Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_96 and Pf1N1B4_97 overlap by 1 nucleotides Pf1N1B4_97 and Pf1N1B4_98 are separated by 535 nucleotides
Pf1N1B4_96 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 100,858 to 101,961
_96
Pf1N1B4_97 - Selenide,water dikinase (EC 2.7.9.3), at 101,961 to 102,995
_97
Pf1N1B4_98 - Nucleoside-specific channel-forming protein Tsx precursor, at 103,531 to 104,487
_98
Position (kb)
101
102
103 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 100.971 kb on - strand, within Pf1N1B4_96 at 100.971 kb on - strand, within Pf1N1B4_96 at 100.973 kb on + strand, within Pf1N1B4_96 at 101.160 kb on - strand, within Pf1N1B4_96 at 101.195 kb on + strand, within Pf1N1B4_96 at 101.196 kb on - strand, within Pf1N1B4_96 at 101.341 kb on - strand, within Pf1N1B4_96 at 101.682 kb on - strand, within Pf1N1B4_96 at 101.940 kb on - strand at 102.157 kb on + strand, within Pf1N1B4_97 at 102.157 kb on + strand, within Pf1N1B4_97 at 102.157 kb on + strand, within Pf1N1B4_97 at 102.157 kb on + strand, within Pf1N1B4_97 at 102.157 kb on + strand, within Pf1N1B4_97 at 102.158 kb on - strand, within Pf1N1B4_97 at 102.158 kb on - strand, within Pf1N1B4_97 at 102.158 kb on - strand, within Pf1N1B4_97 at 102.158 kb on - strand, within Pf1N1B4_97 at 102.209 kb on - strand, within Pf1N1B4_97 at 102.211 kb on + strand, within Pf1N1B4_97 at 102.211 kb on + strand, within Pf1N1B4_97 at 102.211 kb on + strand, within Pf1N1B4_97 at 102.211 kb on + strand, within Pf1N1B4_97 at 102.212 kb on - strand at 102.212 kb on - strand, within Pf1N1B4_97 at 102.212 kb on - strand, within Pf1N1B4_97 at 102.212 kb on - strand, within Pf1N1B4_97 at 102.212 kb on - strand at 102.212 kb on - strand, within Pf1N1B4_97 at 102.475 kb on + strand, within Pf1N1B4_97 at 102.475 kb on + strand, within Pf1N1B4_97 at 102.476 kb on - strand, within Pf1N1B4_97 at 102.476 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.835 kb on + strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 103.032 kb on + strand at 103.033 kb on - strand at 103.076 kb on - strand at 103.225 kb on - strand at 103.379 kb on - strand at 103.398 kb on - strand at 103.529 kb on + strand at 103.705 kb on + strand, within Pf1N1B4_98 at 103.789 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.799 kb on - strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand at 103.834 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand at 103.993 kb on + strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 100,971 - Pf1N1B4_96 0.10 -2.0 100,971 - Pf1N1B4_96 0.10 -0.0 100,973 + Pf1N1B4_96 0.10 -2.1 101,160 - Pf1N1B4_96 0.27 -0.0 101,195 + Pf1N1B4_96 0.31 -1.6 101,196 - Pf1N1B4_96 0.31 -4.1 101,341 - Pf1N1B4_96 0.44 -0.0 101,682 - Pf1N1B4_96 0.75 -2.5 101,940 - -3.5 102,157 + Pf1N1B4_97 0.19 -0.0 102,157 + Pf1N1B4_97 0.19 -0.0 102,157 + Pf1N1B4_97 0.19 -4.0 102,157 + Pf1N1B4_97 0.19 -0.0 102,157 + Pf1N1B4_97 0.19 -2.2 102,158 - Pf1N1B4_97 0.19 -0.0 102,158 - Pf1N1B4_97 0.19 -0.0 102,158 - Pf1N1B4_97 0.19 -4.0 102,158 - Pf1N1B4_97 0.19 -0.0 102,209 - Pf1N1B4_97 0.24 -3.6 102,211 + Pf1N1B4_97 0.24 -0.0 102,211 + Pf1N1B4_97 0.24 -0.0 102,211 + Pf1N1B4_97 0.24 -0.0 102,211 + Pf1N1B4_97 0.24 -5.3 102,212 - -0.0 102,212 - Pf1N1B4_97 0.24 -3.1 102,212 - Pf1N1B4_97 0.24 -0.0 102,212 - Pf1N1B4_97 0.24 -0.0 102,212 - -1.7 102,212 - Pf1N1B4_97 0.24 -0.0 102,475 + Pf1N1B4_97 0.50 -0.0 102,475 + Pf1N1B4_97 0.50 -2.9 102,476 - Pf1N1B4_97 0.50 -3.8 102,476 - Pf1N1B4_97 0.50 -0.0 102,722 - Pf1N1B4_97 0.74 -0.0 102,722 - Pf1N1B4_97 0.74 -0.0 102,722 - Pf1N1B4_97 0.74 -0.0 102,835 + Pf1N1B4_97 0.84 -3.6 102,836 - Pf1N1B4_97 0.85 -0.0 102,836 - Pf1N1B4_97 0.85 -0.0 102,836 - Pf1N1B4_97 0.85 -0.0 102,836 - Pf1N1B4_97 0.85 -3.0 103,032 + -0.0 103,033 - -0.0 103,076 - -1.8 103,225 - -1.9 103,379 - -0.0 103,398 - -0.0 103,529 + -0.0 103,705 + Pf1N1B4_98 0.18 -0.0 103,789 + Pf1N1B4_98 0.27 -1.3 103,798 + Pf1N1B4_98 0.28 -0.0 103,798 + Pf1N1B4_98 0.28 -0.0 103,799 - Pf1N1B4_98 0.28 +1.0 103,804 + Pf1N1B4_98 0.29 -2.1 103,804 + Pf1N1B4_98 0.29 -2.4 103,804 + Pf1N1B4_98 0.29 -2.8 103,805 - Pf1N1B4_98 0.29 -0.0 103,805 - Pf1N1B4_98 0.29 -1.6 103,805 - Pf1N1B4_98 0.29 -2.2 103,805 - Pf1N1B4_98 0.29 -0.0 103,805 - -1.8 103,834 + Pf1N1B4_98 0.32 -2.5 103,993 + Pf1N1B4_98 0.48 -0.0 103,993 + Pf1N1B4_98 0.48 -1.8 103,993 + -0.4 103,993 + Pf1N1B4_98 0.48 -2.6 103,994 - Pf1N1B4_98 0.48 -1.3 103,994 - Pf1N1B4_98 0.48 -0.0
Or see this region's nucleotide sequence