Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_847

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_845 and Pf1N1B4_846 are separated by 232 nucleotidesPf1N1B4_846 and Pf1N1B4_847 are separated by 15 nucleotidesPf1N1B4_847 and Pf1N1B4_848 are separated by 164 nucleotidesPf1N1B4_848 and Pf1N1B4_849 overlap by 13 nucleotides Pf1N1B4_845 - Membrane proteins related to metalloendopeptidases, at 973,712 to 974,533 _845 Pf1N1B4_846 - hypothetical protein, at 974,766 to 974,969 _846 Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664 _847 Pf1N1B4_848 - Aliphatic amidase AmiE (EC 3.5.1.4), at 976,829 to 977,620 _848 Pf1N1B4_849 - Aspartate aminotransferase (EC 2.6.1.1), at 977,608 to 978,756 _849 Position (kb) 974 975 976 977Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 974.003 kb on + strand, within Pf1N1B4_845at 974.215 kb on + strand, within Pf1N1B4_845at 974.215 kb on + strand, within Pf1N1B4_845at 974.215 kb on + strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.216 kb on - strand, within Pf1N1B4_845at 974.304 kb on - strand, within Pf1N1B4_845at 974.304 kb on - strand, within Pf1N1B4_845at 974.304 kb on - strand, within Pf1N1B4_845at 974.368 kb on + strand, within Pf1N1B4_845at 974.368 kb on + strand, within Pf1N1B4_845at 974.368 kb on + strand, within Pf1N1B4_845at 974.369 kb on - strand, within Pf1N1B4_845at 974.369 kb on - strand, within Pf1N1B4_845at 974.461 kb on + strandat 974.461 kb on + strandat 974.462 kb on - strandat 974.462 kb on - strandat 974.656 kb on - strandat 974.656 kb on - strandat 974.729 kb on + strandat 974.915 kb on + strand, within Pf1N1B4_846at 974.915 kb on + strandat 974.916 kb on - strandat 974.922 kb on + strand, within Pf1N1B4_846at 974.972 kb on + strandat 974.972 kb on + strandat 975.016 kb on - strandat 975.016 kb on - strandat 975.246 kb on - strand, within Pf1N1B4_847at 975.246 kb on - strand, within Pf1N1B4_847at 975.326 kb on + strand, within Pf1N1B4_847at 975.327 kb on - strand, within Pf1N1B4_847at 975.327 kb on - strandat 975.327 kb on - strand, within Pf1N1B4_847at 975.372 kb on + strand, within Pf1N1B4_847at 975.372 kb on + strand, within Pf1N1B4_847at 975.373 kb on - strand, within Pf1N1B4_847at 975.373 kb on - strand, within Pf1N1B4_847at 975.373 kb on - strandat 975.654 kb on + strand, within Pf1N1B4_847at 975.654 kb on + strand, within Pf1N1B4_847at 975.654 kb on + strand, within Pf1N1B4_847at 975.654 kb on + strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strand, within Pf1N1B4_847at 975.655 kb on - strandat 975.861 kb on + strand, within Pf1N1B4_847at 975.861 kb on + strand, within Pf1N1B4_847at 975.862 kb on - strand, within Pf1N1B4_847at 975.862 kb on - strand, within Pf1N1B4_847at 975.862 kb on - strandat 975.862 kb on - strand, within Pf1N1B4_847at 975.963 kb on + strand, within Pf1N1B4_847at 976.065 kb on + strand, within Pf1N1B4_847at 976.065 kb on + strand, within Pf1N1B4_847at 976.065 kb on + strand, within Pf1N1B4_847at 976.066 kb on - strand, within Pf1N1B4_847at 976.066 kb on - strand, within Pf1N1B4_847at 976.101 kb on + strand, within Pf1N1B4_847at 976.101 kb on + strand, within Pf1N1B4_847at 976.102 kb on - strand, within Pf1N1B4_847at 976.152 kb on + strandat 976.152 kb on + strand, within Pf1N1B4_847at 976.153 kb on - strand, within Pf1N1B4_847at 976.170 kb on + strand, within Pf1N1B4_847at 976.290 kb on + strand, within Pf1N1B4_847at 976.290 kb on + strand, within Pf1N1B4_847at 976.291 kb on - strand, within Pf1N1B4_847at 976.479 kb on + strand, within Pf1N1B4_847at 976.479 kb on + strand, within Pf1N1B4_847at 976.479 kb on + strand, within Pf1N1B4_847at 976.480 kb on - strand, within Pf1N1B4_847at 976.527 kb on + strandat 976.527 kb on + strandat 976.527 kb on + strandat 976.528 kb on - strandat 976.528 kb on - strandat 976.528 kb on - strandat 976.528 kb on - strandat 976.528 kb on - strandat 976.543 kb on + strandat 976.988 kb on - strand, within Pf1N1B4_848at 976.989 kb on + strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 977.037 kb on + strand, within Pf1N1B4_848at 977.037 kb on + strandat 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.116 kb on - strand, within Pf1N1B4_848at 977.173 kb on - strand, within Pf1N1B4_848at 977.283 kb on + strand, within Pf1N1B4_848at 977.284 kb on - strand, within Pf1N1B4_848at 977.410 kb on + strand, within Pf1N1B4_848at 977.410 kb on + strand, within Pf1N1B4_848at 977.411 kb on - strand, within Pf1N1B4_848at 977.620 kb on + strandat 977.621 kb on - strandat 977.621 kb on - strandat 977.621 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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974,003 + Pf1N1B4_845 0.35 -0.0
974,215 + Pf1N1B4_845 0.61 -2.6
974,215 + Pf1N1B4_845 0.61 -0.0
974,215 + Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 -2.5
974,216 - Pf1N1B4_845 0.61 -0.0
974,216 - Pf1N1B4_845 0.61 -2.7
974,216 - Pf1N1B4_845 0.61 -0.0
974,304 - Pf1N1B4_845 0.72 -2.4
974,304 - Pf1N1B4_845 0.72 -0.0
974,304 - Pf1N1B4_845 0.72 -2.6
974,368 + Pf1N1B4_845 0.80 -0.0
974,368 + Pf1N1B4_845 0.80 -0.0
974,368 + Pf1N1B4_845 0.80 -1.3
974,369 - Pf1N1B4_845 0.80 -0.0
974,369 - Pf1N1B4_845 0.80 -0.0
974,461 + -0.0
974,461 + -0.0
974,462 - -0.0
974,462 - -0.0
974,656 - -1.3
974,656 - -0.0
974,729 + -0.0
974,915 + Pf1N1B4_846 0.73 -2.2
974,915 + -0.0
974,916 - -4.5
974,922 + Pf1N1B4_846 0.76 -1.3
974,972 + -0.0
974,972 + -4.3
975,016 - -4.4
975,016 - -0.0
975,246 - Pf1N1B4_847 0.16 -0.0
975,246 - Pf1N1B4_847 0.16 -5.0
975,326 + Pf1N1B4_847 0.20 -0.0
975,327 - Pf1N1B4_847 0.20 -0.0
975,327 - -0.0
975,327 - Pf1N1B4_847 0.20 -0.0
975,372 + Pf1N1B4_847 0.23 -0.0
975,372 + Pf1N1B4_847 0.23 -0.0
975,373 - Pf1N1B4_847 0.23 -0.0
975,373 - Pf1N1B4_847 0.23 -3.1
975,373 - -4.6
975,654 + Pf1N1B4_847 0.40 -0.0
975,654 + Pf1N1B4_847 0.40 -3.4
975,654 + Pf1N1B4_847 0.40 -0.0
975,654 + Pf1N1B4_847 0.40 -0.0
975,655 - Pf1N1B4_847 0.40 -4.7
975,655 - Pf1N1B4_847 0.40 -0.0
975,655 - Pf1N1B4_847 0.40 -3.1
975,655 - Pf1N1B4_847 0.40 -0.0
975,655 - Pf1N1B4_847 0.40 -0.0
975,655 - -5.1
975,861 + Pf1N1B4_847 0.52 -0.0
975,861 + Pf1N1B4_847 0.52 -0.0
975,862 - Pf1N1B4_847 0.52 -0.0
975,862 - Pf1N1B4_847 0.52 -0.0
975,862 - -0.0
975,862 - Pf1N1B4_847 0.52 -4.0
975,963 + Pf1N1B4_847 0.58 -0.0
976,065 + Pf1N1B4_847 0.64 -0.0
976,065 + Pf1N1B4_847 0.64 -0.0
976,065 + Pf1N1B4_847 0.64 -3.5
976,066 - Pf1N1B4_847 0.64 -0.0
976,066 - Pf1N1B4_847 0.64 -1.0
976,101 + Pf1N1B4_847 0.66 -6.2
976,101 + Pf1N1B4_847 0.66 -0.0
976,102 - Pf1N1B4_847 0.66 -0.0
976,152 + -0.0
976,152 + Pf1N1B4_847 0.69 -0.0
976,153 - Pf1N1B4_847 0.70 -0.0
976,170 + Pf1N1B4_847 0.71 -0.0
976,290 + Pf1N1B4_847 0.78 -0.0
976,290 + Pf1N1B4_847 0.78 -0.0
976,291 - Pf1N1B4_847 0.78 -0.0
976,479 + Pf1N1B4_847 0.89 -0.0
976,479 + Pf1N1B4_847 0.89 -0.0
976,479 + Pf1N1B4_847 0.89 -3.5
976,480 - Pf1N1B4_847 0.89 -0.8
976,527 + -0.0
976,527 + -0.0
976,527 + -0.0
976,528 - -0.0
976,528 - -0.0
976,528 - -4.3
976,528 - -0.0
976,528 - -0.0
976,543 + -2.5
976,988 - Pf1N1B4_848 0.20 -2.0
976,989 + Pf1N1B4_848 0.20 -0.0
976,990 - Pf1N1B4_848 0.20 -1.2
976,990 - Pf1N1B4_848 0.20 -0.0
976,990 - Pf1N1B4_848 0.20 -0.0
976,990 - Pf1N1B4_848 0.20 -0.0
977,037 + Pf1N1B4_848 0.26 -1.1
977,037 + -2.6
977,038 - Pf1N1B4_848 0.26 -0.0
977,038 - Pf1N1B4_848 0.26 -2.9
977,038 - Pf1N1B4_848 0.26 -1.4
977,038 - Pf1N1B4_848 0.26 -2.3
977,038 - Pf1N1B4_848 0.26 -2.0
977,116 - Pf1N1B4_848 0.36 -0.0
977,173 - Pf1N1B4_848 0.43 -0.0
977,283 + Pf1N1B4_848 0.57 -0.0
977,284 - Pf1N1B4_848 0.57 -1.5
977,410 + Pf1N1B4_848 0.73 -0.0
977,410 + Pf1N1B4_848 0.73 -0.0
977,411 - Pf1N1B4_848 0.73 -0.0
977,620 + -0.0
977,621 - -3.5
977,621 - -1.9
977,621 - -0.0

Or see this region's nucleotide sequence