Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1115 and Dshi_1116 are separated by 139 nucleotides Dshi_1116 and Dshi_1117 are separated by 81 nucleotides Dshi_1117 and Dshi_1118 overlap by 4 nucleotides Dshi_1118 and Dshi_1119 are separated by 23 nucleotides
Dshi_1115: Dshi_1115 - ATP-dependent metalloprotease FtsH (RefSeq), at 1,139,764 to 1,141,680
_1115
Dshi_1116: Dshi_1116 - hypothetical protein (RefSeq), at 1,141,820 to 1,142,431
_1116
Dshi_1117: Dshi_1117 - thioesterase superfamily protein (RefSeq), at 1,142,513 to 1,142,929
_1117
Dshi_1118: Dshi_1118 - hypothetical protein (RefSeq), at 1,142,926 to 1,143,639
_1118
Dshi_1119: Dshi_1119 - DNA damage response helicase, ligase-associated <small>(from data)</small>, at 1,143,663 to 1,146,086
_1119
Position (kb) 1142
1143 Strain fitness (log2 ratio) -1
0
1 at 1141.632 kb on - strand at 1141.803 kb on + strand at 1142.086 kb on - strand, within Dshi_1116 at 1142.239 kb on + strand, within Dshi_1116 at 1142.295 kb on - strand, within Dshi_1116 at 1142.511 kb on + strand at 1142.770 kb on - strand, within Dshi_1117 at 1142.770 kb on - strand, within Dshi_1117
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,141,632 - -0.7 1,141,803 + -0.1 1,142,086 - Dshi_1116 0.43 +0.4 1,142,239 + Dshi_1116 0.68 +0.2 1,142,295 - Dshi_1116 0.78 +0.2 1,142,511 + +0.8 1,142,770 - Dshi_1117 0.62 -0.2 1,142,770 - Dshi_1117 0.62 -0.6
Or see this region's nucleotide sequence