Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1375 and Dshi_1376 are separated by 56 nucleotides Dshi_1376 and Dshi_1377 overlap by 8 nucleotides Dshi_1377 and Dshi_1378 overlap by 1 nucleotides
Dshi_1375: Dshi_1375 - Ornithine decarboxylase (RefSeq), at 1,428,663 to 1,429,859
_1375
Dshi_1376: Dshi_1376 - Creatininase (RefSeq), at 1,429,916 to 1,430,713
_1376
Dshi_1377: Dshi_1377 - 2OG-Fe(II) oxygenase (RefSeq), at 1,430,706 to 1,431,608
_1377
Dshi_1378: Dshi_1378 - NAD(P)H dehydrogenase (quinone) (RefSeq), at 1,431,608 to 1,432,243
_1378
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-1
0
1 at 1429.153 kb on - strand, within Dshi_1375 at 1429.231 kb on + strand, within Dshi_1375 at 1429.648 kb on + strand, within Dshi_1375 at 1429.954 kb on - strand at 1430.128 kb on + strand, within Dshi_1376 at 1430.169 kb on + strand, within Dshi_1376 at 1430.172 kb on - strand, within Dshi_1376 at 1430.879 kb on + strand, within Dshi_1377 at 1430.886 kb on + strand, within Dshi_1377 at 1430.966 kb on + strand, within Dshi_1377 at 1431.002 kb on - strand, within Dshi_1377 at 1431.157 kb on - strand, within Dshi_1377 at 1431.170 kb on + strand, within Dshi_1377 at 1431.385 kb on + strand, within Dshi_1377 at 1431.385 kb on + strand, within Dshi_1377 at 1431.393 kb on - strand, within Dshi_1377 at 1431.538 kb on + strand at 1431.551 kb on - strand at 1431.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,429,153 - Dshi_1375 0.41 +1.0 1,429,231 + Dshi_1375 0.47 +0.9 1,429,648 + Dshi_1375 0.82 +0.3 1,429,954 - -0.4 1,430,128 + Dshi_1376 0.27 -0.3 1,430,169 + Dshi_1376 0.32 -0.5 1,430,172 - Dshi_1376 0.32 -0.1 1,430,879 + Dshi_1377 0.19 -0.7 1,430,886 + Dshi_1377 0.20 -0.1 1,430,966 + Dshi_1377 0.29 +0.9 1,431,002 - Dshi_1377 0.33 +0.8 1,431,157 - Dshi_1377 0.50 -0.5 1,431,170 + Dshi_1377 0.51 +0.1 1,431,385 + Dshi_1377 0.75 -0.3 1,431,385 + Dshi_1377 0.75 -0.3 1,431,393 - Dshi_1377 0.76 +0.4 1,431,538 + +0.5 1,431,551 - +0.2 1,431,625 + +0.1
Or see this region's nucleotide sequence