Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_97

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_96 and Pf1N1B4_97 overlap by 1 nucleotidesPf1N1B4_97 and Pf1N1B4_98 are separated by 535 nucleotides Pf1N1B4_96 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 100,858 to 101,961 _96 Pf1N1B4_97 - Selenide,water dikinase (EC 2.7.9.3), at 101,961 to 102,995 _97 Pf1N1B4_98 - Nucleoside-specific channel-forming protein Tsx precursor, at 103,531 to 104,487 _98 Position (kb) 101 102 103Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 100.971 kb on - strand, within Pf1N1B4_96at 100.971 kb on - strand, within Pf1N1B4_96at 100.973 kb on + strand, within Pf1N1B4_96at 101.160 kb on - strand, within Pf1N1B4_96at 101.195 kb on + strand, within Pf1N1B4_96at 101.196 kb on - strand, within Pf1N1B4_96at 101.341 kb on - strand, within Pf1N1B4_96at 101.682 kb on - strand, within Pf1N1B4_96at 101.940 kb on - strandat 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.157 kb on + strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.158 kb on - strand, within Pf1N1B4_97at 102.209 kb on - strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.211 kb on + strand, within Pf1N1B4_97at 102.212 kb on - strandat 102.212 kb on - strand, within Pf1N1B4_97at 102.212 kb on - strand, within Pf1N1B4_97at 102.212 kb on - strand, within Pf1N1B4_97at 102.212 kb on - strandat 102.212 kb on - strand, within Pf1N1B4_97at 102.475 kb on + strand, within Pf1N1B4_97at 102.475 kb on + strand, within Pf1N1B4_97at 102.476 kb on - strand, within Pf1N1B4_97at 102.476 kb on - strand, within Pf1N1B4_97at 102.722 kb on - strand, within Pf1N1B4_97at 102.722 kb on - strand, within Pf1N1B4_97at 102.722 kb on - strand, within Pf1N1B4_97at 102.835 kb on + strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 102.836 kb on - strand, within Pf1N1B4_97at 103.032 kb on + strandat 103.033 kb on - strandat 103.076 kb on - strandat 103.225 kb on - strandat 103.379 kb on - strandat 103.398 kb on - strandat 103.529 kb on + strandat 103.705 kb on + strand, within Pf1N1B4_98at 103.789 kb on + strand, within Pf1N1B4_98at 103.798 kb on + strand, within Pf1N1B4_98at 103.798 kb on + strand, within Pf1N1B4_98at 103.799 kb on - strand, within Pf1N1B4_98at 103.804 kb on + strand, within Pf1N1B4_98at 103.804 kb on + strand, within Pf1N1B4_98at 103.804 kb on + strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strandat 103.834 kb on + strand, within Pf1N1B4_98at 103.993 kb on + strand, within Pf1N1B4_98at 103.993 kb on + strand, within Pf1N1B4_98at 103.993 kb on + strandat 103.993 kb on + strand, within Pf1N1B4_98at 103.994 kb on - strand, within Pf1N1B4_98at 103.994 kb on - strand, within Pf1N1B4_98

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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100,971 - Pf1N1B4_96 0.10 -2.0
100,971 - Pf1N1B4_96 0.10 -0.0
100,973 + Pf1N1B4_96 0.10 -2.1
101,160 - Pf1N1B4_96 0.27 -0.0
101,195 + Pf1N1B4_96 0.31 -1.6
101,196 - Pf1N1B4_96 0.31 -4.1
101,341 - Pf1N1B4_96 0.44 -0.0
101,682 - Pf1N1B4_96 0.75 -2.5
101,940 - -3.5
102,157 + Pf1N1B4_97 0.19 -0.0
102,157 + Pf1N1B4_97 0.19 -0.0
102,157 + Pf1N1B4_97 0.19 -4.0
102,157 + Pf1N1B4_97 0.19 -0.0
102,157 + Pf1N1B4_97 0.19 -2.2
102,158 - Pf1N1B4_97 0.19 -0.0
102,158 - Pf1N1B4_97 0.19 -0.0
102,158 - Pf1N1B4_97 0.19 -4.0
102,158 - Pf1N1B4_97 0.19 -0.0
102,209 - Pf1N1B4_97 0.24 -3.6
102,211 + Pf1N1B4_97 0.24 -0.0
102,211 + Pf1N1B4_97 0.24 -0.0
102,211 + Pf1N1B4_97 0.24 -0.0
102,211 + Pf1N1B4_97 0.24 -5.3
102,212 - -0.0
102,212 - Pf1N1B4_97 0.24 -3.1
102,212 - Pf1N1B4_97 0.24 -0.0
102,212 - Pf1N1B4_97 0.24 -0.0
102,212 - -1.7
102,212 - Pf1N1B4_97 0.24 -0.0
102,475 + Pf1N1B4_97 0.50 -0.0
102,475 + Pf1N1B4_97 0.50 -2.9
102,476 - Pf1N1B4_97 0.50 -3.8
102,476 - Pf1N1B4_97 0.50 -0.0
102,722 - Pf1N1B4_97 0.74 -0.0
102,722 - Pf1N1B4_97 0.74 -0.0
102,722 - Pf1N1B4_97 0.74 -0.0
102,835 + Pf1N1B4_97 0.84 -3.6
102,836 - Pf1N1B4_97 0.85 -0.0
102,836 - Pf1N1B4_97 0.85 -0.0
102,836 - Pf1N1B4_97 0.85 -0.0
102,836 - Pf1N1B4_97 0.85 -3.0
103,032 + -0.0
103,033 - -0.0
103,076 - -1.8
103,225 - -1.9
103,379 - -0.0
103,398 - -0.0
103,529 + -0.0
103,705 + Pf1N1B4_98 0.18 -0.0
103,789 + Pf1N1B4_98 0.27 -1.3
103,798 + Pf1N1B4_98 0.28 -0.0
103,798 + Pf1N1B4_98 0.28 -0.0
103,799 - Pf1N1B4_98 0.28 +1.0
103,804 + Pf1N1B4_98 0.29 -2.1
103,804 + Pf1N1B4_98 0.29 -2.4
103,804 + Pf1N1B4_98 0.29 -2.8
103,805 - Pf1N1B4_98 0.29 -0.0
103,805 - Pf1N1B4_98 0.29 -1.6
103,805 - Pf1N1B4_98 0.29 -2.2
103,805 - Pf1N1B4_98 0.29 -0.0
103,805 - -1.8
103,834 + Pf1N1B4_98 0.32 -2.5
103,993 + Pf1N1B4_98 0.48 -0.0
103,993 + Pf1N1B4_98 0.48 -1.8
103,993 + -0.4
103,993 + Pf1N1B4_98 0.48 -2.6
103,994 - Pf1N1B4_98 0.48 -1.3
103,994 - Pf1N1B4_98 0.48 -0.0

Or see this region's nucleotide sequence