Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3202 and Dshi_3203 are separated by 50 nucleotides Dshi_3203 and Dshi_3204 overlap by 4 nucleotides
Dshi_3202: Dshi_3202 - uracil-xanthine permease (RefSeq), at 3,366,483 to 3,367,907
_3202
Dshi_3203: Dshi_3203 - Ureidoglycolate hydrolase (RefSeq), at 3,367,958 to 3,368,452
_3203
Dshi_3204: Dshi_3204 - allantoinase (EC 3.5.2.5); 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97) (from data) , at 3,368,449 to 3,369,861
_3204
Position (kb)
3367
3368
3369 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3366.965 kb on - strand, within Dshi_3202 at 3367.104 kb on - strand, within Dshi_3202 at 3367.173 kb on - strand, within Dshi_3202 at 3367.176 kb on + strand, within Dshi_3202 at 3367.203 kb on + strand, within Dshi_3202 at 3367.210 kb on - strand, within Dshi_3202 at 3367.212 kb on + strand, within Dshi_3202 at 3367.221 kb on - strand, within Dshi_3202 at 3367.238 kb on - strand, within Dshi_3202 at 3367.270 kb on - strand, within Dshi_3202 at 3367.306 kb on - strand, within Dshi_3202 at 3367.357 kb on + strand, within Dshi_3202 at 3367.359 kb on + strand, within Dshi_3202 at 3367.401 kb on + strand, within Dshi_3202 at 3367.419 kb on - strand, within Dshi_3202 at 3367.419 kb on - strand, within Dshi_3202 at 3367.421 kb on + strand, within Dshi_3202 at 3367.421 kb on + strand, within Dshi_3202 at 3367.515 kb on - strand, within Dshi_3202 at 3367.534 kb on - strand, within Dshi_3202 at 3367.534 kb on - strand, within Dshi_3202 at 3367.539 kb on + strand, within Dshi_3202 at 3367.557 kb on + strand, within Dshi_3202 at 3367.706 kb on - strand, within Dshi_3202 at 3367.830 kb on - strand at 3367.898 kb on + strand at 3367.898 kb on + strand at 3367.903 kb on + strand at 3367.939 kb on + strand at 3367.979 kb on - strand at 3367.994 kb on - strand at 3368.024 kb on + strand, within Dshi_3203 at 3368.024 kb on + strand, within Dshi_3203 at 3368.072 kb on + strand, within Dshi_3203 at 3368.124 kb on - strand, within Dshi_3203 at 3368.125 kb on - strand, within Dshi_3203 at 3368.189 kb on + strand, within Dshi_3203 at 3368.215 kb on + strand, within Dshi_3203 at 3368.318 kb on - strand, within Dshi_3203 at 3368.382 kb on + strand, within Dshi_3203 at 3368.453 kb on + strand at 3368.478 kb on + strand at 3368.486 kb on - strand at 3368.545 kb on + strand at 3368.564 kb on + strand at 3368.578 kb on - strand at 3368.609 kb on - strand, within Dshi_3204 at 3368.674 kb on + strand, within Dshi_3204 at 3368.674 kb on + strand, within Dshi_3204 at 3368.681 kb on - strand, within Dshi_3204 at 3368.857 kb on - strand, within Dshi_3204 at 3368.882 kb on + strand, within Dshi_3204 at 3368.882 kb on + strand, within Dshi_3204 at 3368.883 kb on + strand, within Dshi_3204 at 3369.014 kb on + strand, within Dshi_3204 at 3369.121 kb on + strand, within Dshi_3204 at 3369.153 kb on + strand, within Dshi_3204 at 3369.161 kb on - strand, within Dshi_3204 at 3369.161 kb on - strand, within Dshi_3204 at 3369.173 kb on - strand, within Dshi_3204 at 3369.176 kb on + strand, within Dshi_3204 at 3369.196 kb on + strand, within Dshi_3204 at 3369.204 kb on - strand, within Dshi_3204 at 3369.204 kb on - strand, within Dshi_3204 at 3369.218 kb on + strand, within Dshi_3204 at 3369.264 kb on - strand, within Dshi_3204 at 3369.284 kb on + strand, within Dshi_3204 at 3369.289 kb on + strand, within Dshi_3204 at 3369.294 kb on - strand, within Dshi_3204
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 3,366,965 - Dshi_3202 0.34 +0.3 3,367,104 - Dshi_3202 0.44 +0.7 3,367,173 - Dshi_3202 0.48 -0.2 3,367,176 + Dshi_3202 0.49 +0.3 3,367,203 + Dshi_3202 0.51 +0.4 3,367,210 - Dshi_3202 0.51 +0.4 3,367,212 + Dshi_3202 0.51 -0.3 3,367,221 - Dshi_3202 0.52 +0.6 3,367,238 - Dshi_3202 0.53 -0.3 3,367,270 - Dshi_3202 0.55 +0.6 3,367,306 - Dshi_3202 0.58 +0.0 3,367,357 + Dshi_3202 0.61 +0.8 3,367,359 + Dshi_3202 0.61 +0.0 3,367,401 + Dshi_3202 0.64 -0.5 3,367,419 - Dshi_3202 0.66 +0.2 3,367,419 - Dshi_3202 0.66 +2.2 3,367,421 + Dshi_3202 0.66 +0.2 3,367,421 + Dshi_3202 0.66 +0.2 3,367,515 - Dshi_3202 0.72 +1.6 3,367,534 - Dshi_3202 0.74 +0.8 3,367,534 - Dshi_3202 0.74 +0.1 3,367,539 + Dshi_3202 0.74 +0.6 3,367,557 + Dshi_3202 0.75 -0.1 3,367,706 - Dshi_3202 0.86 +0.9 3,367,830 - -0.0 3,367,898 + -0.8 3,367,898 + -1.4 3,367,903 + +0.2 3,367,939 + -0.3 3,367,979 - +1.0 3,367,994 - +0.0 3,368,024 + Dshi_3203 0.13 +0.1 3,368,024 + Dshi_3203 0.13 -0.7 3,368,072 + Dshi_3203 0.23 -0.8 3,368,124 - Dshi_3203 0.34 -0.6 3,368,125 - Dshi_3203 0.34 +0.5 3,368,189 + Dshi_3203 0.47 -0.4 3,368,215 + Dshi_3203 0.52 -0.4 3,368,318 - Dshi_3203 0.73 -2.5 3,368,382 + Dshi_3203 0.86 -0.1 3,368,453 + -0.3 3,368,478 + +0.7 3,368,486 - +0.1 3,368,545 + -0.3 3,368,564 + -0.4 3,368,578 - +0.3 3,368,609 - Dshi_3204 0.11 +0.2 3,368,674 + Dshi_3204 0.16 +0.2 3,368,674 + Dshi_3204 0.16 +0.4 3,368,681 - Dshi_3204 0.16 -0.7 3,368,857 - Dshi_3204 0.29 +0.3 3,368,882 + Dshi_3204 0.31 -0.5 3,368,882 + Dshi_3204 0.31 +0.3 3,368,883 + Dshi_3204 0.31 -0.5 3,369,014 + Dshi_3204 0.40 +0.2 3,369,121 + Dshi_3204 0.48 +0.7 3,369,153 + Dshi_3204 0.50 -0.3 3,369,161 - Dshi_3204 0.50 +0.2 3,369,161 - Dshi_3204 0.50 -0.8 3,369,173 - Dshi_3204 0.51 +1.0 3,369,176 + Dshi_3204 0.51 +0.9 3,369,196 + Dshi_3204 0.53 +0.7 3,369,204 - Dshi_3204 0.53 +0.4 3,369,204 - Dshi_3204 0.53 -0.5 3,369,218 + Dshi_3204 0.54 +2.0 3,369,264 - Dshi_3204 0.58 +0.1 3,369,284 + Dshi_3204 0.59 +0.1 3,369,289 + Dshi_3204 0.59 +0.2 3,369,294 - Dshi_3204 0.60 -0.8
Or see this region's nucleotide sequence