Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1637 and Dshi_1638 are separated by 26 nucleotides Dshi_1638 and Dshi_1639 are separated by 61 nucleotides Dshi_1639 and Dshi_1640 overlap by 4 nucleotides Dshi_1640 and Dshi_1641 are separated by 12 nucleotides Dshi_1641 and Dshi_1642 are separated by 4 nucleotides
Dshi_1637: Dshi_1637 - protein of unknown function DUF161 (RefSeq), at 1,699,686 to 1,700,312
_1637
Dshi_1638: Dshi_1638 - small multidrug resistance protein (RefSeq), at 1,700,339 to 1,700,668
_1638
Dshi_1639: Dshi_1639 - hypothetical protein (RefSeq), at 1,700,730 to 1,701,272
_1639
Dshi_1640: Dshi_1640 - hypothetical protein (RefSeq), at 1,701,269 to 1,701,574
_1640
Dshi_1641: Dshi_1641 - protein of unknown function DUF1330 (RefSeq), at 1,701,587 to 1,701,880
_1641
Dshi_1642: Dshi_1642 - alanyl-tRNA synthetase (RefSeq), at 1,701,885 to 1,704,548
_1642
Position (kb)
1701
1702 Strain fitness (log2 ratio)
-1
0
1
2 at 1700.325 kb on + strand at 1700.329 kb on - strand at 1700.329 kb on - strand at 1700.331 kb on + strand at 1700.336 kb on + strand at 1700.364 kb on - strand at 1700.378 kb on - strand, within Dshi_1638 at 1700.435 kb on + strand, within Dshi_1638 at 1700.456 kb on + strand, within Dshi_1638 at 1700.456 kb on + strand, within Dshi_1638 at 1700.459 kb on - strand, within Dshi_1638 at 1700.509 kb on + strand, within Dshi_1638 at 1700.509 kb on + strand, within Dshi_1638 at 1700.517 kb on - strand, within Dshi_1638 at 1700.517 kb on - strand, within Dshi_1638 at 1700.517 kb on - strand, within Dshi_1638 at 1700.528 kb on + strand, within Dshi_1638 at 1700.580 kb on + strand, within Dshi_1638 at 1700.580 kb on + strand, within Dshi_1638 at 1700.607 kb on - strand, within Dshi_1638 at 1700.644 kb on + strand at 1700.655 kb on + strand at 1700.669 kb on - strand at 1700.735 kb on - strand at 1700.742 kb on + strand at 1700.750 kb on - strand at 1700.772 kb on - strand at 1700.776 kb on - strand at 1700.854 kb on - strand, within Dshi_1639 at 1700.856 kb on + strand, within Dshi_1639 at 1700.864 kb on - strand, within Dshi_1639 at 1700.922 kb on - strand, within Dshi_1639 at 1701.132 kb on + strand, within Dshi_1639 at 1701.132 kb on + strand, within Dshi_1639 at 1701.140 kb on - strand, within Dshi_1639 at 1701.140 kb on - strand, within Dshi_1639 at 1701.275 kb on - strand at 1701.515 kb on - strand, within Dshi_1640 at 1701.647 kb on + strand, within Dshi_1641 at 1701.746 kb on - strand, within Dshi_1641
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,700,325 + +0.1 1,700,329 - -0.1 1,700,329 - +0.4 1,700,331 + +0.4 1,700,336 + -0.5 1,700,364 - +1.8 1,700,378 - Dshi_1638 0.12 +0.4 1,700,435 + Dshi_1638 0.29 -0.0 1,700,456 + Dshi_1638 0.35 +0.4 1,700,456 + Dshi_1638 0.35 -0.3 1,700,459 - Dshi_1638 0.36 +0.5 1,700,509 + Dshi_1638 0.52 +0.8 1,700,509 + Dshi_1638 0.52 +1.6 1,700,517 - Dshi_1638 0.54 +1.0 1,700,517 - Dshi_1638 0.54 -1.1 1,700,517 - Dshi_1638 0.54 +0.7 1,700,528 + Dshi_1638 0.57 +0.3 1,700,580 + Dshi_1638 0.73 +0.6 1,700,580 + Dshi_1638 0.73 -1.4 1,700,607 - Dshi_1638 0.81 -0.1 1,700,644 + +0.2 1,700,655 + -1.4 1,700,669 - -0.2 1,700,735 - -0.3 1,700,742 + +0.8 1,700,750 - -0.0 1,700,772 - +0.3 1,700,776 - +0.1 1,700,854 - Dshi_1639 0.23 -0.1 1,700,856 + Dshi_1639 0.23 +0.0 1,700,864 - Dshi_1639 0.25 -0.8 1,700,922 - Dshi_1639 0.35 +0.1 1,701,132 + Dshi_1639 0.74 +0.2 1,701,132 + Dshi_1639 0.74 +0.2 1,701,140 - Dshi_1639 0.76 -0.1 1,701,140 - Dshi_1639 0.76 +1.1 1,701,275 - -0.1 1,701,515 - Dshi_1640 0.80 -0.5 1,701,647 + Dshi_1641 0.20 -0.3 1,701,746 - Dshi_1641 0.54 +0.6
Or see this region's nucleotide sequence