Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1117 and Pf1N1B4_1118 are separated by 288 nucleotides Pf1N1B4_1118 and Pf1N1B4_1119 are separated by 659 nucleotides
Pf1N1B4_1117 - probable periplasmic protein NMA1059, at 1,252,210 to 1,252,929
_1117
Pf1N1B4_1118 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 1,253,218 to 1,253,400
_1118
Pf1N1B4_1119 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 1,254,060 to 1,255,679
_1119
Position (kb) 1253
1254 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1252.248 kb on - strand at 1252.300 kb on + strand, within Pf1N1B4_1117 at 1252.300 kb on + strand, within Pf1N1B4_1117 at 1252.300 kb on + strand, within Pf1N1B4_1117 at 1252.301 kb on - strand, within Pf1N1B4_1117 at 1252.301 kb on - strand, within Pf1N1B4_1117 at 1252.301 kb on - strand, within Pf1N1B4_1117 at 1252.301 kb on - strand, within Pf1N1B4_1117 at 1252.301 kb on - strand, within Pf1N1B4_1117 at 1252.301 kb on - strand, within Pf1N1B4_1117 at 1252.369 kb on + strand, within Pf1N1B4_1117 at 1252.370 kb on - strand, within Pf1N1B4_1117 at 1252.504 kb on + strand, within Pf1N1B4_1117 at 1252.504 kb on + strand, within Pf1N1B4_1117 at 1252.505 kb on - strand, within Pf1N1B4_1117 at 1252.505 kb on - strand, within Pf1N1B4_1117 at 1252.505 kb on - strand at 1252.505 kb on - strand, within Pf1N1B4_1117 at 1252.751 kb on - strand, within Pf1N1B4_1117 at 1252.751 kb on - strand, within Pf1N1B4_1117 at 1252.798 kb on + strand, within Pf1N1B4_1117 at 1252.798 kb on + strand, within Pf1N1B4_1117 at 1252.798 kb on + strand, within Pf1N1B4_1117 at 1252.798 kb on + strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.799 kb on - strand, within Pf1N1B4_1117 at 1252.936 kb on - strand at 1252.963 kb on + strand at 1252.963 kb on + strand at 1252.963 kb on + strand at 1252.964 kb on - strand at 1252.964 kb on - strand at 1252.964 kb on - strand at 1253.169 kb on + strand at 1253.169 kb on + strand at 1253.169 kb on + strand at 1253.219 kb on + strand at 1253.219 kb on + strand at 1253.219 kb on + strand at 1253.219 kb on + strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.220 kb on - strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.230 kb on + strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.231 kb on - strand at 1253.298 kb on + strand, within Pf1N1B4_1118 at 1253.298 kb on + strand, within Pf1N1B4_1118 at 1253.299 kb on - strand, within Pf1N1B4_1118 at 1253.299 kb on - strand, within Pf1N1B4_1118 at 1253.299 kb on - strand, within Pf1N1B4_1118 at 1253.299 kb on - strand, within Pf1N1B4_1118 at 1253.299 kb on - strand, within Pf1N1B4_1118 at 1253.299 kb on - strand, within Pf1N1B4_1118 at 1253.414 kb on + strand at 1253.414 kb on + strand at 1253.415 kb on - strand at 1253.415 kb on - strand at 1253.415 kb on - strand at 1253.415 kb on - strand at 1253.415 kb on - strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.416 kb on + strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.417 kb on - strand at 1253.469 kb on + strand at 1253.469 kb on + strand at 1253.470 kb on - strand at 1253.472 kb on + strand at 1253.489 kb on + strand at 1253.506 kb on + strand at 1253.506 kb on + strand at 1253.506 kb on + strand at 1253.506 kb on + strand at 1253.506 kb on + strand at 1253.506 kb on + strand at 1253.507 kb on - strand at 1253.507 kb on - strand at 1253.507 kb on - strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.510 kb on + strand at 1253.511 kb on - strand at 1253.511 kb on - strand at 1253.511 kb on - strand at 1253.511 kb on - strand at 1253.511 kb on - strand at 1253.572 kb on + strand at 1253.573 kb on - strand at 1253.573 kb on - strand at 1253.577 kb on + strand at 1253.577 kb on + strand at 1253.577 kb on + strand at 1253.577 kb on + strand at 1253.578 kb on - strand at 1253.647 kb on + strand at 1253.647 kb on + strand at 1253.647 kb on + strand at 1253.647 kb on + strand at 1253.648 kb on - strand at 1253.648 kb on - strand at 1253.648 kb on - strand at 1253.648 kb on - strand at 1253.648 kb on - strand at 1253.648 kb on - strand at 1253.648 kb on - strand at 1253.753 kb on + strand at 1253.753 kb on + strand at 1253.753 kb on + strand at 1253.754 kb on - strand at 1253.836 kb on - strand at 1253.836 kb on - strand at 1253.836 kb on - strand at 1253.894 kb on + strand at 1253.894 kb on + strand at 1253.895 kb on - strand at 1253.895 kb on - strand at 1253.895 kb on - strand at 1253.895 kb on - strand at 1253.957 kb on + strand at 1253.967 kb on + strand at 1253.968 kb on - strand at 1253.968 kb on - strand at 1253.968 kb on - strand at 1253.982 kb on + strand at 1253.993 kb on - strand at 1254.108 kb on - strand at 1254.108 kb on - strand at 1254.165 kb on + strand at 1254.166 kb on - strand at 1254.166 kb on - strand at 1254.208 kb on - strand at 1254.208 kb on - strand at 1254.340 kb on - strand, within Pf1N1B4_1119 at 1254.340 kb on - strand, within Pf1N1B4_1119 at 1254.340 kb on - strand, within Pf1N1B4_1119 at 1254.385 kb on - strand, within Pf1N1B4_1119 at 1254.385 kb on - strand, within Pf1N1B4_1119 at 1254.385 kb on - strand, within Pf1N1B4_1119
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,252,248 - -0.0 1,252,300 + Pf1N1B4_1117 0.12 -3.8 1,252,300 + Pf1N1B4_1117 0.12 -0.0 1,252,300 + Pf1N1B4_1117 0.12 -2.3 1,252,301 - Pf1N1B4_1117 0.13 -2.5 1,252,301 - Pf1N1B4_1117 0.13 -0.0 1,252,301 - Pf1N1B4_1117 0.13 +1.0 1,252,301 - Pf1N1B4_1117 0.13 -0.0 1,252,301 - Pf1N1B4_1117 0.13 +0.4 1,252,301 - Pf1N1B4_1117 0.13 -0.0 1,252,369 + Pf1N1B4_1117 0.22 -2.6 1,252,370 - Pf1N1B4_1117 0.22 -1.0 1,252,504 + Pf1N1B4_1117 0.41 -0.0 1,252,504 + Pf1N1B4_1117 0.41 -2.7 1,252,505 - Pf1N1B4_1117 0.41 -1.3 1,252,505 - Pf1N1B4_1117 0.41 -1.4 1,252,505 - -1.5 1,252,505 - Pf1N1B4_1117 0.41 -0.0 1,252,751 - Pf1N1B4_1117 0.75 -0.0 1,252,751 - Pf1N1B4_1117 0.75 -0.0 1,252,798 + Pf1N1B4_1117 0.82 -0.0 1,252,798 + Pf1N1B4_1117 0.82 -0.0 1,252,798 + Pf1N1B4_1117 0.82 -0.0 1,252,798 + Pf1N1B4_1117 0.82 -0.0 1,252,799 - Pf1N1B4_1117 0.82 -1.1 1,252,799 - Pf1N1B4_1117 0.82 -1.8 1,252,799 - Pf1N1B4_1117 0.82 -3.0 1,252,799 - Pf1N1B4_1117 0.82 -0.8 1,252,799 - Pf1N1B4_1117 0.82 -0.0 1,252,799 - Pf1N1B4_1117 0.82 +1.0 1,252,799 - Pf1N1B4_1117 0.82 -0.0 1,252,799 - Pf1N1B4_1117 0.82 -0.0 1,252,799 - Pf1N1B4_1117 0.82 -0.0 1,252,936 - -0.0 1,252,963 + -0.0 1,252,963 + -2.1 1,252,963 + -1.6 1,252,964 - -0.0 1,252,964 - -2.0 1,252,964 - -0.0 1,253,169 + -1.8 1,253,169 + -0.7 1,253,169 + -0.0 1,253,219 + -0.0 1,253,219 + -1.5 1,253,219 + -2.3 1,253,219 + -0.0 1,253,220 - -0.0 1,253,220 - -0.0 1,253,220 - -0.0 1,253,220 - -0.6 1,253,220 - -1.0 1,253,220 - -0.0 1,253,220 - -0.0 1,253,220 - -2.4 1,253,230 + -0.0 1,253,230 + -2.2 1,253,230 + -0.0 1,253,230 + -0.6 1,253,230 + -0.0 1,253,230 + -0.0 1,253,230 + -1.6 1,253,230 + -0.0 1,253,230 + -0.0 1,253,230 + -0.0 1,253,231 - -0.0 1,253,231 - -0.0 1,253,231 - -2.6 1,253,231 - -0.0 1,253,231 - -3.6 1,253,231 - -1.1 1,253,231 - -0.0 1,253,231 - -1.2 1,253,231 - -0.9 1,253,231 - -2.1 1,253,231 - -1.4 1,253,231 - -0.0 1,253,231 - -0.0 1,253,231 - -1.4 1,253,231 - -2.0 1,253,298 + Pf1N1B4_1118 0.44 -0.0 1,253,298 + Pf1N1B4_1118 0.44 -0.0 1,253,299 - Pf1N1B4_1118 0.44 -0.0 1,253,299 - Pf1N1B4_1118 0.44 -2.3 1,253,299 - Pf1N1B4_1118 0.44 -4.3 1,253,299 - Pf1N1B4_1118 0.44 -0.0 1,253,299 - Pf1N1B4_1118 0.44 -0.0 1,253,299 - Pf1N1B4_1118 0.44 -0.0 1,253,414 + -0.0 1,253,414 + -0.0 1,253,415 - -0.0 1,253,415 - -0.0 1,253,415 - -1.0 1,253,415 - -0.0 1,253,415 - -0.0 1,253,416 + -1.2 1,253,416 + -1.0 1,253,416 + -2.8 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -2.4 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -1.1 1,253,416 + -1.2 1,253,416 + -0.0 1,253,416 + -1.9 1,253,416 + -1.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.0 1,253,416 + -0.9 1,253,416 + -0.0 1,253,416 + -0.0 1,253,417 - -1.6 1,253,417 - -0.5 1,253,417 - -1.4 1,253,417 - -0.0 1,253,417 - -1.5 1,253,417 - -1.6 1,253,417 - -1.7 1,253,417 - -2.0 1,253,417 - -0.0 1,253,417 - -3.1 1,253,417 - -0.0 1,253,417 - -0.0 1,253,417 - -0.0 1,253,417 - -0.0 1,253,417 - -0.0 1,253,417 - -0.0 1,253,417 - -0.0 1,253,417 - -1.7 1,253,417 - -0.0 1,253,417 - -0.9 1,253,417 - -0.0 1,253,417 - -0.0 1,253,469 + -0.0 1,253,469 + -1.4 1,253,470 - -0.0 1,253,472 + -2.5 1,253,489 + -0.0 1,253,506 + -2.9 1,253,506 + -1.7 1,253,506 + -1.3 1,253,506 + -0.0 1,253,506 + -0.0 1,253,506 + -0.0 1,253,507 - -0.0 1,253,507 - -1.1 1,253,507 - -0.0 1,253,510 + -0.0 1,253,510 + -0.0 1,253,510 + -1.9 1,253,510 + -0.0 1,253,510 + -0.0 1,253,510 + -1.7 1,253,510 + -0.0 1,253,510 + -1.5 1,253,511 - -2.8 1,253,511 - -0.0 1,253,511 - -1.4 1,253,511 - -0.0 1,253,511 - -0.0 1,253,572 + -2.0 1,253,573 - -1.8 1,253,573 - -0.0 1,253,577 + -0.0 1,253,577 + -0.0 1,253,577 + -0.0 1,253,577 + -0.0 1,253,578 - -0.0 1,253,647 + -0.0 1,253,647 + -0.0 1,253,647 + -3.1 1,253,647 + -1.3 1,253,648 - -0.0 1,253,648 - -0.0 1,253,648 - -0.0 1,253,648 - -0.0 1,253,648 - -0.0 1,253,648 - -0.0 1,253,648 - -0.0 1,253,753 + -1.9 1,253,753 + -0.4 1,253,753 + -0.0 1,253,754 - -0.0 1,253,836 - -0.0 1,253,836 - -0.0 1,253,836 - -0.0 1,253,894 + -2.1 1,253,894 + -0.0 1,253,895 - -0.0 1,253,895 - -0.0 1,253,895 - -0.0 1,253,895 - -1.0 1,253,957 + -1.3 1,253,967 + -0.0 1,253,968 - -0.0 1,253,968 - -0.0 1,253,968 - -2.2 1,253,982 + -0.0 1,253,993 - -0.0 1,254,108 - -2.2 1,254,108 - -1.8 1,254,165 + -0.0 1,254,166 - -0.0 1,254,166 - -0.0 1,254,208 - -1.2 1,254,208 - -0.0 1,254,340 - Pf1N1B4_1119 0.17 -2.2 1,254,340 - Pf1N1B4_1119 0.17 -0.0 1,254,340 - Pf1N1B4_1119 0.17 -0.0 1,254,385 - Pf1N1B4_1119 0.20 -1.0 1,254,385 - Pf1N1B4_1119 0.20 -0.0 1,254,385 - Pf1N1B4_1119 0.20 -0.0
Or see this region's nucleotide sequence