Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5841 and Pf1N1B4_5842 are separated by 137 nucleotides Pf1N1B4_5842 and Pf1N1B4_5843 are separated by 11 nucleotides Pf1N1B4_5843 and Pf1N1B4_5844 are separated by 164 nucleotides
Pf1N1B4_5841 - Radical SAM domain protein, at 275,158 to 276,960
_5841
Pf1N1B4_5842 - Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase), at 277,098 to 277,685
_5842
Pf1N1B4_5843 - phospholipase/carboxylesterase family protein, at 277,697 to 278,413
_5843
Pf1N1B4_5844 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 278,578 to 279,357
_5844
Position (kb) 277
278 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 276.106 kb on + strand, within Pf1N1B4_5841 at 276.107 kb on - strand, within Pf1N1B4_5841 at 276.238 kb on + strand at 276.463 kb on + strand, within Pf1N1B4_5841 at 276.463 kb on + strand, within Pf1N1B4_5841 at 276.464 kb on - strand, within Pf1N1B4_5841 at 276.464 kb on - strand, within Pf1N1B4_5841 at 276.464 kb on - strand, within Pf1N1B4_5841 at 276.472 kb on + strand, within Pf1N1B4_5841 at 276.681 kb on + strand, within Pf1N1B4_5841 at 276.682 kb on - strand, within Pf1N1B4_5841 at 276.717 kb on + strand, within Pf1N1B4_5841 at 276.718 kb on - strand, within Pf1N1B4_5841 at 276.776 kb on - strand, within Pf1N1B4_5841 at 276.776 kb on - strand, within Pf1N1B4_5841 at 276.776 kb on - strand, within Pf1N1B4_5841 at 276.787 kb on + strand at 276.787 kb on + strand at 276.787 kb on + strand at 276.787 kb on + strand at 276.788 kb on - strand at 276.788 kb on - strand at 276.788 kb on - strand at 276.788 kb on - strand at 276.788 kb on - strand at 276.802 kb on + strand at 277.112 kb on + strand at 277.683 kb on + strand at 277.683 kb on + strand at 277.684 kb on - strand at 278.034 kb on - strand, within Pf1N1B4_5843 at 278.056 kb on - strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.206 kb on - strand, within Pf1N1B4_5843 at 278.211 kb on + strand, within Pf1N1B4_5843 at 278.325 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.414 kb on - strand at 278.453 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.570 kb on - strand at 278.577 kb on - strand at 278.577 kb on - strand at 278.579 kb on + strand at 278.580 kb on - strand at 278.606 kb on + strand at 278.606 kb on + strand at 278.607 kb on - strand at 278.607 kb on - strand at 278.627 kb on + strand at 278.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 276,106 + Pf1N1B4_5841 0.53 -0.0 276,107 - Pf1N1B4_5841 0.53 -0.0 276,238 + -0.0 276,463 + Pf1N1B4_5841 0.72 -0.0 276,463 + Pf1N1B4_5841 0.72 -0.0 276,464 - Pf1N1B4_5841 0.72 -0.0 276,464 - Pf1N1B4_5841 0.72 -0.0 276,464 - Pf1N1B4_5841 0.72 -0.0 276,472 + Pf1N1B4_5841 0.73 -1.3 276,681 + Pf1N1B4_5841 0.84 -1.8 276,682 - Pf1N1B4_5841 0.85 -0.0 276,717 + Pf1N1B4_5841 0.86 -0.0 276,718 - Pf1N1B4_5841 0.87 -2.2 276,776 - Pf1N1B4_5841 0.90 -0.0 276,776 - Pf1N1B4_5841 0.90 -0.0 276,776 - Pf1N1B4_5841 0.90 -0.0 276,787 + -1.6 276,787 + -1.1 276,787 + -1.1 276,787 + -2.2 276,788 - -0.0 276,788 - -0.0 276,788 - -0.0 276,788 - -1.2 276,788 - -3.0 276,802 + -0.0 277,112 + -2.0 277,683 + -0.0 277,683 + -0.0 277,684 - -1.7 278,034 - Pf1N1B4_5843 0.47 -0.0 278,056 - Pf1N1B4_5843 0.50 -0.0 278,205 + Pf1N1B4_5843 0.71 +1.0 278,205 + Pf1N1B4_5843 0.71 -0.0 278,205 + Pf1N1B4_5843 0.71 -0.0 278,205 + Pf1N1B4_5843 0.71 -0.7 278,206 - Pf1N1B4_5843 0.71 -0.0 278,211 + Pf1N1B4_5843 0.72 -0.0 278,325 + -1.8 278,413 + -0.0 278,413 + -0.0 278,413 + -0.0 278,413 + -0.0 278,413 + -1.3 278,413 + -1.8 278,413 + -1.4 278,414 - -1.9 278,453 + -0.0 278,521 + -1.0 278,521 + -0.0 278,521 + -1.9 278,521 + -1.7 278,521 + -0.0 278,570 - -0.0 278,577 - -0.0 278,577 - -0.9 278,579 + -0.0 278,580 - -2.1 278,606 + -0.0 278,606 + -0.5 278,607 - -0.0 278,607 - -0.0 278,627 + -0.0 278,628 - -1.9
Or see this region's nucleotide sequence