Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0531 and Dshi_0532 are separated by 223 nucleotides Dshi_0532 and Dshi_0533 overlap by 1 nucleotides Dshi_0533 and Dshi_0534 are separated by 22 nucleotides
Dshi_0531: Dshi_0531 - ABC sugar transporter, periplasmic ligand binding protein (RefSeq), at 515,852 to 516,841
_0531
Dshi_0532: Dshi_0532 - transcriptional regulator, DeoR family (RefSeq), at 517,065 to 518,039
_0532
Dshi_0533: Dshi_0533 - Xylose isomerase domain protein TIM barrel (RefSeq), at 518,039 to 518,887
_0533
Dshi_0534: Dshi_0534 - dehydrogenase E1 component (RefSeq), at 518,910 to 519,929
_0534
Position (kb)
517
518
519 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 516.119 kb on + strand, within Dshi_0531 at 516.234 kb on + strand, within Dshi_0531 at 516.242 kb on - strand, within Dshi_0531 at 516.262 kb on - strand, within Dshi_0531 at 516.325 kb on + strand, within Dshi_0531 at 516.345 kb on + strand, within Dshi_0531 at 516.396 kb on + strand, within Dshi_0531 at 516.527 kb on + strand, within Dshi_0531 at 516.535 kb on - strand, within Dshi_0531 at 516.585 kb on - strand, within Dshi_0531 at 516.593 kb on - strand, within Dshi_0531 at 516.649 kb on + strand, within Dshi_0531 at 516.750 kb on + strand at 516.776 kb on - strand at 516.776 kb on - strand at 516.815 kb on + strand at 516.815 kb on + strand at 516.815 kb on + strand at 516.823 kb on - strand at 516.848 kb on - strand at 516.899 kb on - strand at 516.946 kb on - strand at 516.951 kb on + strand at 516.951 kb on - strand at 517.069 kb on - strand at 517.089 kb on + strand at 517.141 kb on + strand at 517.175 kb on + strand, within Dshi_0532 at 517.210 kb on + strand, within Dshi_0532 at 517.218 kb on - strand, within Dshi_0532 at 517.282 kb on - strand, within Dshi_0532 at 517.595 kb on - strand, within Dshi_0532 at 517.650 kb on + strand, within Dshi_0532 at 517.695 kb on + strand, within Dshi_0532 at 517.703 kb on - strand, within Dshi_0532 at 517.713 kb on - strand, within Dshi_0532 at 517.736 kb on + strand, within Dshi_0532 at 517.751 kb on + strand, within Dshi_0532 at 517.812 kb on - strand, within Dshi_0532 at 517.824 kb on + strand, within Dshi_0532 at 517.872 kb on - strand, within Dshi_0532 at 517.930 kb on + strand, within Dshi_0532 at 517.942 kb on + strand at 517.942 kb on + strand at 517.950 kb on + strand at 517.950 kb on - strand at 517.995 kb on - strand at 518.007 kb on - strand at 518.022 kb on - strand at 518.031 kb on + strand at 518.079 kb on + strand at 518.087 kb on - strand at 518.091 kb on - strand at 518.091 kb on - strand at 518.091 kb on - strand at 518.091 kb on - strand at 518.116 kb on + strand at 518.117 kb on + strand at 518.124 kb on - strand, within Dshi_0533 at 518.124 kb on - strand, within Dshi_0533 at 518.161 kb on - strand, within Dshi_0533 at 518.175 kb on - strand, within Dshi_0533 at 518.275 kb on - strand, within Dshi_0533 at 518.292 kb on + strand, within Dshi_0533 at 518.322 kb on + strand, within Dshi_0533 at 518.363 kb on - strand, within Dshi_0533 at 518.414 kb on + strand, within Dshi_0533 at 518.437 kb on + strand, within Dshi_0533 at 518.497 kb on + strand, within Dshi_0533 at 518.525 kb on - strand, within Dshi_0533 at 518.541 kb on + strand, within Dshi_0533 at 518.546 kb on + strand, within Dshi_0533 at 518.571 kb on - strand, within Dshi_0533 at 518.599 kb on - strand, within Dshi_0533 at 518.635 kb on + strand, within Dshi_0533 at 518.665 kb on + strand, within Dshi_0533 at 518.672 kb on + strand, within Dshi_0533 at 518.685 kb on - strand, within Dshi_0533 at 518.755 kb on - strand, within Dshi_0533 at 518.811 kb on + strand at 518.849 kb on + strand at 518.881 kb on + strand at 518.892 kb on + strand at 518.910 kb on - strand at 518.934 kb on - strand at 518.935 kb on - strand at 518.982 kb on + strand at 518.987 kb on + strand at 518.999 kb on - strand at 519.018 kb on - strand, within Dshi_0534 at 519.021 kb on - strand, within Dshi_0534
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 516,119 + Dshi_0531 0.27 +0.1 516,234 + Dshi_0531 0.39 +0.7 516,242 - Dshi_0531 0.39 -0.4 516,262 - Dshi_0531 0.41 -0.2 516,325 + Dshi_0531 0.48 +0.3 516,345 + Dshi_0531 0.50 -0.5 516,396 + Dshi_0531 0.55 -1.4 516,527 + Dshi_0531 0.68 +0.7 516,535 - Dshi_0531 0.69 -0.1 516,585 - Dshi_0531 0.74 -0.6 516,593 - Dshi_0531 0.75 +0.3 516,649 + Dshi_0531 0.81 -1.6 516,750 + +0.5 516,776 - -0.4 516,776 - -0.3 516,815 + +1.0 516,815 + +0.6 516,815 + -0.2 516,823 - -0.5 516,848 - +2.6 516,899 - -0.2 516,946 - +0.9 516,951 + -0.4 516,951 - -0.5 517,069 - +0.1 517,089 + +1.0 517,141 + -0.3 517,175 + Dshi_0532 0.11 +0.3 517,210 + Dshi_0532 0.15 -0.5 517,218 - Dshi_0532 0.16 +0.2 517,282 - Dshi_0532 0.22 -0.7 517,595 - Dshi_0532 0.54 -1.2 517,650 + Dshi_0532 0.60 +0.0 517,695 + Dshi_0532 0.65 +0.1 517,703 - Dshi_0532 0.65 -0.0 517,713 - Dshi_0532 0.66 +0.0 517,736 + Dshi_0532 0.69 +1.6 517,751 + Dshi_0532 0.70 +0.0 517,812 - Dshi_0532 0.77 -1.4 517,824 + Dshi_0532 0.78 -1.0 517,872 - Dshi_0532 0.83 +0.8 517,930 + Dshi_0532 0.89 -0.8 517,942 + -0.4 517,942 + +0.0 517,950 + +1.0 517,950 - +0.3 517,995 - +0.2 518,007 - -0.0 518,022 - -1.4 518,031 + -0.6 518,079 + +0.1 518,087 - -0.6 518,091 - -0.1 518,091 - -0.2 518,091 - +0.3 518,091 - -0.0 518,116 + -0.2 518,117 + +0.6 518,124 - Dshi_0533 0.10 +0.7 518,124 - Dshi_0533 0.10 -0.2 518,161 - Dshi_0533 0.14 -0.2 518,175 - Dshi_0533 0.16 +0.6 518,275 - Dshi_0533 0.28 -0.4 518,292 + Dshi_0533 0.30 -2.0 518,322 + Dshi_0533 0.33 -1.4 518,363 - Dshi_0533 0.38 +0.2 518,414 + Dshi_0533 0.44 -1.2 518,437 + Dshi_0533 0.47 +0.1 518,497 + Dshi_0533 0.54 -0.5 518,525 - Dshi_0533 0.57 +0.4 518,541 + Dshi_0533 0.59 +0.4 518,546 + Dshi_0533 0.60 +0.2 518,571 - Dshi_0533 0.63 +1.6 518,599 - Dshi_0533 0.66 -0.3 518,635 + Dshi_0533 0.70 +0.1 518,665 + Dshi_0533 0.74 +0.9 518,672 + Dshi_0533 0.75 +0.4 518,685 - Dshi_0533 0.76 -1.4 518,755 - Dshi_0533 0.84 -0.5 518,811 + +1.3 518,849 + +0.0 518,881 + +0.9 518,892 + +0.3 518,910 - -1.5 518,934 - -0.6 518,935 - +0.0 518,982 + +0.1 518,987 + +0.3 518,999 - +0.9 519,018 - Dshi_0534 0.11 +0.4 519,021 - Dshi_0534 0.11 +0.3
Or see this region's nucleotide sequence