Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1338 and Pf1N1B4_1339 are separated by 540 nucleotides Pf1N1B4_1339 and Pf1N1B4_1340 overlap by 4 nucleotides Pf1N1B4_1340 and Pf1N1B4_1341 overlap by 4 nucleotides
Pf1N1B4_1338 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 1,533,225 to 1,534,601
_1338
Pf1N1B4_1339 - ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component, at 1,535,142 to 1,536,113
_1339
Pf1N1B4_1340 - ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component, at 1,536,110 to 1,537,057
_1340
Pf1N1B4_1341 - ABC transporter (iron.B12.siderophore.hemin) , permease component, at 1,537,054 to 1,538,064
_1341
Position (kb) 1535
1536
1537 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1534.263 kb on + strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.264 kb on - strand, within Pf1N1B4_1338 at 1534.572 kb on - strand at 1534.572 kb on - strand at 1534.645 kb on - strand at 1534.826 kb on + strand at 1534.921 kb on + strand at 1534.921 kb on + strand at 1534.922 kb on - strand at 1534.922 kb on - strand at 1534.922 kb on - strand at 1534.961 kb on - strand at 1534.961 kb on - strand at 1534.961 kb on - strand at 1534.984 kb on + strand at 1534.985 kb on - strand at 1534.985 kb on - strand at 1535.025 kb on + strand at 1535.026 kb on - strand at 1535.032 kb on + strand at 1535.032 kb on + strand at 1535.032 kb on + strand at 1535.032 kb on + strand at 1535.130 kb on - strand at 1535.139 kb on - strand at 1535.139 kb on - strand at 1535.139 kb on - strand at 1535.536 kb on - strand, within Pf1N1B4_1339 at 1535.536 kb on - strand, within Pf1N1B4_1339 at 1535.961 kb on + strand, within Pf1N1B4_1339 at 1535.961 kb on + strand, within Pf1N1B4_1339 at 1535.961 kb on + strand, within Pf1N1B4_1339 at 1535.962 kb on - strand, within Pf1N1B4_1339 at 1535.962 kb on - strand, within Pf1N1B4_1339 at 1535.962 kb on - strand, within Pf1N1B4_1339 at 1536.037 kb on + strand at 1536.037 kb on + strand at 1536.037 kb on + strand at 1536.037 kb on + strand at 1536.038 kb on - strand at 1536.392 kb on + strand at 1536.393 kb on - strand, within Pf1N1B4_1340 at 1536.393 kb on - strand, within Pf1N1B4_1340 at 1536.515 kb on + strand, within Pf1N1B4_1340 at 1536.516 kb on - strand, within Pf1N1B4_1340 at 1536.578 kb on + strand, within Pf1N1B4_1340 at 1536.578 kb on + strand, within Pf1N1B4_1340 at 1536.579 kb on - strand, within Pf1N1B4_1340 at 1536.881 kb on + strand, within Pf1N1B4_1340 at 1536.983 kb on + strand at 1536.983 kb on + strand at 1536.984 kb on - strand at 1536.984 kb on - strand at 1536.984 kb on - strand at 1536.984 kb on - strand at 1537.069 kb on + strand at 1537.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,534,263 + Pf1N1B4_1338 0.75 -1.3 1,534,264 - Pf1N1B4_1338 0.75 +0.0 1,534,264 - Pf1N1B4_1338 0.75 -0.3 1,534,264 - Pf1N1B4_1338 0.75 +0.0 1,534,264 - Pf1N1B4_1338 0.75 +0.0 1,534,572 - +0.0 1,534,572 - +0.0 1,534,645 - +0.0 1,534,826 + +0.0 1,534,921 + +0.0 1,534,921 + -1.4 1,534,922 - +0.0 1,534,922 - -1.5 1,534,922 - -2.5 1,534,961 - +0.0 1,534,961 - +0.0 1,534,961 - -2.1 1,534,984 + +0.0 1,534,985 - +0.0 1,534,985 - +0.0 1,535,025 + +0.0 1,535,026 - +0.0 1,535,032 + +0.0 1,535,032 + -2.0 1,535,032 + +0.0 1,535,032 + +0.0 1,535,130 - +0.0 1,535,139 - +0.0 1,535,139 - -1.8 1,535,139 - -2.0 1,535,536 - Pf1N1B4_1339 0.41 +0.0 1,535,536 - Pf1N1B4_1339 0.41 +0.0 1,535,961 + Pf1N1B4_1339 0.84 +0.0 1,535,961 + Pf1N1B4_1339 0.84 -1.8 1,535,961 + Pf1N1B4_1339 0.84 -5.2 1,535,962 - Pf1N1B4_1339 0.84 +0.0 1,535,962 - Pf1N1B4_1339 0.84 -3.2 1,535,962 - Pf1N1B4_1339 0.84 +0.0 1,536,037 + +0.0 1,536,037 + +0.0 1,536,037 + +0.0 1,536,037 + +0.0 1,536,038 - -1.7 1,536,392 + -4.1 1,536,393 - Pf1N1B4_1340 0.30 -1.0 1,536,393 - Pf1N1B4_1340 0.30 +0.0 1,536,515 + Pf1N1B4_1340 0.43 -2.4 1,536,516 - Pf1N1B4_1340 0.43 +0.0 1,536,578 + Pf1N1B4_1340 0.49 +0.0 1,536,578 + Pf1N1B4_1340 0.49 +0.0 1,536,579 - Pf1N1B4_1340 0.49 -3.5 1,536,881 + Pf1N1B4_1340 0.81 +0.0 1,536,983 + -1.8 1,536,983 + +0.0 1,536,984 - +0.0 1,536,984 - -1.9 1,536,984 - -1.2 1,536,984 - +0.0 1,537,069 + -1.4 1,537,070 - +0.0
Or see this region's nucleotide sequence