Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0245 and Dshi_0246 are separated by 91 nucleotides Dshi_0246 and Dshi_0247 are separated by 121 nucleotides Dshi_0247 and Dshi_0248 are separated by 10 nucleotides
Dshi_0245: Dshi_0245 - transcriptional regulator, AsnC family (RefSeq), at 243,155 to 243,664
_0245
Dshi_0246: Dshi_0246 - ribosomal protein S21 (RefSeq), at 243,756 to 243,962
_0246
Dshi_0247: Dshi_0247 - rpsU-divergently transcribed protein (RefSeq), at 244,084 to 244,782
_0247
Dshi_0248: Dshi_0248 - Quinone oxidoreductase putative PIG3 (RefSeq), at 244,793 to 245,779
_0248
Position (kb) 244
245 Strain fitness (log2 ratio) -2
-1
0
1
2 at 243.130 kb on - strand at 243.146 kb on + strand at 243.244 kb on + strand, within Dshi_0245 at 243.265 kb on + strand, within Dshi_0245 at 243.280 kb on + strand, within Dshi_0245 at 243.301 kb on + strand, within Dshi_0245 at 243.310 kb on - strand, within Dshi_0245 at 243.423 kb on + strand, within Dshi_0245 at 243.485 kb on - strand, within Dshi_0245 at 243.610 kb on - strand, within Dshi_0245 at 243.645 kb on - strand at 243.754 kb on + strand at 244.046 kb on - strand at 244.071 kb on + strand at 244.172 kb on - strand, within Dshi_0247 at 244.185 kb on + strand, within Dshi_0247 at 244.218 kb on + strand, within Dshi_0247 at 244.218 kb on + strand, within Dshi_0247 at 244.307 kb on - strand, within Dshi_0247 at 244.322 kb on + strand, within Dshi_0247 at 244.372 kb on - strand, within Dshi_0247 at 244.433 kb on - strand, within Dshi_0247 at 244.442 kb on + strand, within Dshi_0247 at 244.443 kb on + strand, within Dshi_0247 at 244.543 kb on - strand, within Dshi_0247 at 244.553 kb on + strand, within Dshi_0247 at 244.607 kb on - strand, within Dshi_0247 at 244.658 kb on - strand, within Dshi_0247 at 244.658 kb on - strand, within Dshi_0247 at 244.658 kb on - strand, within Dshi_0247 at 244.707 kb on - strand, within Dshi_0247 at 244.791 kb on + strand at 244.809 kb on - strand at 244.809 kb on - strand at 244.866 kb on - strand at 244.898 kb on + strand, within Dshi_0248 at 244.967 kb on - strand, within Dshi_0248 at 244.987 kb on - strand, within Dshi_0248 at 245.128 kb on + strand, within Dshi_0248 at 245.201 kb on - strand, within Dshi_0248 at 245.349 kb on + strand, within Dshi_0248 at 245.349 kb on + strand, within Dshi_0248 at 245.357 kb on - strand, within Dshi_0248 at 245.421 kb on - strand, within Dshi_0248 at 245.442 kb on + strand, within Dshi_0248 at 245.526 kb on + strand, within Dshi_0248 at 245.542 kb on + strand, within Dshi_0248 at 245.633 kb on + strand, within Dshi_0248 at 245.674 kb on + strand, within Dshi_0248 at 245.687 kb on - strand at 245.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 243,130 - +0.7 243,146 + -0.3 243,244 + Dshi_0245 0.17 +1.1 243,265 + Dshi_0245 0.22 -0.1 243,280 + Dshi_0245 0.25 +0.4 243,301 + Dshi_0245 0.29 +0.2 243,310 - Dshi_0245 0.30 +0.2 243,423 + Dshi_0245 0.53 -0.4 243,485 - Dshi_0245 0.65 +0.3 243,610 - Dshi_0245 0.89 +1.1 243,645 - +1.1 243,754 + +0.5 244,046 - -1.7 244,071 + +0.0 244,172 - Dshi_0247 0.13 +0.1 244,185 + Dshi_0247 0.14 -0.2 244,218 + Dshi_0247 0.19 +1.0 244,218 + Dshi_0247 0.19 +0.2 244,307 - Dshi_0247 0.32 -1.4 244,322 + Dshi_0247 0.34 -0.3 244,372 - Dshi_0247 0.41 +0.0 244,433 - Dshi_0247 0.50 -0.2 244,442 + Dshi_0247 0.51 +0.0 244,443 + Dshi_0247 0.51 +1.2 244,543 - Dshi_0247 0.66 +0.4 244,553 + Dshi_0247 0.67 -0.6 244,607 - Dshi_0247 0.75 -0.5 244,658 - Dshi_0247 0.82 -0.9 244,658 - Dshi_0247 0.82 -0.7 244,658 - Dshi_0247 0.82 +0.7 244,707 - Dshi_0247 0.89 -0.6 244,791 + +0.0 244,809 - +0.0 244,809 - -0.7 244,866 - -0.2 244,898 + Dshi_0248 0.11 +0.1 244,967 - Dshi_0248 0.18 +0.2 244,987 - Dshi_0248 0.20 +1.9 245,128 + Dshi_0248 0.34 +0.5 245,201 - Dshi_0248 0.41 +0.1 245,349 + Dshi_0248 0.56 -0.0 245,349 + Dshi_0248 0.56 +0.2 245,357 - Dshi_0248 0.57 -0.0 245,421 - Dshi_0248 0.64 +0.2 245,442 + Dshi_0248 0.66 -0.4 245,526 + Dshi_0248 0.74 +0.8 245,542 + Dshi_0248 0.76 +0.2 245,633 + Dshi_0248 0.85 +0.3 245,674 + Dshi_0248 0.89 +0.8 245,687 - -2.1 245,692 - +0.0
Or see this region's nucleotide sequence