Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_616

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_615 and Pf1N1B4_616 are separated by 69 nucleotidesPf1N1B4_616 and Pf1N1B4_617 are separated by 191 nucleotides Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472 _615 Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552 _616 Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673 _617 Position (kb) 712 713 714Strain fitness (log2 ratio) -3 -2 -1 0 1at 711.756 kb on + strand, within Pf1N1B4_615at 711.757 kb on - strand, within Pf1N1B4_615at 712.344 kb on + strandat 712.344 kb on + strand, within Pf1N1B4_615at 712.345 kb on - strand, within Pf1N1B4_615at 712.345 kb on - strand, within Pf1N1B4_615at 712.345 kb on - strand, within Pf1N1B4_615at 712.467 kb on + strandat 712.468 kb on - strandat 712.608 kb on + strandat 712.609 kb on - strandat 712.647 kb on + strand, within Pf1N1B4_616at 712.648 kb on - strand, within Pf1N1B4_616at 712.648 kb on - strand, within Pf1N1B4_616at 712.770 kb on + strand, within Pf1N1B4_616at 712.770 kb on + strand, within Pf1N1B4_616at 712.771 kb on - strand, within Pf1N1B4_616at 712.771 kb on - strand, within Pf1N1B4_616at 713.550 kb on + strandat 713.575 kb on + strandat 713.576 kb on - strandat 713.744 kb on - strandat 713.933 kb on + strand, within Pf1N1B4_617at 713.996 kb on + strandat 714.126 kb on - strand, within Pf1N1B4_617at 714.126 kb on - strandat 714.315 kb on - strand, within Pf1N1B4_617at 714.315 kb on - strand, within Pf1N1B4_617

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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711,756 + Pf1N1B4_615 0.43 -0.1
711,757 - Pf1N1B4_615 0.43 -0.1
712,344 + -2.3
712,344 + Pf1N1B4_615 0.90 -0.1
712,345 - Pf1N1B4_615 0.90 -0.1
712,345 - Pf1N1B4_615 0.90 -1.7
712,345 - Pf1N1B4_615 0.90 -0.1
712,467 + -2.2
712,468 - -0.1
712,608 + -0.1
712,609 - -0.1
712,647 + Pf1N1B4_616 0.10 -2.4
712,648 - Pf1N1B4_616 0.10 -0.1
712,648 - Pf1N1B4_616 0.10 -0.1
712,770 + Pf1N1B4_616 0.23 -3.2
712,770 + Pf1N1B4_616 0.23 -0.1
712,771 - Pf1N1B4_616 0.23 -2.6
712,771 - Pf1N1B4_616 0.23 -0.1
713,550 + -1.6
713,575 + -0.1
713,576 - -1.4
713,744 - -0.1
713,933 + Pf1N1B4_617 0.20 -1.6
713,996 + -2.7
714,126 - Pf1N1B4_617 0.41 -0.1
714,126 - -0.1
714,315 - Pf1N1B4_617 0.61 -0.1
714,315 - Pf1N1B4_617 0.61 -0.5

Or see this region's nucleotide sequence