Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2807 and Dshi_2808 are separated by 116 nucleotides Dshi_2808 and Dshi_2809 are separated by 27 nucleotides Dshi_2809 and Dshi_2810 are separated by 81 nucleotides Dshi_2810 and Dshi_2811 overlap by 4 nucleotides
Dshi_2807: Dshi_2807 - monoheme cytochrome c SoxX (RefSeq), at 2,965,359 to 2,965,841
_2807
Dshi_2808: Dshi_2808 - thioredoxin SoxW (RefSeq), at 2,965,958 to 2,966,524
_2808
Dshi_2809: Dshi_2809 - cytochrome c biogenesis protein transmembrane region (RefSeq), at 2,966,552 to 2,967,286
_2809
Dshi_2810: Dshi_2810 - regulatory protein SoxS (RefSeq), at 2,967,368 to 2,967,775
_2810
Dshi_2811: Dshi_2811 - protein of unknown function DUF336 (RefSeq), at 2,967,772 to 2,968,284
_2811
Position (kb)
2966
2967
2968 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2965.564 kb on + strand, within Dshi_2807 at 2965.572 kb on - strand, within Dshi_2807 at 2965.619 kb on - strand, within Dshi_2807 at 2965.664 kb on + strand, within Dshi_2807 at 2965.674 kb on + strand, within Dshi_2807 at 2965.754 kb on - strand, within Dshi_2807 at 2965.766 kb on - strand, within Dshi_2807 at 2965.845 kb on - strand at 2965.895 kb on - strand at 2966.008 kb on + strand at 2966.019 kb on + strand, within Dshi_2808 at 2966.027 kb on - strand, within Dshi_2808 at 2966.118 kb on + strand, within Dshi_2808 at 2966.143 kb on + strand, within Dshi_2808 at 2966.144 kb on + strand, within Dshi_2808 at 2966.144 kb on + strand, within Dshi_2808 at 2966.154 kb on + strand, within Dshi_2808 at 2966.200 kb on + strand, within Dshi_2808 at 2966.208 kb on - strand, within Dshi_2808 at 2966.433 kb on + strand, within Dshi_2808 at 2966.441 kb on - strand, within Dshi_2808 at 2966.441 kb on - strand, within Dshi_2808 at 2966.550 kb on + strand at 2966.798 kb on - strand, within Dshi_2809 at 2966.850 kb on + strand, within Dshi_2809 at 2966.998 kb on - strand, within Dshi_2809 at 2967.027 kb on - strand, within Dshi_2809 at 2967.041 kb on - strand, within Dshi_2809 at 2967.107 kb on - strand, within Dshi_2809 at 2967.119 kb on - strand, within Dshi_2809 at 2967.129 kb on - strand, within Dshi_2809 at 2967.170 kb on + strand, within Dshi_2809 at 2967.197 kb on - strand, within Dshi_2809 at 2967.211 kb on - strand, within Dshi_2809 at 2967.277 kb on + strand at 2967.399 kb on - strand at 2967.410 kb on - strand, within Dshi_2810 at 2967.415 kb on - strand, within Dshi_2810 at 2967.444 kb on + strand, within Dshi_2810 at 2967.489 kb on - strand, within Dshi_2810 at 2967.491 kb on + strand, within Dshi_2810 at 2967.523 kb on - strand, within Dshi_2810 at 2967.528 kb on - strand, within Dshi_2810 at 2967.585 kb on + strand, within Dshi_2810 at 2967.666 kb on - strand, within Dshi_2810 at 2967.666 kb on - strand, within Dshi_2810 at 2967.750 kb on + strand at 2967.750 kb on + strand at 2967.881 kb on - strand, within Dshi_2811 at 2967.931 kb on + strand, within Dshi_2811 at 2968.007 kb on - strand, within Dshi_2811 at 2968.015 kb on + strand, within Dshi_2811 at 2968.062 kb on - strand, within Dshi_2811 at 2968.224 kb on - strand, within Dshi_2811 at 2968.253 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 2,965,564 + Dshi_2807 0.42 -0.2 2,965,572 - Dshi_2807 0.44 +0.1 2,965,619 - Dshi_2807 0.54 -0.3 2,965,664 + Dshi_2807 0.63 -0.4 2,965,674 + Dshi_2807 0.65 -0.5 2,965,754 - Dshi_2807 0.82 -1.5 2,965,766 - Dshi_2807 0.84 +1.5 2,965,845 - -0.2 2,965,895 - +0.3 2,966,008 + -0.3 2,966,019 + Dshi_2808 0.11 +3.5 2,966,027 - Dshi_2808 0.12 -0.6 2,966,118 + Dshi_2808 0.28 -1.0 2,966,143 + Dshi_2808 0.33 -0.5 2,966,144 + Dshi_2808 0.33 -0.2 2,966,144 + Dshi_2808 0.33 -2.2 2,966,154 + Dshi_2808 0.35 +0.0 2,966,200 + Dshi_2808 0.43 +2.1 2,966,208 - Dshi_2808 0.44 +0.9 2,966,433 + Dshi_2808 0.84 +0.4 2,966,441 - Dshi_2808 0.85 +0.1 2,966,441 - Dshi_2808 0.85 +0.5 2,966,550 + +0.3 2,966,798 - Dshi_2809 0.33 -0.1 2,966,850 + Dshi_2809 0.41 -0.9 2,966,998 - Dshi_2809 0.61 -0.4 2,967,027 - Dshi_2809 0.65 -0.1 2,967,041 - Dshi_2809 0.67 -0.7 2,967,107 - Dshi_2809 0.76 -0.2 2,967,119 - Dshi_2809 0.77 +0.8 2,967,129 - Dshi_2809 0.79 -0.2 2,967,170 + Dshi_2809 0.84 -1.4 2,967,197 - Dshi_2809 0.88 -0.8 2,967,211 - Dshi_2809 0.90 +0.1 2,967,277 + +0.1 2,967,399 - -0.1 2,967,410 - Dshi_2810 0.10 +0.0 2,967,415 - Dshi_2810 0.12 -0.1 2,967,444 + Dshi_2810 0.19 +1.0 2,967,489 - Dshi_2810 0.30 +0.0 2,967,491 + Dshi_2810 0.30 +0.1 2,967,523 - Dshi_2810 0.38 +1.3 2,967,528 - Dshi_2810 0.39 +0.9 2,967,585 + Dshi_2810 0.53 -0.1 2,967,666 - Dshi_2810 0.73 +0.4 2,967,666 - Dshi_2810 0.73 -0.2 2,967,750 + -0.3 2,967,750 + +0.3 2,967,881 - Dshi_2811 0.21 -1.7 2,967,931 + Dshi_2811 0.31 -0.1 2,968,007 - Dshi_2811 0.46 -0.1 2,968,015 + Dshi_2811 0.47 -0.6 2,968,062 - Dshi_2811 0.57 +0.6 2,968,224 - Dshi_2811 0.88 -0.5 2,968,253 - +0.1
Or see this region's nucleotide sequence