Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1842 and Dshi_1843 are separated by 99 nucleotides Dshi_1843 and Dshi_1844 are separated by 104 nucleotides Dshi_1844 and Dshi_1845 are separated by 56 nucleotides
Dshi_1842: Dshi_1842 - adenylosuccinate lyase (RefSeq), at 1,923,555 to 1,924,907
_1842
Dshi_1843: Dshi_1843 - hypothetical protein (RefSeq), at 1,925,007 to 1,925,174
_1843
Dshi_1844: Dshi_1844 - Tetratricopeptide TPR_2 repeat protein (RefSeq), at 1,925,279 to 1,925,812
_1844
Dshi_1845: Dshi_1845 - flagellar motor switch protein FliG (RefSeq), at 1,925,869 to 1,926,918
_1845
Position (kb)
1925
1926 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1924.974 kb on + strand at 1925.056 kb on + strand, within Dshi_1843 at 1925.064 kb on - strand, within Dshi_1843 at 1925.111 kb on - strand, within Dshi_1843 at 1925.257 kb on + strand at 1925.265 kb on - strand at 1925.318 kb on + strand at 1925.398 kb on - strand, within Dshi_1844 at 1925.443 kb on - strand, within Dshi_1844 at 1925.614 kb on + strand, within Dshi_1844 at 1925.662 kb on + strand, within Dshi_1844 at 1925.670 kb on - strand, within Dshi_1844 at 1925.762 kb on + strand at 1925.967 kb on - strand at 1926.051 kb on + strand, within Dshi_1845 at 1926.083 kb on - strand, within Dshi_1845 at 1926.116 kb on + strand, within Dshi_1845 at 1926.120 kb on + strand, within Dshi_1845 at 1926.124 kb on - strand, within Dshi_1845 at 1926.298 kb on + strand, within Dshi_1845 at 1926.352 kb on - strand, within Dshi_1845 at 1926.604 kb on - strand, within Dshi_1845 at 1926.674 kb on + strand, within Dshi_1845 at 1926.682 kb on - strand, within Dshi_1845 at 1926.702 kb on - strand, within Dshi_1845
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,924,974 + +0.2 1,925,056 + Dshi_1843 0.29 +0.2 1,925,064 - Dshi_1843 0.34 -0.2 1,925,111 - Dshi_1843 0.62 -0.4 1,925,257 + +0.3 1,925,265 - +0.2 1,925,318 + +1.3 1,925,398 - Dshi_1844 0.22 -1.8 1,925,443 - Dshi_1844 0.31 -0.6 1,925,614 + Dshi_1844 0.63 +0.0 1,925,662 + Dshi_1844 0.72 -0.1 1,925,670 - Dshi_1844 0.73 +0.2 1,925,762 + +1.0 1,925,967 - +0.8 1,926,051 + Dshi_1845 0.17 +3.4 1,926,083 - Dshi_1845 0.20 -0.3 1,926,116 + Dshi_1845 0.24 +0.0 1,926,120 + Dshi_1845 0.24 +0.4 1,926,124 - Dshi_1845 0.24 +0.5 1,926,298 + Dshi_1845 0.41 +0.2 1,926,352 - Dshi_1845 0.46 -0.2 1,926,604 - Dshi_1845 0.70 -0.1 1,926,674 + Dshi_1845 0.77 -1.2 1,926,682 - Dshi_1845 0.77 -0.2 1,926,702 - Dshi_1845 0.79 +0.1
Or see this region's nucleotide sequence