Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_777

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_776 and Pf1N1B4_777 overlap by 1 nucleotidesPf1N1B4_777 and Pf1N1B4_778 are separated by 118 nucleotides Pf1N1B4_776 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 880,987 to 882,315 _776 Pf1N1B4_777 - Membrane protein glpM, at 882,315 to 882,653 _777 Pf1N1B4_778 - hypothetical protein, at 882,772 to 883,662 _778 Position (kb) 882 883Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 881.382 kb on - strand, within Pf1N1B4_776at 881.782 kb on - strand, within Pf1N1B4_776at 881.782 kb on - strand, within Pf1N1B4_776at 881.896 kb on + strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.915 kb on + strand, within Pf1N1B4_776at 882.298 kb on + strandat 882.299 kb on - strandat 882.355 kb on + strand, within Pf1N1B4_777at 882.390 kb on + strand, within Pf1N1B4_777at 882.391 kb on - strand, within Pf1N1B4_777at 882.391 kb on - strand, within Pf1N1B4_777at 882.391 kb on - strand, within Pf1N1B4_777at 882.393 kb on + strand, within Pf1N1B4_777at 882.393 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.529 kb on - strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strandat 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.745 kb on - strandat 882.752 kb on + strandat 882.752 kb on + strandat 882.753 kb on - strandat 882.872 kb on + strand, within Pf1N1B4_778at 882.872 kb on + strand, within Pf1N1B4_778at 882.872 kb on + strand, within Pf1N1B4_778at 882.872 kb on + strand, within Pf1N1B4_778at 882.873 kb on - strandat 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.876 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.933 kb on - strand, within Pf1N1B4_778at 883.210 kb on + strandat 883.462 kb on - strand, within Pf1N1B4_778at 883.462 kb on - strand, within Pf1N1B4_778at 883.462 kb on - strand, within Pf1N1B4_778at 883.571 kb on + strand, within Pf1N1B4_778at 883.571 kb on + strandat 883.571 kb on + strand, within Pf1N1B4_778at 883.572 kb on - strand, within Pf1N1B4_778

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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881,382 - Pf1N1B4_776 0.30 -1.6
881,782 - Pf1N1B4_776 0.60 -0.1
881,782 - Pf1N1B4_776 0.60 -1.5
881,896 + Pf1N1B4_776 0.68 -0.1
881,897 - Pf1N1B4_776 0.68 -3.0
881,897 - Pf1N1B4_776 0.68 -2.6
881,897 - Pf1N1B4_776 0.68 -0.1
881,897 - Pf1N1B4_776 0.68 -0.1
881,897 - Pf1N1B4_776 0.68 -0.1
881,915 + Pf1N1B4_776 0.70 -2.0
882,298 + -0.1
882,299 - -2.7
882,355 + Pf1N1B4_777 0.12 -2.6
882,390 + Pf1N1B4_777 0.22 -2.0
882,391 - Pf1N1B4_777 0.22 -4.4
882,391 - Pf1N1B4_777 0.22 -3.4
882,391 - Pf1N1B4_777 0.22 -3.6
882,393 + Pf1N1B4_777 0.23 -0.1
882,393 + Pf1N1B4_777 0.23 -0.1
882,444 + Pf1N1B4_777 0.38 -0.4
882,444 + Pf1N1B4_777 0.38 -2.5
882,444 + Pf1N1B4_777 0.38 -0.1
882,444 + Pf1N1B4_777 0.38 -3.4
882,444 + Pf1N1B4_777 0.38 -0.1
882,444 + Pf1N1B4_777 0.38 -0.1
882,444 + Pf1N1B4_777 0.38 -0.1
882,444 + Pf1N1B4_777 0.38 -0.2
882,444 + Pf1N1B4_777 0.38 -3.9
882,444 + Pf1N1B4_777 0.38 -2.6
882,444 + Pf1N1B4_777 0.38 -3.1
882,445 - Pf1N1B4_777 0.38 -0.1
882,445 - Pf1N1B4_777 0.38 -0.1
882,445 - Pf1N1B4_777 0.38 -0.1
882,445 - Pf1N1B4_777 0.38 -0.1
882,445 - Pf1N1B4_777 0.38 -0.1
882,519 + Pf1N1B4_777 0.60 -0.1
882,519 + Pf1N1B4_777 0.60 -2.3
882,519 + Pf1N1B4_777 0.60 -0.1
882,519 + Pf1N1B4_777 0.60 -0.1
882,519 + Pf1N1B4_777 0.60 -0.1
882,529 - Pf1N1B4_777 0.63 -0.1
882,543 + Pf1N1B4_777 0.67 -0.1
882,543 + Pf1N1B4_777 0.67 -0.1
882,543 + Pf1N1B4_777 0.67 -3.1
882,543 + Pf1N1B4_777 0.67 -0.1
882,543 + Pf1N1B4_777 0.67 -0.1
882,543 + Pf1N1B4_777 0.67 -3.5
882,543 + Pf1N1B4_777 0.67 -2.9
882,543 + Pf1N1B4_777 0.67 -0.1
882,543 + Pf1N1B4_777 0.67 -2.9
882,544 - Pf1N1B4_777 0.68 -3.1
882,544 - Pf1N1B4_777 0.68 -0.1
882,544 - Pf1N1B4_777 0.68 -0.1
882,544 - -0.1
882,544 - Pf1N1B4_777 0.68 -0.1
882,544 - Pf1N1B4_777 0.68 -0.1
882,544 - Pf1N1B4_777 0.68 -0.1
882,544 - Pf1N1B4_777 0.68 -0.1
882,544 - Pf1N1B4_777 0.68 -3.5
882,544 - Pf1N1B4_777 0.68 -4.8
882,745 - -1.6
882,752 + -1.3
882,752 + -1.6
882,753 - -0.1
882,872 + Pf1N1B4_778 0.11 -0.1
882,872 + Pf1N1B4_778 0.11 -0.1
882,872 + Pf1N1B4_778 0.11 -0.1
882,872 + Pf1N1B4_778 0.11 -0.1
882,873 - -1.7
882,873 - Pf1N1B4_778 0.11 -0.1
882,873 - Pf1N1B4_778 0.11 -0.1
882,873 - Pf1N1B4_778 0.11 -2.0
882,873 - Pf1N1B4_778 0.11 -0.1
882,873 - Pf1N1B4_778 0.11 -0.1
882,873 - Pf1N1B4_778 0.11 -0.9
882,876 + Pf1N1B4_778 0.12 -0.1
882,932 + Pf1N1B4_778 0.18 -0.1
882,932 + Pf1N1B4_778 0.18 -2.7
882,932 + Pf1N1B4_778 0.18 -0.1
882,932 + Pf1N1B4_778 0.18 -0.1
882,932 + Pf1N1B4_778 0.18 -2.6
882,933 - Pf1N1B4_778 0.18 -0.1
883,210 + -1.7
883,462 - Pf1N1B4_778 0.77 -0.1
883,462 - Pf1N1B4_778 0.77 -0.1
883,462 - Pf1N1B4_778 0.77 -0.1
883,571 + Pf1N1B4_778 0.90 -2.6
883,571 + -3.3
883,571 + Pf1N1B4_778 0.90 -1.2
883,572 - Pf1N1B4_778 0.90 -0.9

Or see this region's nucleotide sequence