Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5971 and Pf1N1B4_5972 overlap by 8 nucleotides Pf1N1B4_5972 and Pf1N1B4_5973 are separated by 10 nucleotides Pf1N1B4_5973 and Pf1N1B4_5974 overlap by 8 nucleotides Pf1N1B4_5974 and Pf1N1B4_5975 are separated by 134 nucleotides
Pf1N1B4_5971 - 2-Methylcitrate dehydratase AcnD, at 418,726 to 420,504
_5971
Pf1N1B4_5972 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 420,497 to 421,162
_5972
Pf1N1B4_5973 - Probable permease of ABC transporter, at 421,173 to 421,823
_5973
Pf1N1B4_5974 - ABC-type polar amino acid transport system, ATPase component, at 421,816 to 422,538
_5974
Pf1N1B4_5975 - 2-methylaconitate racemase, at 422,673 to 423,701
_5975
Position (kb) 421
422 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 420.192 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.507 kb on - strand at 420.507 kb on - strand at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.729 kb on - strand, within Pf1N1B4_5972 at 421.040 kb on + strand, within Pf1N1B4_5972 at 421.041 kb on - strand, within Pf1N1B4_5972 at 421.094 kb on + strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.098 kb on - strand at 421.458 kb on + strand, within Pf1N1B4_5973 at 421.470 kb on + strand, within Pf1N1B4_5973 at 421.748 kb on + strand, within Pf1N1B4_5973 at 421.748 kb on + strand, within Pf1N1B4_5973 at 421.749 kb on - strand, within Pf1N1B4_5973 at 421.753 kb on - strand, within Pf1N1B4_5973 at 421.755 kb on + strand, within Pf1N1B4_5973 at 421.755 kb on + strand, within Pf1N1B4_5973 at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.836 kb on - strand at 421.836 kb on - strand at 421.836 kb on - strand at 421.839 kb on + strand at 421.839 kb on + strand at 421.839 kb on + strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.840 kb on - strand at 422.284 kb on + strand at 422.285 kb on - strand, within Pf1N1B4_5974 at 422.613 kb on + strand at 422.613 kb on + strand at 422.613 kb on + strand at 422.614 kb on - strand at 422.614 kb on - strand at 422.698 kb on + strand at 422.698 kb on + strand at 422.698 kb on + strand at 422.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 420,192 + -0.0 420,352 + -1.5 420,352 + -1.7 420,352 + -0.0 420,353 - -2.0 420,353 - -2.9 420,353 - -0.0 420,353 - -0.0 420,507 - -0.0 420,507 - -0.0 420,720 - Pf1N1B4_5972 0.33 -0.8 420,720 - Pf1N1B4_5972 0.33 -0.0 420,729 - Pf1N1B4_5972 0.35 -0.0 421,040 + Pf1N1B4_5972 0.82 -0.0 421,041 - Pf1N1B4_5972 0.82 -0.0 421,094 + Pf1N1B4_5972 0.90 -4.2 421,095 - Pf1N1B4_5972 0.90 -0.0 421,095 - Pf1N1B4_5972 0.90 -0.0 421,098 - -1.7 421,458 + Pf1N1B4_5973 0.44 -0.0 421,470 + Pf1N1B4_5973 0.46 -2.8 421,748 + Pf1N1B4_5973 0.88 -2.2 421,748 + Pf1N1B4_5973 0.88 -0.0 421,749 - Pf1N1B4_5973 0.88 -2.1 421,753 - Pf1N1B4_5973 0.89 -4.2 421,755 + Pf1N1B4_5973 0.89 -2.0 421,755 + Pf1N1B4_5973 0.89 -0.0 421,835 + -1.7 421,835 + -3.8 421,835 + -0.0 421,835 + -2.0 421,835 + -1.0 421,835 + -0.0 421,836 - -0.0 421,836 - -0.0 421,836 - -0.0 421,839 + -0.0 421,839 + -1.0 421,839 + -3.0 421,840 - -0.0 421,840 - -1.6 421,840 - -0.0 421,840 - -1.5 422,284 + -0.0 422,285 - Pf1N1B4_5974 0.65 -0.0 422,613 + -4.3 422,613 + -0.0 422,613 + -1.7 422,614 - -0.0 422,614 - -2.1 422,698 + -1.9 422,698 + -0.0 422,698 + -1.1 422,699 - -0.0
Or see this region's nucleotide sequence