Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_12 and Pf1N1B4_13 are separated by 93 nucleotides Pf1N1B4_13 and Pf1N1B4_14 are separated by 152 nucleotides Pf1N1B4_14 and Pf1N1B4_15 are separated by 11 nucleotides
Pf1N1B4_12 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 13,273 to 14,355
_12
Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216
_13
Pf1N1B4_14 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 15,369 to 16,013
_14
Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443
_15
Position (kb) 14
15
16 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.527 kb on - strand, within Pf1N1B4_12 at 13.527 kb on - strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.935 kb on - strand, within Pf1N1B4_12 at 14.150 kb on - strand, within Pf1N1B4_12 at 14.261 kb on + strand at 14.261 kb on + strand at 14.262 kb on - strand at 14.422 kb on + strand at 14.481 kb on + strand at 14.513 kb on + strand at 14.513 kb on + strand at 14.606 kb on + strand, within Pf1N1B4_13 at 14.606 kb on + strand, within Pf1N1B4_13 at 14.691 kb on - strand, within Pf1N1B4_13 at 14.691 kb on - strand, within Pf1N1B4_13 at 14.738 kb on + strand, within Pf1N1B4_13 at 14.739 kb on - strand, within Pf1N1B4_13 at 14.972 kb on - strand, within Pf1N1B4_13 at 14.972 kb on - strand, within Pf1N1B4_13 at 15.152 kb on + strand at 15.152 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.185 kb on - strand at 15.187 kb on + strand at 15.679 kb on + strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 15.680 kb on - strand, within Pf1N1B4_14 at 16.026 kb on + strand at 16.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 13,526 + Pf1N1B4_12 0.23 -0.0 13,526 + -2.6 13,526 + Pf1N1B4_12 0.23 -0.0 13,526 + Pf1N1B4_12 0.23 -1.9 13,526 + Pf1N1B4_12 0.23 -1.3 13,526 + Pf1N1B4_12 0.23 -1.1 13,526 + Pf1N1B4_12 0.23 -2.2 13,526 + -3.1 13,527 - Pf1N1B4_12 0.23 -1.1 13,527 - Pf1N1B4_12 0.23 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -1.9 13,691 + -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.8 13,692 - Pf1N1B4_12 0.39 -0.0 13,692 - Pf1N1B4_12 0.39 -0.0 13,692 - Pf1N1B4_12 0.39 -2.2 13,692 - Pf1N1B4_12 0.39 -0.0 13,692 - Pf1N1B4_12 0.39 -0.0 13,881 - Pf1N1B4_12 0.56 -0.0 13,881 - Pf1N1B4_12 0.56 -1.2 13,881 - Pf1N1B4_12 0.56 -0.0 13,934 + Pf1N1B4_12 0.61 -0.0 13,934 + Pf1N1B4_12 0.61 -0.0 13,934 + Pf1N1B4_12 0.61 -1.4 13,935 - Pf1N1B4_12 0.61 -1.3 14,150 - Pf1N1B4_12 0.81 -0.0 14,261 + -1.4 14,261 + -1.5 14,262 - -0.0 14,422 + -0.7 14,481 + -2.2 14,513 + -0.0 14,513 + -1.4 14,606 + Pf1N1B4_13 0.20 -5.4 14,606 + Pf1N1B4_13 0.20 -3.2 14,691 - Pf1N1B4_13 0.32 -0.0 14,691 - Pf1N1B4_13 0.32 -0.0 14,738 + Pf1N1B4_13 0.38 -0.0 14,739 - Pf1N1B4_13 0.38 -0.0 14,972 - Pf1N1B4_13 0.68 -0.0 14,972 - Pf1N1B4_13 0.68 -0.0 15,152 + -0.0 15,152 + -1.3 15,184 + -1.4 15,184 + -1.8 15,184 + -0.0 15,185 - -1.9 15,187 + -1.6 15,679 + Pf1N1B4_14 0.48 -0.0 15,680 - Pf1N1B4_14 0.48 -0.0 15,680 - Pf1N1B4_14 0.48 -1.5 16,026 + -1.8 16,027 - -2.5
Or see this region's nucleotide sequence