Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3989 and Dshi_3990 overlap by 14 nucleotides Dshi_3990 and Dshi_3991 are separated by 561 nucleotides Dshi_3991 and Dshi_3992 are separated by 1 nucleotides
Dshi_3989: Dshi_3989 - Relaxase/mobilization nuclease family protein (RefSeq), at 50,603 to 52,936
_3989
Dshi_3990: Dshi_3990 - hypothetical protein (RefSeq), at 52,923 to 53,516
_3990
Dshi_3991: Dshi_3991 - hypothetical protein (RefSeq), at 54,078 to 54,284
_3991
Dshi_3992: Dshi_3992 - no description, at 54,286 to 54,797
_3992
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-1
0
1 at 51.939 kb on + strand, within Dshi_3989 at 52.155 kb on + strand, within Dshi_3989 at 52.245 kb on + strand, within Dshi_3989 at 52.515 kb on + strand, within Dshi_3989 at 52.569 kb on + strand, within Dshi_3989 at 52.711 kb on + strand at 52.866 kb on + strand at 52.957 kb on + strand at 53.100 kb on + strand, within Dshi_3990 at 53.101 kb on - strand, within Dshi_3990 at 53.117 kb on - strand, within Dshi_3990 at 53.205 kb on - strand, within Dshi_3990 at 53.365 kb on + strand, within Dshi_3990 at 53.454 kb on + strand, within Dshi_3990 at 53.462 kb on - strand at 53.502 kb on - strand at 53.519 kb on - strand at 53.698 kb on + strand at 53.715 kb on + strand at 53.715 kb on + strand at 53.723 kb on - strand at 53.723 kb on - strand at 53.806 kb on + strand at 53.806 kb on + strand at 53.813 kb on - strand at 54.012 kb on - strand at 54.309 kb on + strand at 54.460 kb on + strand, within Dshi_3992 at 54.469 kb on + strand, within Dshi_3992 at 54.474 kb on + strand, within Dshi_3992 at 54.516 kb on - strand, within Dshi_3992
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 51,939 + Dshi_3989 0.57 -0.1 52,155 + Dshi_3989 0.66 -0.2 52,245 + Dshi_3989 0.70 -0.8 52,515 + Dshi_3989 0.82 -0.5 52,569 + Dshi_3989 0.84 -1.0 52,711 + +0.9 52,866 + -0.2 52,957 + +1.3 53,100 + Dshi_3990 0.30 -0.8 53,101 - Dshi_3990 0.30 +0.2 53,117 - Dshi_3990 0.33 -0.6 53,205 - Dshi_3990 0.47 -0.1 53,365 + Dshi_3990 0.74 -0.3 53,454 + Dshi_3990 0.89 -1.3 53,462 - +0.3 53,502 - +0.3 53,519 - +0.9 53,698 + -0.3 53,715 + +0.6 53,715 + +1.6 53,723 - +0.6 53,723 - +1.1 53,806 + -0.1 53,806 + +0.1 53,813 - +0.6 54,012 - +0.1 54,309 + -0.2 54,460 + Dshi_3992 0.34 +0.3 54,469 + Dshi_3992 0.36 +0.0 54,474 + Dshi_3992 0.37 -0.3 54,516 - Dshi_3992 0.45 -1.0
Or see this region's nucleotide sequence