Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_558

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_556 and Pf1N1B4_557 are separated by 83 nucleotidesPf1N1B4_557 and Pf1N1B4_558 are separated by 215 nucleotidesPf1N1B4_558 and Pf1N1B4_559 are separated by 67 nucleotidesPf1N1B4_559 and Pf1N1B4_560 are separated by 65 nucleotides Pf1N1B4_556 - Gluconate permease, at 653,037 to 654,389 _556 Pf1N1B4_557 - Gluconokinase (EC 2.7.1.12), at 654,473 to 655,006 _557 Pf1N1B4_558 - Gluconate utilization system Gnt-I transcriptional repressor, at 655,222 to 656,253 _558 Pf1N1B4_559 - Uncharacterized glutathione S-transferase-like protein, at 656,321 to 656,953 _559 Pf1N1B4_560 - Transcriptional regulators, LysR family, at 657,019 to 657,951 _560 Position (kb) 655 656 657Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 654.326 kb on + strandat 654.327 kb on - strandat 654.354 kb on + strandat 654.923 kb on - strand, within Pf1N1B4_557at 655.009 kb on - strandat 655.047 kb on - strandat 655.047 kb on - strandat 655.189 kb on + strandat 655.189 kb on + strandat 655.227 kb on + strandat 655.248 kb on + strandat 655.312 kb on - strandat 655.396 kb on + strand, within Pf1N1B4_558at 655.396 kb on + strand, within Pf1N1B4_558at 655.396 kb on + strandat 655.396 kb on + strand, within Pf1N1B4_558at 655.396 kb on + strandat 655.396 kb on + strand, within Pf1N1B4_558at 655.396 kb on + strand, within Pf1N1B4_558at 655.396 kb on + strand, within Pf1N1B4_558at 655.396 kb on + strand, within Pf1N1B4_558at 655.397 kb on - strand, within Pf1N1B4_558at 655.397 kb on - strand, within Pf1N1B4_558at 655.397 kb on - strand, within Pf1N1B4_558at 655.397 kb on - strand, within Pf1N1B4_558at 655.397 kb on - strand, within Pf1N1B4_558at 655.397 kb on - strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.594 kb on + strand, within Pf1N1B4_558at 655.595 kb on - strand, within Pf1N1B4_558at 655.595 kb on - strand, within Pf1N1B4_558at 655.595 kb on - strand, within Pf1N1B4_558at 655.604 kb on - strand, within Pf1N1B4_558at 655.690 kb on + strand, within Pf1N1B4_558at 655.690 kb on + strand, within Pf1N1B4_558at 655.691 kb on - strand, within Pf1N1B4_558at 655.691 kb on - strand, within Pf1N1B4_558at 655.691 kb on - strand, within Pf1N1B4_558at 655.804 kb on + strand, within Pf1N1B4_558at 655.804 kb on + strand, within Pf1N1B4_558at 655.804 kb on + strand, within Pf1N1B4_558at 655.804 kb on + strandat 655.804 kb on + strand, within Pf1N1B4_558at 655.804 kb on + strand, within Pf1N1B4_558at 655.804 kb on + strand, within Pf1N1B4_558at 655.805 kb on - strand, within Pf1N1B4_558at 655.805 kb on - strand, within Pf1N1B4_558at 655.882 kb on + strand, within Pf1N1B4_558at 655.882 kb on + strand, within Pf1N1B4_558at 656.282 kb on + strandat 656.283 kb on - strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.329 kb on + strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.330 kb on - strandat 656.530 kb on + strand, within Pf1N1B4_559at 656.530 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strandat 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.783 kb on + strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.784 kb on - strand, within Pf1N1B4_559at 656.923 kb on + strandat 657.123 kb on + strand, within Pf1N1B4_560at 657.124 kb on - strand, within Pf1N1B4_560at 657.124 kb on - strand, within Pf1N1B4_560at 657.124 kb on - strand, within Pf1N1B4_560at 657.124 kb on - strand, within Pf1N1B4_560at 657.124 kb on - strand, within Pf1N1B4_560at 657.124 kb on - strand, within Pf1N1B4_560at 657.187 kb on + strand, within Pf1N1B4_560at 657.187 kb on + strand, within Pf1N1B4_560at 657.188 kb on - strand, within Pf1N1B4_560

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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654,326 + -0.1
654,327 - -0.1
654,354 + -0.1
654,923 - Pf1N1B4_557 0.84 -0.1
655,009 - -0.1
655,047 - -1.8
655,047 - -2.0
655,189 + -1.7
655,189 + -4.5
655,227 + -0.1
655,248 + -0.1
655,312 - -1.7
655,396 + Pf1N1B4_558 0.17 -1.6
655,396 + Pf1N1B4_558 0.17 -2.3
655,396 + -0.1
655,396 + Pf1N1B4_558 0.17 -0.1
655,396 + -1.1
655,396 + Pf1N1B4_558 0.17 -0.1
655,396 + Pf1N1B4_558 0.17 -2.9
655,396 + Pf1N1B4_558 0.17 -0.1
655,396 + Pf1N1B4_558 0.17 -0.1
655,397 - Pf1N1B4_558 0.17 -0.1
655,397 - Pf1N1B4_558 0.17 -3.3
655,397 - Pf1N1B4_558 0.17 -0.1
655,397 - Pf1N1B4_558 0.17 -0.1
655,397 - Pf1N1B4_558 0.17 -2.3
655,397 - Pf1N1B4_558 0.17 -3.0
655,594 + Pf1N1B4_558 0.36 -3.0
655,594 + Pf1N1B4_558 0.36 -2.5
655,594 + Pf1N1B4_558 0.36 -0.1
655,594 + Pf1N1B4_558 0.36 -3.2
655,594 + Pf1N1B4_558 0.36 -4.1
655,594 + Pf1N1B4_558 0.36 -2.5
655,594 + Pf1N1B4_558 0.36 -2.9
655,594 + Pf1N1B4_558 0.36 -0.1
655,595 - Pf1N1B4_558 0.36 -0.1
655,595 - Pf1N1B4_558 0.36 -0.9
655,595 - Pf1N1B4_558 0.36 -0.1
655,604 - Pf1N1B4_558 0.37 -2.6
655,690 + Pf1N1B4_558 0.45 -0.1
655,690 + Pf1N1B4_558 0.45 -2.9
655,691 - Pf1N1B4_558 0.45 -0.1
655,691 - Pf1N1B4_558 0.45 -0.1
655,691 - Pf1N1B4_558 0.45 -3.2
655,804 + Pf1N1B4_558 0.56 -3.1
655,804 + Pf1N1B4_558 0.56 -0.1
655,804 + Pf1N1B4_558 0.56 -0.1
655,804 + -2.5
655,804 + Pf1N1B4_558 0.56 -2.8
655,804 + Pf1N1B4_558 0.56 -0.1
655,804 + Pf1N1B4_558 0.56 -0.1
655,805 - Pf1N1B4_558 0.56 -3.2
655,805 - Pf1N1B4_558 0.56 -2.8
655,882 + Pf1N1B4_558 0.64 -0.1
655,882 + Pf1N1B4_558 0.64 -2.9
656,282 + -1.0
656,283 - -0.1
656,329 + -1.1
656,329 + -0.1
656,329 + -0.1
656,329 + -1.2
656,329 + -2.0
656,329 + -1.5
656,329 + -1.8
656,329 + -0.1
656,329 + -0.1
656,330 - -0.1
656,330 - -0.1
656,330 - -2.3
656,330 - -0.1
656,330 - -0.1
656,330 - -1.9
656,330 - -0.1
656,330 - -0.1
656,330 - -1.7
656,330 - -0.1
656,530 + Pf1N1B4_559 0.33 -0.1
656,530 + Pf1N1B4_559 0.33 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -3.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -4.4
656,783 + Pf1N1B4_559 0.73 -2.0
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,783 + Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -1.8
656,784 - Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -0.8
656,784 - Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -1.8
656,784 - Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -2.1
656,784 - Pf1N1B4_559 0.73 -0.1
656,784 - Pf1N1B4_559 0.73 -1.2
656,784 - Pf1N1B4_559 0.73 -2.8
656,923 + -2.1
657,123 + Pf1N1B4_560 0.11 -2.6
657,124 - Pf1N1B4_560 0.11 -0.8
657,124 - Pf1N1B4_560 0.11 -1.8
657,124 - Pf1N1B4_560 0.11 -2.7
657,124 - Pf1N1B4_560 0.11 -0.1
657,124 - Pf1N1B4_560 0.11 -0.1
657,124 - Pf1N1B4_560 0.11 -0.1
657,187 + Pf1N1B4_560 0.18 -0.1
657,187 + Pf1N1B4_560 0.18 -2.4
657,188 - Pf1N1B4_560 0.18 -1.4

Or see this region's nucleotide sequence