Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3972 and Dshi_3973 are separated by 43 nucleotides Dshi_3973 and Dshi_3974 overlap by 4 nucleotides Dshi_3974 and Dshi_3975 overlap by 8 nucleotides Dshi_3975 and Dshi_3976 overlap by 11 nucleotides
Dshi_3972: Dshi_3972 - hypothetical protein (RefSeq), at 33,138 to 33,491
_3972
Dshi_3973: Dshi_3973 - Lytic transglycosylase catalytic (RefSeq), at 33,535 to 34,149
_3973
Dshi_3974: Dshi_3974 - Conjugal transfer protein TrbC (RefSeq), at 34,146 to 34,439
_3974
Dshi_3975: Dshi_3975 - type IV secretory pathway VirB3 family protein (RefSeq), at 34,432 to 34,710
_3975
Dshi_3976: Dshi_3976 - CagE TrbE VirB component of type IV transporter system (RefSeq), at 34,700 to 37,063
_3976
Position (kb)
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 33.441 kb on - strand, within Dshi_3972 at 33.476 kb on + strand at 33.481 kb on + strand at 33.484 kb on - strand at 33.499 kb on - strand at 33.538 kb on + strand at 33.543 kb on + strand at 33.563 kb on + strand at 33.563 kb on + strand at 33.563 kb on + strand at 33.581 kb on - strand at 33.614 kb on + strand, within Dshi_3973 at 33.614 kb on + strand, within Dshi_3973 at 33.619 kb on + strand, within Dshi_3973 at 33.619 kb on + strand, within Dshi_3973 at 33.627 kb on - strand, within Dshi_3973 at 33.627 kb on - strand, within Dshi_3973 at 33.632 kb on - strand, within Dshi_3973 at 33.662 kb on - strand, within Dshi_3973 at 33.712 kb on + strand, within Dshi_3973 at 33.712 kb on + strand, within Dshi_3973 at 33.720 kb on - strand, within Dshi_3973 at 33.720 kb on - strand, within Dshi_3973 at 33.720 kb on - strand, within Dshi_3973 at 33.766 kb on + strand, within Dshi_3973 at 33.773 kb on + strand, within Dshi_3973 at 33.778 kb on + strand, within Dshi_3973 at 33.778 kb on + strand, within Dshi_3973 at 33.778 kb on + strand, within Dshi_3973 at 33.786 kb on - strand, within Dshi_3973 at 33.786 kb on - strand, within Dshi_3973 at 33.786 kb on - strand, within Dshi_3973 at 33.822 kb on + strand, within Dshi_3973 at 33.870 kb on + strand, within Dshi_3973 at 33.892 kb on - strand, within Dshi_3973 at 33.957 kb on - strand, within Dshi_3973 at 34.067 kb on - strand, within Dshi_3973 at 34.087 kb on + strand, within Dshi_3973 at 34.087 kb on + strand, within Dshi_3973 at 34.087 kb on + strand, within Dshi_3973 at 34.089 kb on + strand at 34.095 kb on - strand at 34.095 kb on - strand at 34.095 kb on - strand at 34.102 kb on + strand at 34.110 kb on - strand at 34.182 kb on + strand, within Dshi_3974 at 34.210 kb on + strand, within Dshi_3974 at 34.236 kb on - strand, within Dshi_3974 at 34.256 kb on + strand, within Dshi_3974 at 34.269 kb on + strand, within Dshi_3974 at 34.273 kb on + strand, within Dshi_3974 at 34.288 kb on + strand, within Dshi_3974 at 34.355 kb on + strand, within Dshi_3974 at 34.373 kb on - strand, within Dshi_3974 at 34.430 kb on - strand at 34.500 kb on - strand, within Dshi_3975 at 34.502 kb on + strand, within Dshi_3975 at 34.589 kb on - strand, within Dshi_3975 at 34.703 kb on - strand at 34.741 kb on + strand at 34.772 kb on - strand at 34.780 kb on + strand at 34.793 kb on - strand at 34.819 kb on - strand at 34.859 kb on + strand at 34.859 kb on + strand at 34.866 kb on - strand at 34.882 kb on + strand at 34.948 kb on - strand, within Dshi_3976 at 35.063 kb on - strand, within Dshi_3976 at 35.126 kb on + strand, within Dshi_3976 at 35.141 kb on - strand, within Dshi_3976 at 35.152 kb on + strand, within Dshi_3976 at 35.160 kb on - strand, within Dshi_3976 at 35.216 kb on + strand, within Dshi_3976 at 35.227 kb on + strand, within Dshi_3976 at 35.272 kb on - strand, within Dshi_3976 at 35.274 kb on + strand, within Dshi_3976 at 35.275 kb on + strand, within Dshi_3976 at 35.284 kb on + strand, within Dshi_3976 at 35.284 kb on + strand, within Dshi_3976 at 35.284 kb on + strand, within Dshi_3976 at 35.284 kb on - strand, within Dshi_3976 at 35.323 kb on + strand, within Dshi_3976 at 35.323 kb on + strand, within Dshi_3976 at 35.348 kb on - strand, within Dshi_3976 at 35.368 kb on + strand, within Dshi_3976 at 35.400 kb on - strand, within Dshi_3976 at 35.425 kb on + strand, within Dshi_3976 at 35.466 kb on - strand, within Dshi_3976 at 35.522 kb on - strand, within Dshi_3976 at 35.544 kb on - strand, within Dshi_3976 at 35.582 kb on + strand, within Dshi_3976 at 35.668 kb on + strand, within Dshi_3976 at 35.668 kb on + strand, within Dshi_3976
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 33,441 - Dshi_3972 0.86 -0.6 33,476 + -0.6 33,481 + -1.7 33,484 - -0.5 33,499 - +0.6 33,538 + -0.1 33,543 + +2.5 33,563 + +0.9 33,563 + +0.1 33,563 + -0.3 33,581 - -0.4 33,614 + Dshi_3973 0.13 +0.5 33,614 + Dshi_3973 0.13 +0.1 33,619 + Dshi_3973 0.14 -0.8 33,619 + Dshi_3973 0.14 +0.1 33,627 - Dshi_3973 0.15 +0.2 33,627 - Dshi_3973 0.15 -0.8 33,632 - Dshi_3973 0.16 -0.4 33,662 - Dshi_3973 0.21 +1.2 33,712 + Dshi_3973 0.29 -1.0 33,712 + Dshi_3973 0.29 +0.4 33,720 - Dshi_3973 0.30 +1.2 33,720 - Dshi_3973 0.30 +0.2 33,720 - Dshi_3973 0.30 +0.2 33,766 + Dshi_3973 0.38 +0.2 33,773 + Dshi_3973 0.39 +0.3 33,778 + Dshi_3973 0.40 -0.0 33,778 + Dshi_3973 0.40 -0.0 33,778 + Dshi_3973 0.40 +0.3 33,786 - Dshi_3973 0.41 +0.0 33,786 - Dshi_3973 0.41 -0.4 33,786 - Dshi_3973 0.41 +1.2 33,822 + Dshi_3973 0.47 -0.1 33,870 + Dshi_3973 0.54 -0.4 33,892 - Dshi_3973 0.58 +0.0 33,957 - Dshi_3973 0.69 +0.6 34,067 - Dshi_3973 0.87 +0.3 34,087 + Dshi_3973 0.90 -0.5 34,087 + Dshi_3973 0.90 +1.6 34,087 + Dshi_3973 0.90 +1.8 34,089 + +0.5 34,095 - +0.1 34,095 - -0.2 34,095 - +0.5 34,102 + -0.1 34,110 - +2.3 34,182 + Dshi_3974 0.12 -0.3 34,210 + Dshi_3974 0.22 +1.6 34,236 - Dshi_3974 0.31 -0.1 34,256 + Dshi_3974 0.37 +0.1 34,269 + Dshi_3974 0.42 +0.9 34,273 + Dshi_3974 0.43 -0.2 34,288 + Dshi_3974 0.48 +0.7 34,355 + Dshi_3974 0.71 -0.3 34,373 - Dshi_3974 0.77 -0.4 34,430 - -1.8 34,500 - Dshi_3975 0.24 -0.4 34,502 + Dshi_3975 0.25 -1.7 34,589 - Dshi_3975 0.56 +0.5 34,703 - +0.6 34,741 + -0.2 34,772 - -0.8 34,780 + -0.1 34,793 - -0.3 34,819 - -0.4 34,859 + -0.0 34,859 + +0.1 34,866 - -0.5 34,882 + +0.1 34,948 - Dshi_3976 0.10 +0.0 35,063 - Dshi_3976 0.15 -0.0 35,126 + Dshi_3976 0.18 -0.1 35,141 - Dshi_3976 0.19 +1.0 35,152 + Dshi_3976 0.19 -0.9 35,160 - Dshi_3976 0.19 +0.5 35,216 + Dshi_3976 0.22 -0.1 35,227 + Dshi_3976 0.22 -0.7 35,272 - Dshi_3976 0.24 +0.2 35,274 + Dshi_3976 0.24 +0.9 35,275 + Dshi_3976 0.24 +0.6 35,284 + Dshi_3976 0.25 +0.1 35,284 + Dshi_3976 0.25 -0.4 35,284 + Dshi_3976 0.25 +0.5 35,284 - Dshi_3976 0.25 -0.4 35,323 + Dshi_3976 0.26 +0.4 35,323 + Dshi_3976 0.26 +1.3 35,348 - Dshi_3976 0.27 -0.6 35,368 + Dshi_3976 0.28 +0.0 35,400 - Dshi_3976 0.30 +1.5 35,425 + Dshi_3976 0.31 +1.1 35,466 - Dshi_3976 0.32 -0.1 35,522 - Dshi_3976 0.35 +1.3 35,544 - Dshi_3976 0.36 +1.1 35,582 + Dshi_3976 0.37 -0.1 35,668 + Dshi_3976 0.41 -0.7 35,668 + Dshi_3976 0.41 +0.6
Or see this region's nucleotide sequence