Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1173 and Dshi_1174 are separated by 83 nucleotides Dshi_1174 and Dshi_1175 are separated by 84 nucleotides Dshi_1175 and Dshi_1176 overlap by 11 nucleotides
Dshi_1173: Dshi_1173 - NMT1/THI5 like domain protein (RefSeq), at 1,205,414 to 1,206,421
_1173
Dshi_1174: Dshi_1174 - hypothetical protein (RefSeq), at 1,206,505 to 1,207,314
_1174
Dshi_1175: Dshi_1175 - protein of unknown function DUF583 (RefSeq), at 1,207,399 to 1,207,956
_1175
Dshi_1176: Dshi_1176 - peptidase M23B (RefSeq), at 1,207,946 to 1,209,274
_1176
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-2
-1
0
1 at 1205.532 kb on + strand, within Dshi_1173 at 1205.575 kb on + strand, within Dshi_1173 at 1205.825 kb on + strand, within Dshi_1173 at 1205.878 kb on + strand, within Dshi_1173 at 1205.881 kb on - strand, within Dshi_1173 at 1205.886 kb on - strand, within Dshi_1173 at 1205.886 kb on - strand, within Dshi_1173 at 1205.923 kb on - strand, within Dshi_1173 at 1205.923 kb on - strand, within Dshi_1173 at 1206.080 kb on + strand, within Dshi_1173 at 1206.094 kb on - strand, within Dshi_1173 at 1206.146 kb on + strand, within Dshi_1173 at 1206.158 kb on + strand, within Dshi_1173 at 1206.258 kb on + strand, within Dshi_1173 at 1206.266 kb on - strand, within Dshi_1173 at 1206.590 kb on - strand, within Dshi_1174 at 1206.630 kb on + strand, within Dshi_1174 at 1206.726 kb on - strand, within Dshi_1174 at 1207.005 kb on - strand, within Dshi_1174 at 1207.061 kb on + strand, within Dshi_1174 at 1207.527 kb on - strand, within Dshi_1175
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,205,532 + Dshi_1173 0.12 +0.5 1,205,575 + Dshi_1173 0.16 +0.2 1,205,825 + Dshi_1173 0.41 +0.3 1,205,878 + Dshi_1173 0.46 -0.1 1,205,881 - Dshi_1173 0.46 +0.2 1,205,886 - Dshi_1173 0.47 +0.7 1,205,886 - Dshi_1173 0.47 -0.1 1,205,923 - Dshi_1173 0.50 +0.4 1,205,923 - Dshi_1173 0.50 +1.0 1,206,080 + Dshi_1173 0.66 -1.0 1,206,094 - Dshi_1173 0.67 +0.1 1,206,146 + Dshi_1173 0.73 -0.5 1,206,158 + Dshi_1173 0.74 -0.7 1,206,258 + Dshi_1173 0.84 +0.3 1,206,266 - Dshi_1173 0.85 +0.6 1,206,590 - Dshi_1174 0.10 +0.3 1,206,630 + Dshi_1174 0.15 -1.9 1,206,726 - Dshi_1174 0.27 -0.1 1,207,005 - Dshi_1174 0.62 +0.0 1,207,061 + Dshi_1174 0.69 -0.3 1,207,527 - Dshi_1175 0.23 +0.4
Or see this region's nucleotide sequence