Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0857 and Dshi_0858 are separated by 255 nucleotides Dshi_0858 and Dshi_0859 overlap by 4 nucleotides Dshi_0859 and Dshi_0860 overlap by 4 nucleotides
Dshi_0857: Dshi_0857 - transglutaminase domain protein (RefSeq), at 870,602 to 873,940
_0857
Dshi_0858: Dshi_0858 - ABC transporter related (RefSeq), at 874,196 to 874,888
_0858
Dshi_0859: Dshi_0859 - ABC transporter related (RefSeq), at 874,885 to 875,637
_0859
Dshi_0860: Dshi_0860 - inner-membrane translocator (RefSeq), at 875,634 to 876,608
_0860
Position (kb)
874
875 Strain fitness (log2 ratio)
-1
0
1 at 873.283 kb on + strand, within Dshi_0857 at 873.344 kb on - strand, within Dshi_0857 at 873.385 kb on + strand, within Dshi_0857 at 873.420 kb on + strand, within Dshi_0857 at 873.436 kb on - strand, within Dshi_0857 at 873.459 kb on - strand, within Dshi_0857 at 873.596 kb on + strand, within Dshi_0857 at 873.787 kb on + strand at 873.819 kb on + strand at 873.954 kb on - strand at 874.060 kb on - strand at 874.102 kb on + strand at 874.159 kb on + strand at 874.259 kb on - strand at 874.259 kb on - strand at 874.260 kb on + strand at 874.268 kb on - strand, within Dshi_0858 at 874.414 kb on - strand, within Dshi_0858 at 874.965 kb on + strand, within Dshi_0859 at 875.046 kb on - strand, within Dshi_0859 at 875.092 kb on + strand, within Dshi_0859 at 875.114 kb on + strand, within Dshi_0859 at 875.191 kb on - strand, within Dshi_0859 at 875.341 kb on + strand, within Dshi_0859 at 875.349 kb on - strand, within Dshi_0859 at 875.349 kb on - strand, within Dshi_0859 at 875.424 kb on + strand, within Dshi_0859 at 875.442 kb on + strand, within Dshi_0859 at 875.513 kb on + strand, within Dshi_0859 at 875.556 kb on + strand, within Dshi_0859 at 875.684 kb on + strand at 875.705 kb on + strand at 875.871 kb on - strand, within Dshi_0860
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 873,283 + Dshi_0857 0.80 -0.1 873,344 - Dshi_0857 0.82 -1.4 873,385 + Dshi_0857 0.83 +0.4 873,420 + Dshi_0857 0.84 -0.4 873,436 - Dshi_0857 0.85 +0.3 873,459 - Dshi_0857 0.86 -0.0 873,596 + Dshi_0857 0.90 +0.4 873,787 + +0.6 873,819 + +1.1 873,954 - +0.0 874,060 - -0.7 874,102 + +0.5 874,159 + -0.1 874,259 - +0.5 874,259 - -0.0 874,260 + -0.7 874,268 - Dshi_0858 0.10 -0.2 874,414 - Dshi_0858 0.31 -1.3 874,965 + Dshi_0859 0.11 -0.4 875,046 - Dshi_0859 0.21 +0.2 875,092 + Dshi_0859 0.27 +0.7 875,114 + Dshi_0859 0.30 +1.0 875,191 - Dshi_0859 0.41 +0.0 875,341 + Dshi_0859 0.61 +0.2 875,349 - Dshi_0859 0.62 +0.3 875,349 - Dshi_0859 0.62 -0.1 875,424 + Dshi_0859 0.72 -0.1 875,442 + Dshi_0859 0.74 +0.7 875,513 + Dshi_0859 0.83 -0.1 875,556 + Dshi_0859 0.89 +0.3 875,684 + +0.0 875,705 + +0.7 875,871 - Dshi_0860 0.24 +0.5
Or see this region's nucleotide sequence