Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0701 and Dshi_0702 overlap by 4 nucleotides Dshi_0702 and Dshi_0703 overlap by 1 nucleotides Dshi_0703 and Dshi_0704 are separated by 255 nucleotides
Dshi_0701: Dshi_0701 - Methyltransferase type 12 (RefSeq), at 708,169 to 708,822
_0701
Dshi_0702: Dshi_0702 - WbqC-like family protein (RefSeq), at 708,819 to 709,466
_0702
Dshi_0703: Dshi_0703 - hypothetical protein (RefSeq), at 709,466 to 710,386
_0703
Dshi_0704: Dshi_0704 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 710,642 to 711,829
_0704
Position (kb)
709
710
711 Strain fitness (log2 ratio)
-1
0
1 at 708.539 kb on + strand, within Dshi_0701 at 708.582 kb on + strand, within Dshi_0701 at 708.673 kb on + strand, within Dshi_0701 at 708.809 kb on - strand at 708.945 kb on + strand, within Dshi_0702 at 708.945 kb on + strand, within Dshi_0702 at 709.034 kb on - strand, within Dshi_0702 at 709.183 kb on + strand, within Dshi_0702 at 709.319 kb on + strand, within Dshi_0702 at 709.385 kb on + strand, within Dshi_0702 at 709.438 kb on + strand at 709.441 kb on - strand at 709.573 kb on - strand, within Dshi_0703 at 709.685 kb on - strand, within Dshi_0703 at 709.882 kb on + strand, within Dshi_0703 at 709.882 kb on + strand, within Dshi_0703 at 709.890 kb on - strand, within Dshi_0703 at 709.890 kb on - strand, within Dshi_0703 at 710.012 kb on - strand, within Dshi_0703 at 710.019 kb on + strand, within Dshi_0703 at 710.019 kb on + strand, within Dshi_0703 at 710.027 kb on - strand, within Dshi_0703 at 710.027 kb on - strand, within Dshi_0703 at 710.051 kb on + strand, within Dshi_0703 at 710.077 kb on + strand, within Dshi_0703 at 710.078 kb on - strand, within Dshi_0703 at 710.080 kb on - strand, within Dshi_0703 at 710.085 kb on - strand, within Dshi_0703 at 710.170 kb on + strand, within Dshi_0703 at 710.172 kb on + strand, within Dshi_0703 at 710.172 kb on + strand, within Dshi_0703 at 710.196 kb on + strand, within Dshi_0703 at 710.221 kb on - strand, within Dshi_0703 at 710.264 kb on + strand, within Dshi_0703 at 710.268 kb on + strand, within Dshi_0703 at 710.272 kb on - strand, within Dshi_0703 at 710.277 kb on - strand, within Dshi_0703 at 710.293 kb on + strand, within Dshi_0703 at 710.296 kb on - strand at 710.306 kb on - strand at 710.313 kb on - strand at 710.360 kb on + strand at 710.363 kb on + strand at 710.369 kb on - strand at 710.371 kb on - strand at 710.371 kb on - strand at 710.458 kb on - strand at 710.492 kb on - strand at 710.504 kb on - strand at 710.504 kb on - strand at 710.527 kb on - strand at 710.545 kb on - strand at 710.588 kb on - strand at 710.706 kb on + strand at 710.728 kb on - strand at 710.914 kb on + strand, within Dshi_0704 at 710.923 kb on - strand, within Dshi_0704 at 710.923 kb on - strand, within Dshi_0704 at 710.966 kb on - strand, within Dshi_0704 at 711.091 kb on - strand, within Dshi_0704 at 711.107 kb on + strand, within Dshi_0704 at 711.153 kb on + strand, within Dshi_0704 at 711.164 kb on + strand, within Dshi_0704 at 711.169 kb on + strand, within Dshi_0704 at 711.257 kb on + strand, within Dshi_0704 at 711.279 kb on + strand at 711.289 kb on + strand, within Dshi_0704 at 711.294 kb on - strand, within Dshi_0704
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 708,539 + Dshi_0701 0.57 -0.1 708,582 + Dshi_0701 0.63 +0.4 708,673 + Dshi_0701 0.77 -0.7 708,809 - +0.0 708,945 + Dshi_0702 0.19 -0.3 708,945 + Dshi_0702 0.19 -0.7 709,034 - Dshi_0702 0.33 +0.4 709,183 + Dshi_0702 0.56 -0.9 709,319 + Dshi_0702 0.77 -0.9 709,385 + Dshi_0702 0.87 +0.5 709,438 + +0.2 709,441 - +0.2 709,573 - Dshi_0703 0.12 +0.4 709,685 - Dshi_0703 0.24 -0.7 709,882 + Dshi_0703 0.45 -0.3 709,882 + Dshi_0703 0.45 -1.0 709,890 - Dshi_0703 0.46 -0.8 709,890 - Dshi_0703 0.46 +0.1 710,012 - Dshi_0703 0.59 -0.3 710,019 + Dshi_0703 0.60 -0.3 710,019 + Dshi_0703 0.60 -0.0 710,027 - Dshi_0703 0.61 -0.2 710,027 - Dshi_0703 0.61 -0.6 710,051 + Dshi_0703 0.64 -0.2 710,077 + Dshi_0703 0.66 -0.6 710,078 - Dshi_0703 0.66 +0.0 710,080 - Dshi_0703 0.67 +0.3 710,085 - Dshi_0703 0.67 -0.7 710,170 + Dshi_0703 0.76 -1.5 710,172 + Dshi_0703 0.77 +1.6 710,172 + Dshi_0703 0.77 +0.1 710,196 + Dshi_0703 0.79 -0.2 710,221 - Dshi_0703 0.82 -0.4 710,264 + Dshi_0703 0.87 -0.2 710,268 + Dshi_0703 0.87 -0.3 710,272 - Dshi_0703 0.88 +1.0 710,277 - Dshi_0703 0.88 +0.5 710,293 + Dshi_0703 0.90 -0.0 710,296 - +0.1 710,306 - +0.9 710,313 - +0.9 710,360 + -1.6 710,363 + -1.5 710,369 - -0.8 710,371 - -0.1 710,371 - -0.2 710,458 - +0.3 710,492 - -0.1 710,504 - -0.3 710,504 - -0.1 710,527 - -0.2 710,545 - +1.1 710,588 - -0.3 710,706 + -0.2 710,728 - -0.3 710,914 + Dshi_0704 0.23 +0.5 710,923 - Dshi_0704 0.24 -0.1 710,923 - Dshi_0704 0.24 -0.0 710,966 - Dshi_0704 0.27 +0.6 711,091 - Dshi_0704 0.38 +0.5 711,107 + Dshi_0704 0.39 +0.2 711,153 + Dshi_0704 0.43 -0.7 711,164 + Dshi_0704 0.44 +1.3 711,169 + Dshi_0704 0.44 +0.8 711,257 + Dshi_0704 0.52 +0.0 711,279 + +0.3 711,289 + Dshi_0704 0.54 +0.1 711,294 - Dshi_0704 0.55 -0.1
Or see this region's nucleotide sequence