Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0409 and Dshi_0410 are separated by 333 nucleotides Dshi_0410 and Dshi_0411 overlap by 17 nucleotides
Dshi_0409: Dshi_0409 - hypothetical protein (RefSeq), at 403,268 to 403,912
_0409
Dshi_0410: Dshi_0410 - hypothetical protein (RefSeq), at 404,246 to 405,190
_0410
Dshi_0411: Dshi_0411 - hypothetical protein (RefSeq), at 405,174 to 406,022
_0411
Position (kb) 404
405
406 Strain fitness (log2 ratio) -1
0
1 at 403.267 kb on + strand at 403.376 kb on - strand, within Dshi_0409 at 403.485 kb on + strand, within Dshi_0409 at 403.502 kb on + strand, within Dshi_0409 at 403.521 kb on + strand, within Dshi_0409 at 403.564 kb on + strand, within Dshi_0409 at 403.649 kb on - strand, within Dshi_0409 at 403.653 kb on - strand, within Dshi_0409 at 403.667 kb on - strand, within Dshi_0409 at 403.677 kb on + strand, within Dshi_0409 at 403.709 kb on + strand, within Dshi_0409 at 403.745 kb on + strand, within Dshi_0409 at 403.774 kb on + strand, within Dshi_0409 at 403.774 kb on + strand, within Dshi_0409 at 403.782 kb on - strand, within Dshi_0409 at 403.909 kb on - strand at 403.951 kb on + strand at 403.975 kb on + strand at 404.008 kb on - strand at 404.064 kb on - strand at 404.075 kb on - strand at 404.086 kb on + strand at 404.140 kb on + strand at 404.244 kb on + strand at 404.252 kb on + strand at 404.252 kb on + strand at 404.252 kb on - strand at 404.260 kb on - strand at 404.311 kb on - strand at 404.337 kb on - strand at 404.413 kb on + strand, within Dshi_0410 at 404.525 kb on - strand, within Dshi_0410 at 404.709 kb on + strand, within Dshi_0410 at 404.743 kb on + strand, within Dshi_0410 at 404.789 kb on - strand, within Dshi_0410 at 404.807 kb on + strand, within Dshi_0410 at 404.959 kb on - strand, within Dshi_0410 at 405.043 kb on + strand, within Dshi_0410 at 405.206 kb on + strand at 405.322 kb on + strand, within Dshi_0411 at 405.332 kb on + strand, within Dshi_0411 at 405.335 kb on + strand, within Dshi_0411 at 405.404 kb on - strand, within Dshi_0411 at 405.675 kb on - strand, within Dshi_0411 at 405.675 kb on - strand, within Dshi_0411 at 405.785 kb on - strand, within Dshi_0411 at 405.816 kb on + strand, within Dshi_0411 at 405.823 kb on + strand, within Dshi_0411 at 405.865 kb on + strand, within Dshi_0411 at 405.941 kb on + strand at 405.949 kb on - strand at 405.949 kb on - strand at 406.011 kb on + strand at 406.011 kb on + strand at 406.145 kb on + strand at 406.146 kb on + strand at 406.160 kb on - strand at 406.176 kb on - strand at 406.180 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 403,267 + +1.1 403,376 - Dshi_0409 0.17 +1.0 403,485 + Dshi_0409 0.34 -0.2 403,502 + Dshi_0409 0.36 +0.1 403,521 + Dshi_0409 0.39 +0.6 403,564 + Dshi_0409 0.46 +0.1 403,649 - Dshi_0409 0.59 -0.0 403,653 - Dshi_0409 0.60 +0.5 403,667 - Dshi_0409 0.62 -0.3 403,677 + Dshi_0409 0.63 -0.2 403,709 + Dshi_0409 0.68 +0.8 403,745 + Dshi_0409 0.74 +0.4 403,774 + Dshi_0409 0.78 +0.0 403,774 + Dshi_0409 0.78 -0.1 403,782 - Dshi_0409 0.80 +0.2 403,909 - +0.9 403,951 + +0.6 403,975 + +0.8 404,008 - -0.1 404,064 - +0.5 404,075 - -0.5 404,086 + +0.1 404,140 + +0.5 404,244 + +0.2 404,252 + +0.5 404,252 + -0.2 404,252 - -0.1 404,260 - -1.0 404,311 - +0.3 404,337 - -0.2 404,413 + Dshi_0410 0.18 -0.5 404,525 - Dshi_0410 0.30 -0.6 404,709 + Dshi_0410 0.49 -0.7 404,743 + Dshi_0410 0.53 +0.6 404,789 - Dshi_0410 0.57 -0.1 404,807 + Dshi_0410 0.59 -0.1 404,959 - Dshi_0410 0.75 -0.5 405,043 + Dshi_0410 0.84 +0.4 405,206 + +0.9 405,322 + Dshi_0411 0.17 +0.2 405,332 + Dshi_0411 0.19 +0.5 405,335 + Dshi_0411 0.19 -0.5 405,404 - Dshi_0411 0.27 +1.2 405,675 - Dshi_0411 0.59 -1.1 405,675 - Dshi_0411 0.59 -0.4 405,785 - Dshi_0411 0.72 +0.4 405,816 + Dshi_0411 0.76 +1.4 405,823 + Dshi_0411 0.76 -0.0 405,865 + Dshi_0411 0.81 -0.1 405,941 + -0.1 405,949 - +0.7 405,949 - -0.2 406,011 + -0.7 406,011 + +0.7 406,145 + +0.0 406,146 + -0.3 406,160 - +0.1 406,176 - -0.1 406,180 + +1.1
Or see this region's nucleotide sequence