Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1569 and Dshi_1570 are separated by 162 nucleotides Dshi_1570 and Dshi_1571 are separated by 22 nucleotides Dshi_1571 and Dshi_1572 are separated by 161 nucleotides
Dshi_1569: Dshi_1569 - TrkA-N domain protein (RefSeq), at 1,634,542 to 1,635,918
_1569
Dshi_1570: Dshi_1570 - hypothetical protein (RefSeq), at 1,636,081 to 1,636,563
_1570
Dshi_1571: Dshi_1571 - hypothetical protein (RefSeq), at 1,636,586 to 1,637,023
_1571
Dshi_1572: Dshi_1572 - two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq), at 1,637,185 to 1,638,600
_1572
Position (kb)
1636
1637 Strain fitness (log2 ratio)
-1
0
1 at 1636.105 kb on - strand at 1636.170 kb on + strand, within Dshi_1570 at 1636.212 kb on + strand, within Dshi_1570 at 1636.214 kb on + strand, within Dshi_1570 at 1636.314 kb on - strand, within Dshi_1570 at 1636.331 kb on - strand, within Dshi_1570 at 1636.360 kb on + strand, within Dshi_1570 at 1636.389 kb on - strand, within Dshi_1570 at 1636.420 kb on + strand, within Dshi_1570 at 1636.428 kb on - strand, within Dshi_1570 at 1636.445 kb on - strand, within Dshi_1570 at 1636.673 kb on - strand, within Dshi_1571 at 1636.678 kb on - strand, within Dshi_1571 at 1636.824 kb on - strand, within Dshi_1571 at 1636.824 kb on - strand, within Dshi_1571 at 1636.893 kb on + strand, within Dshi_1571 at 1637.121 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,636,105 - -0.1 1,636,170 + Dshi_1570 0.18 +0.6 1,636,212 + Dshi_1570 0.27 -0.1 1,636,214 + Dshi_1570 0.28 -1.0 1,636,314 - Dshi_1570 0.48 -0.4 1,636,331 - Dshi_1570 0.52 +0.4 1,636,360 + Dshi_1570 0.58 -0.4 1,636,389 - Dshi_1570 0.64 -1.3 1,636,420 + Dshi_1570 0.70 -0.6 1,636,428 - Dshi_1570 0.72 -0.8 1,636,445 - Dshi_1570 0.75 +0.6 1,636,673 - Dshi_1571 0.20 +0.0 1,636,678 - Dshi_1571 0.21 -0.2 1,636,824 - Dshi_1571 0.54 +0.6 1,636,824 - Dshi_1571 0.54 -0.6 1,636,893 + Dshi_1571 0.70 +0.0 1,637,121 + +0.9
Or see this region's nucleotide sequence