Experiment: BG11 with nitrate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0474 and petN are separated by 15 nucleotides petN and clpS are separated by 7 nucleotides clpS and ylmG are separated by 31 nucleotides ylmG and lepB are separated by 30 nucleotides lepB and lepA overlap by 1 nucleotides
Synpcc7942_0474: Synpcc7942_0474 - hypothetical protein, at 460,296 to 460,433
_0474
Synpcc7942_0475: petN - cytochrome b6-f complex subunit VIII, at 460,449 to 460,544
petN
Synpcc7942_0476: clpS - ATP-dependent Clp protease adaptor, at 460,552 to 460,962
clpS
Synpcc7942_0477: ylmG - conserved hypothetical protein YCF19, at 460,994 to 461,293
ylmG
Synpcc7942_0478: lepB - thylakoidal processing peptidase. Serine peptidase. MEROPS family S26A, at 461,324 to 461,935
lepB
Synpcc7942_0479: lepA - GTP-binding protein LepA, at 461,935 to 463,749
lepA
Position (kb) 461
462 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 460.653 kb on + strand at 460.656 kb on - strand, within clpS at 460.656 kb on - strand, within clpS at 460.666 kb on - strand, within clpS at 460.671 kb on - strand, within clpS at 460.688 kb on + strand, within clpS at 460.690 kb on + strand, within clpS at 460.695 kb on + strand, within clpS at 460.703 kb on + strand, within clpS at 460.717 kb on + strand, within clpS at 460.717 kb on + strand, within clpS at 460.769 kb on - strand, within clpS at 460.863 kb on + strand, within clpS at 460.881 kb on - strand, within clpS at 460.883 kb on + strand, within clpS at 460.885 kb on + strand, within clpS at 460.891 kb on - strand, within clpS at 460.915 kb on - strand, within clpS at 460.925 kb on - strand at 460.961 kb on + strand at 460.964 kb on - strand at 461.046 kb on - strand, within ylmG at 461.046 kb on - strand, within ylmG at 461.059 kb on - strand, within ylmG at 461.110 kb on - strand, within ylmG at 461.165 kb on - strand, within ylmG at 461.288 kb on + strand at 461.296 kb on - strand at 461.298 kb on - strand at 461.335 kb on - strand at 461.338 kb on - strand at 461.362 kb on + strand at 461.363 kb on + strand at 461.363 kb on + strand at 461.371 kb on - strand at 461.371 kb on - strand at 461.372 kb on + strand at 461.378 kb on - strand at 461.380 kb on + strand at 461.902 kb on - strand at 461.931 kb on + strand at 461.951 kb on + strand at 461.951 kb on + strand at 461.963 kb on + strand at 461.963 kb on + strand at 461.973 kb on - strand at 461.983 kb on + strand at 461.988 kb on + strand at 462.097 kb on - strand at 462.155 kb on + strand, within lepA at 462.193 kb on + strand, within lepA at 462.203 kb on + strand, within lepA at 462.223 kb on - strand, within lepA at 462.271 kb on + strand, within lepA at 462.292 kb on - strand, within lepA at 462.334 kb on + strand, within lepA at 462.347 kb on - strand, within lepA at 462.347 kb on - strand, within lepA at 462.349 kb on - strand, within lepA at 462.363 kb on + strand, within lepA at 462.363 kb on + strand, within lepA at 462.371 kb on - strand, within lepA at 462.414 kb on - strand, within lepA at 462.527 kb on - strand, within lepA at 462.560 kb on - strand, within lepA at 462.560 kb on - strand, within lepA at 462.560 kb on - strand, within lepA at 462.576 kb on + strand, within lepA at 462.626 kb on + strand, within lepA at 462.667 kb on + strand, within lepA at 462.738 kb on - strand, within lepA at 462.788 kb on - strand, within lepA at 462.838 kb on - strand, within lepA at 462.842 kb on + strand, within lepA at 462.870 kb on - strand, within lepA at 462.893 kb on + strand, within lepA at 462.894 kb on + strand, within lepA at 462.928 kb on - strand, within lepA
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrate 50 mM remove 460,653 + +1.8 460,656 - clpS Synpcc7942_0476 0.25 +0.2 460,656 - clpS Synpcc7942_0476 0.25 +2.2 460,666 - clpS Synpcc7942_0476 0.28 +0.6 460,671 - clpS Synpcc7942_0476 0.29 +0.8 460,688 + clpS Synpcc7942_0476 0.33 -1.4 460,690 + clpS Synpcc7942_0476 0.34 -1.9 460,695 + clpS Synpcc7942_0476 0.35 -0.6 460,703 + clpS Synpcc7942_0476 0.37 +0.4 460,717 + clpS Synpcc7942_0476 0.40 -0.7 460,717 + clpS Synpcc7942_0476 0.40 +0.9 460,769 - clpS Synpcc7942_0476 0.53 -0.8 460,863 + clpS Synpcc7942_0476 0.76 -1.6 460,881 - clpS Synpcc7942_0476 0.80 +0.9 460,883 + clpS Synpcc7942_0476 0.81 -1.4 460,885 + clpS Synpcc7942_0476 0.81 -1.4 460,891 - clpS Synpcc7942_0476 0.82 -0.9 460,915 - clpS Synpcc7942_0476 0.88 +0.2 460,925 - -0.7 460,961 + +1.5 460,964 - +6.2 461,046 - ylmG Synpcc7942_0477 0.17 -0.8 461,046 - ylmG Synpcc7942_0477 0.17 -1.4 461,059 - ylmG Synpcc7942_0477 0.22 -1.0 461,110 - ylmG Synpcc7942_0477 0.39 -1.4 461,165 - ylmG Synpcc7942_0477 0.57 +2.2 461,288 + -1.3 461,296 - +0.6 461,298 - -1.0 461,335 - -1.8 461,338 - -1.5 461,362 + -1.9 461,363 + +1.2 461,363 + +1.5 461,371 - +0.6 461,371 - +0.6 461,372 + +0.7 461,378 - -1.5 461,380 + -1.7 461,902 - +0.0 461,931 + -0.5 461,951 + +1.9 461,951 + +1.7 461,963 + +0.6 461,963 + +0.0 461,973 - +0.7 461,983 + +2.6 461,988 + -0.2 462,097 - +1.4 462,155 + lepA Synpcc7942_0479 0.12 +1.2 462,193 + lepA Synpcc7942_0479 0.14 -0.1 462,203 + lepA Synpcc7942_0479 0.15 +1.2 462,223 - lepA Synpcc7942_0479 0.16 +0.3 462,271 + lepA Synpcc7942_0479 0.19 +1.2 462,292 - lepA Synpcc7942_0479 0.20 +4.0 462,334 + lepA Synpcc7942_0479 0.22 +1.9 462,347 - lepA Synpcc7942_0479 0.23 +2.2 462,347 - lepA Synpcc7942_0479 0.23 +1.0 462,349 - lepA Synpcc7942_0479 0.23 +3.1 462,363 + lepA Synpcc7942_0479 0.24 +1.2 462,363 + lepA Synpcc7942_0479 0.24 +0.8 462,371 - lepA Synpcc7942_0479 0.24 +0.3 462,414 - lepA Synpcc7942_0479 0.26 +1.1 462,527 - lepA Synpcc7942_0479 0.33 +0.9 462,560 - lepA Synpcc7942_0479 0.34 +1.9 462,560 - lepA Synpcc7942_0479 0.34 +1.4 462,560 - lepA Synpcc7942_0479 0.34 +3.1 462,576 + lepA Synpcc7942_0479 0.35 +3.0 462,626 + lepA Synpcc7942_0479 0.38 +1.0 462,667 + lepA Synpcc7942_0479 0.40 +2.5 462,738 - lepA Synpcc7942_0479 0.44 +1.9 462,788 - lepA Synpcc7942_0479 0.47 +0.9 462,838 - lepA Synpcc7942_0479 0.50 +3.7 462,842 + lepA Synpcc7942_0479 0.50 +1.5 462,870 - lepA Synpcc7942_0479 0.52 +0.9 462,893 + lepA Synpcc7942_0479 0.53 +2.4 462,894 + lepA Synpcc7942_0479 0.53 +1.8 462,928 - lepA Synpcc7942_0479 0.55 +1.0
Or see this region's nucleotide sequence