Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2045 and Dshi_5006 are separated by 3 nucleotides Dshi_5006 and Dshi_2047 are separated by 0 nucleotides Dshi_2047 and Dshi_2048 are separated by 6 nucleotides
Dshi_2045: Dshi_2045 - thiamine pyrophosphate protein central region (RefSeq), at 2,164,196 to 2,165,974
_2045
Dshi_5006: Dshi_5006 - hypothetical protein (NCBI), at 2,165,978 to 2,167,135
_5006
Dshi_2047: Dshi_2047 - hypothetical protein (RefSeq), at 2,167,136 to 2,167,762
_2047
Dshi_2048: Dshi_2048 - Mammalian cell entry related domain protein (RefSeq), at 2,167,769 to 2,169,508
_2048
Position (kb)
2165
2166
2167
2168 Strain fitness (log2 ratio)
-1
0
1 at 2166.217 kb on + strand, within Dshi_5006 at 2166.217 kb on + strand, within Dshi_5006 at 2166.339 kb on - strand, within Dshi_5006 at 2166.794 kb on - strand, within Dshi_5006 at 2167.036 kb on - strand at 2167.037 kb on + strand at 2167.181 kb on - strand at 2167.355 kb on - strand, within Dshi_2047 at 2167.472 kb on + strand, within Dshi_2047 at 2167.552 kb on - strand, within Dshi_2047 at 2167.975 kb on - strand, within Dshi_2048 at 2168.095 kb on + strand, within Dshi_2048
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 2,166,217 + Dshi_5006 0.21 -1.4 2,166,217 + Dshi_5006 0.21 -0.1 2,166,339 - Dshi_5006 0.31 -1.6 2,166,794 - Dshi_5006 0.70 -0.2 2,167,036 - -0.1 2,167,037 + -0.2 2,167,181 - -0.1 2,167,355 - Dshi_2047 0.35 -1.3 2,167,472 + Dshi_2047 0.54 +0.4 2,167,552 - Dshi_2047 0.66 -0.5 2,167,975 - Dshi_2048 0.12 -0.3 2,168,095 + Dshi_2048 0.19 +0.1
Or see this region's nucleotide sequence