb on - strand at 1543.287 kb on + strand at 1543.400 kb on + strand at 1543.533 kb on + strand, within Pf1N1B4_1346 at 1543.533 kb on + strand, within Pf1N1B4_1346 at 1543.534 kb on - strand, within Pf1N1B4_1346 at 1543.892 kb on + strand, within Pf1N1B4_1346 at 1543.892 kb on + strand, within Pf1N1B4_1346 at 1543.893 kb on - strand, within Pf1N1B4_1346 at 1543.893 kb on - strand, within Pf1N1B4_1346 at 1544.181 kb on + strand at 1544.181 kb on + strand at 1544.395 kb on - strand at 1544.584 kb on + strand, within Pf1N1B4_1347 at 1544.602 kb on - strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.943 kb on + strand, within Pf1N1B4_1347 at 1544.944 kb on - strand, within Pf1N1B4_1347 at 1544.944 kb on - strand, within Pf1N1B4_1347 at 1544.974 kb on - strand, within Pf1N1B4_1347 at 1544.974 kb on - strand, within Pf1N1B4_1347 at 1545.024 kb on - strand at 1545.024 kb on - strand at 1545.060 kb on + strand at 1545.087 kb on - strand at 1545.087 kb on - strand at 1545.101 kb on + strand at 1545.101 kb on + strand at 1545.101 kb on + strand at 1545.102 kb on - strand at 1545.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,542,333 - -2.3 1,542,333 - +0.0 1,542,333 - +0.0 1,542,333 - +0.0 1,542,395 - Pf1N1B4_1345 0.13 +0.0 1,542,532 + Pf1N1B4_1345 0.26 +0.0 1,542,532 + Pf1N1B4_1345 0.26 +0.0 1,542,532 + Pf1N1B4_1345 0.26 +0.0 1,542,533 - Pf1N1B4_1345 0.26 +0.0 1,542,533 - Pf1N1B4_1345 0.26 -2.4 1,542,533 - Pf1N1B4_1345 0.26 -3.8 1,542,533 - Pf1N1B4_1345 0.26 +0.0 1,542,721 + Pf1N1B4_1345 0.44 +0.0 1,542,721 + Pf1N1B4_1345 0.44 +0.0 1,542,722 - Pf1N1B4_1345 0.44 -1.5 1,542,722 - Pf1N1B4_1345 0.44 -0.6 1,543,066 + +0.0 1,543,066 + Pf1N1B4_1345 0.76 +0.0 1,543,066 + Pf1N1B4_1345 0.76 +0.0 1,543,067 - Pf1N1B4_1345 0.76 +0.0 1,543,067 - Pf1N1B4_1345 0.76 +0.0 1,543,087 - Pf1N1B4_1345 0.78 +0.0 1,543,234 + +0.0 1,543,279 + +0.0 1,543,279 + -2.1 1,543,279 + +0.0 1,543,279 + +0.0 1,543,280 - -1.7 1,543,280 - -3.9 1,543,280 - +0.0 1,543,280 - -3.3 1,543,287 + +0.0 1,543,400 + +0.0 1,543,533 + Pf1N1B4_1346 0.24 +0.0 1,543,533 + Pf1N1B4_1346 0.24 +0.0 1,543,534 - Pf1N1B4_1346 0.24 -4.0 1,543,892 + Pf1N1B4_1346 0.65 -2.4 1,543,892 + Pf1N1B4_1346 0.65 +0.0 1,543,893 - Pf1N1B4_1346 0.65 +0.0 1,543,893 - Pf1N1B4_1346 0.65 -2.8 1,544,181 + +0.0 1,544,181 + -2.6 1,544,395 - +0.0 1,544,584 + Pf1N1B4_1347 0.31 +0.0 1,544,602 - Pf1N1B4_1347 0.34 +0.0 1,544,943 + Pf1N1B4_1347 0.83 -1.4 1,544,943 + Pf1N1B4_1347 0.83 +1.0 1,544,943 + Pf1N1B4_1347 0.83 +0.0 1,544,944 - Pf1N1B4_1347 0.83 +0.0 1,544,944 - Pf1N1B4_1347 0.83 +0.0 1,544,974 - Pf1N1B4_1347 0.87 +0.0 1,544,974 - Pf1N1B4_1347 0.87 -1.0 1,545,024 - +0.0 1,545,024 - +0.0 1,545,060 + -1.4 1,545,087 - +0.0 1,545,087 - +0.0 1,545,101 + +0.0 1,545,101 + +0.0 1,545,101 + +0.0 1,545,102 - -1.8 1,545,102 - +0.0
Or see this region's nucleotide sequence