Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_110

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_108 and Pf1N1B4_109 are separated by 82 nucleotidesPf1N1B4_109 and Pf1N1B4_110 are separated by 17 nucleotidesPf1N1B4_110 and Pf1N1B4_111 are separated by 226 nucleotides Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298 _108 Pf1N1B4_109 - hypothetical protein, at 114,381 to 114,536 _109 Pf1N1B4_110 - Queuosine biosynthesis QueD, PTPS-I, at 114,554 to 114,910 _110 Pf1N1B4_111 - Possible oxidoreductase, at 115,137 to 115,961 _111 Position (kb) 114 115Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 113.961 kb on - strand, within Pf1N1B4_108at 113.979 kb on - strand, within Pf1N1B4_108at 114.116 kb on + strand, within Pf1N1B4_108at 114.116 kb on + strand, within Pf1N1B4_108at 114.116 kb on + strand, within Pf1N1B4_108at 114.116 kb on + strand, within Pf1N1B4_108at 114.200 kb on + strandat 114.201 kb on - strandat 114.234 kb on + strandat 114.315 kb on + strandat 114.316 kb on - strandat 114.494 kb on - strand, within Pf1N1B4_109at 114.740 kb on + strand, within Pf1N1B4_110at 114.740 kb on + strand, within Pf1N1B4_110at 114.741 kb on - strand, within Pf1N1B4_110at 114.757 kb on + strand, within Pf1N1B4_110at 114.758 kb on - strandat 114.758 kb on - strand, within Pf1N1B4_110at 114.758 kb on - strand, within Pf1N1B4_110at 114.758 kb on - strand, within Pf1N1B4_110at 114.758 kb on - strand, within Pf1N1B4_110at 114.761 kb on + strand, within Pf1N1B4_110at 114.761 kb on + strand, within Pf1N1B4_110at 114.762 kb on - strand, within Pf1N1B4_110at 114.762 kb on - strand, within Pf1N1B4_110at 114.762 kb on - strandat 114.896 kb on + strandat 115.064 kb on - strandat 115.099 kb on + strandat 115.099 kb on + strandat 115.099 kb on + strandat 115.099 kb on + strandat 115.099 kb on + strandat 115.099 kb on + strandat 115.100 kb on - strandat 115.100 kb on - strandat 115.100 kb on - strandat 115.165 kb on - strandat 115.198 kb on + strandat 115.251 kb on + strand, within Pf1N1B4_111at 115.251 kb on + strand, within Pf1N1B4_111at 115.251 kb on + strand, within Pf1N1B4_111at 115.251 kb on + strand, within Pf1N1B4_111at 115.252 kb on - strand, within Pf1N1B4_111at 115.252 kb on - strand, within Pf1N1B4_111at 115.252 kb on - strand, within Pf1N1B4_111at 115.255 kb on + strand, within Pf1N1B4_111at 115.255 kb on + strandat 115.255 kb on + strand, within Pf1N1B4_111at 115.255 kb on + strand, within Pf1N1B4_111at 115.256 kb on - strand, within Pf1N1B4_111at 115.256 kb on - strand, within Pf1N1B4_111at 115.256 kb on - strand, within Pf1N1B4_111at 115.256 kb on - strand, within Pf1N1B4_111at 115.256 kb on - strand, within Pf1N1B4_111at 115.303 kb on - strand, within Pf1N1B4_111at 115.303 kb on - strand, within Pf1N1B4_111at 115.321 kb on - strand, within Pf1N1B4_111at 115.363 kb on + strand, within Pf1N1B4_111at 115.482 kb on + strand, within Pf1N1B4_111at 115.587 kb on + strand, within Pf1N1B4_111at 115.615 kb on - strand, within Pf1N1B4_111at 115.615 kb on - strand, within Pf1N1B4_111at 115.616 kb on + strandat 115.616 kb on + strand, within Pf1N1B4_111at 115.617 kb on - strand, within Pf1N1B4_111at 115.617 kb on - strand, within Pf1N1B4_111at 115.617 kb on - strand, within Pf1N1B4_111at 115.665 kb on + strand, within Pf1N1B4_111at 115.665 kb on + strand, within Pf1N1B4_111at 115.665 kb on + strand, within Pf1N1B4_111at 115.665 kb on + strand, within Pf1N1B4_111at 115.666 kb on - strandat 115.708 kb on - strand, within Pf1N1B4_111

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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113,961 - Pf1N1B4_108 0.69 -0.0
113,979 - Pf1N1B4_108 0.70 -0.0
114,116 + Pf1N1B4_108 0.83 -1.5
114,116 + Pf1N1B4_108 0.83 -0.0
114,116 + Pf1N1B4_108 0.83 -2.0
114,116 + Pf1N1B4_108 0.83 -0.0
114,200 + -0.0
114,201 - -0.0
114,234 + -0.0
114,315 + -2.2
114,316 - -0.0
114,494 - Pf1N1B4_109 0.72 -1.8
114,740 + Pf1N1B4_110 0.52 -0.0
114,740 + Pf1N1B4_110 0.52 -0.0
114,741 - Pf1N1B4_110 0.52 -0.0
114,757 + Pf1N1B4_110 0.57 -2.4
114,758 - -0.0
114,758 - Pf1N1B4_110 0.57 -0.0
114,758 - Pf1N1B4_110 0.57 -0.0
114,758 - Pf1N1B4_110 0.57 -0.0
114,758 - Pf1N1B4_110 0.57 -0.0
114,761 + Pf1N1B4_110 0.58 -0.0
114,761 + Pf1N1B4_110 0.58 -2.5
114,762 - Pf1N1B4_110 0.58 -0.0
114,762 - Pf1N1B4_110 0.58 -0.0
114,762 - -0.0
114,896 + -0.0
115,064 - -2.5
115,099 + -2.0
115,099 + -0.0
115,099 + -0.0
115,099 + -0.0
115,099 + -0.0
115,099 + -1.3
115,100 - -1.9
115,100 - -0.0
115,100 - -0.0
115,165 - -0.0
115,198 + -1.5
115,251 + Pf1N1B4_111 0.14 -2.5
115,251 + Pf1N1B4_111 0.14 -0.0
115,251 + Pf1N1B4_111 0.14 -0.0
115,251 + Pf1N1B4_111 0.14 -2.1
115,252 - Pf1N1B4_111 0.14 -0.0
115,252 - Pf1N1B4_111 0.14 -0.0
115,252 - Pf1N1B4_111 0.14 -2.6
115,255 + Pf1N1B4_111 0.14 -0.0
115,255 + -0.0
115,255 + Pf1N1B4_111 0.14 -0.6
115,255 + Pf1N1B4_111 0.14 -0.0
115,256 - Pf1N1B4_111 0.14 -1.9
115,256 - Pf1N1B4_111 0.14 -0.0
115,256 - Pf1N1B4_111 0.14 -0.0
115,256 - Pf1N1B4_111 0.14 -3.3
115,256 - Pf1N1B4_111 0.14 -0.0
115,303 - Pf1N1B4_111 0.20 -1.7
115,303 - Pf1N1B4_111 0.20 -0.0
115,321 - Pf1N1B4_111 0.22 -0.0
115,363 + Pf1N1B4_111 0.27 -0.0
115,482 + Pf1N1B4_111 0.42 -4.3
115,587 + Pf1N1B4_111 0.55 -0.0
115,615 - Pf1N1B4_111 0.58 -1.3
115,615 - Pf1N1B4_111 0.58 -1.7
115,616 + -1.1
115,616 + Pf1N1B4_111 0.58 -2.2
115,617 - Pf1N1B4_111 0.58 -0.0
115,617 - Pf1N1B4_111 0.58 -0.0
115,617 - Pf1N1B4_111 0.58 -1.7
115,665 + Pf1N1B4_111 0.64 -0.0
115,665 + Pf1N1B4_111 0.64 -1.3
115,665 + Pf1N1B4_111 0.64 -0.0
115,665 + Pf1N1B4_111 0.64 -0.0
115,666 - -1.0
115,708 - Pf1N1B4_111 0.69 -0.0

Or see this region's nucleotide sequence