Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0903 and Dshi_0904 are separated by 6 nucleotides Dshi_0904 and Dshi_5007 are separated by 192 nucleotides Dshi_5007 and Dshi_0905 are separated by 13 nucleotides
Dshi_0903: Dshi_0903 - hypothetical protein (RefSeq), at 926,215 to 927,828
_0903
Dshi_0904: Dshi_0904 - hypothetical protein (RefSeq), at 927,835 to 928,704
_0904
Dshi_5007: Dshi_5007 - hypothetical protein (RefSeq), at 928,897 to 929,556
_5007
Dshi_0905: Dshi_0905 - protein of unknown function UPF0126 (RefSeq), at 929,570 to 930,199
_0905
Position (kb) 927
928
929 Strain fitness (log2 ratio) -1
0
1 at 926.928 kb on + strand, within Dshi_0903 at 927.107 kb on - strand, within Dshi_0903 at 927.109 kb on + strand, within Dshi_0903 at 927.117 kb on - strand, within Dshi_0903 at 927.189 kb on - strand, within Dshi_0903 at 927.425 kb on - strand, within Dshi_0903 at 927.432 kb on - strand, within Dshi_0903 at 927.432 kb on - strand, within Dshi_0903 at 927.432 kb on - strand, within Dshi_0903 at 927.634 kb on + strand, within Dshi_0903 at 927.787 kb on + strand at 927.935 kb on - strand, within Dshi_0904 at 927.991 kb on - strand, within Dshi_0904 at 928.025 kb on - strand, within Dshi_0904 at 928.073 kb on - strand, within Dshi_0904 at 928.094 kb on + strand, within Dshi_0904 at 928.102 kb on - strand, within Dshi_0904 at 928.130 kb on + strand, within Dshi_0904 at 928.134 kb on + strand, within Dshi_0904 at 928.173 kb on + strand, within Dshi_0904 at 928.236 kb on - strand, within Dshi_0904 at 928.332 kb on + strand, within Dshi_0904 at 928.487 kb on + strand, within Dshi_0904 at 928.533 kb on - strand, within Dshi_0904 at 928.559 kb on + strand, within Dshi_0904 at 928.629 kb on + strand at 928.679 kb on + strand at 928.686 kb on - strand at 928.686 kb on - strand at 928.711 kb on + strand at 928.757 kb on + strand at 928.793 kb on - strand at 928.818 kb on + strand at 929.245 kb on - strand, within Dshi_5007 at 929.383 kb on - strand, within Dshi_5007 at 929.550 kb on + strand at 929.580 kb on + strand at 929.679 kb on + strand, within Dshi_0905 at 929.684 kb on + strand, within Dshi_0905
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 926,928 + Dshi_0903 0.44 -0.1 927,107 - Dshi_0903 0.55 +0.2 927,109 + Dshi_0903 0.55 +0.1 927,117 - Dshi_0903 0.56 -0.1 927,189 - Dshi_0903 0.60 +0.5 927,425 - Dshi_0903 0.75 -0.2 927,432 - Dshi_0903 0.75 -0.4 927,432 - Dshi_0903 0.75 -1.5 927,432 - Dshi_0903 0.75 -0.6 927,634 + Dshi_0903 0.88 +0.4 927,787 + +0.1 927,935 - Dshi_0904 0.11 +0.1 927,991 - Dshi_0904 0.18 -0.2 928,025 - Dshi_0904 0.22 +0.0 928,073 - Dshi_0904 0.27 -0.5 928,094 + Dshi_0904 0.30 -0.6 928,102 - Dshi_0904 0.31 +0.9 928,130 + Dshi_0904 0.34 -0.2 928,134 + Dshi_0904 0.34 +0.0 928,173 + Dshi_0904 0.39 -0.6 928,236 - Dshi_0904 0.46 -1.5 928,332 + Dshi_0904 0.57 -0.9 928,487 + Dshi_0904 0.75 +0.1 928,533 - Dshi_0904 0.80 +0.3 928,559 + Dshi_0904 0.83 -0.6 928,629 + +1.0 928,679 + +0.3 928,686 - -0.4 928,686 - +0.1 928,711 + -0.4 928,757 + -0.2 928,793 - -0.6 928,818 + -0.8 929,245 - Dshi_5007 0.53 -0.6 929,383 - Dshi_5007 0.74 +0.2 929,550 + -0.1 929,580 + -0.9 929,679 + Dshi_0905 0.17 +1.2 929,684 + Dshi_0905 0.18 -0.4
Or see this region's nucleotide sequence