Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_556 and Pf1N1B4_557 are separated by 83 nucleotides Pf1N1B4_557 and Pf1N1B4_558 are separated by 215 nucleotides Pf1N1B4_558 and Pf1N1B4_559 are separated by 67 nucleotides Pf1N1B4_559 and Pf1N1B4_560 are separated by 65 nucleotides
Pf1N1B4_556 - Gluconate permease, at 653,037 to 654,389
_556
Pf1N1B4_557 - Gluconokinase (EC 2.7.1.12), at 654,473 to 655,006
_557
Pf1N1B4_558 - Gluconate utilization system Gnt-I transcriptional repressor, at 655,222 to 656,253
_558
Pf1N1B4_559 - Uncharacterized glutathione S-transferase-like protein, at 656,321 to 656,953
_559
Pf1N1B4_560 - Transcriptional regulators, LysR family, at 657,019 to 657,951
_560
Position (kb)
655
656
657 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 654.326 kb on + strand at 654.327 kb on - strand at 654.354 kb on + strand at 654.923 kb on - strand, within Pf1N1B4_557 at 655.009 kb on - strand at 655.047 kb on - strand at 655.047 kb on - strand at 655.189 kb on + strand at 655.189 kb on + strand at 655.227 kb on + strand at 655.248 kb on + strand at 655.312 kb on - strand at 655.396 kb on + strand, within Pf1N1B4_558 at 655.396 kb on + strand, within Pf1N1B4_558 at 655.396 kb on + strand at 655.396 kb on + strand, within Pf1N1B4_558 at 655.396 kb on + strand at 655.396 kb on + strand, within Pf1N1B4_558 at 655.396 kb on + strand, within Pf1N1B4_558 at 655.396 kb on + strand, within Pf1N1B4_558 at 655.396 kb on + strand, within Pf1N1B4_558 at 655.397 kb on - strand, within Pf1N1B4_558 at 655.397 kb on - strand, within Pf1N1B4_558 at 655.397 kb on - strand, within Pf1N1B4_558 at 655.397 kb on - strand, within Pf1N1B4_558 at 655.397 kb on - strand, within Pf1N1B4_558 at 655.397 kb on - strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.594 kb on + strand, within Pf1N1B4_558 at 655.595 kb on - strand, within Pf1N1B4_558 at 655.595 kb on - strand, within Pf1N1B4_558 at 655.595 kb on - strand, within Pf1N1B4_558 at 655.604 kb on - strand, within Pf1N1B4_558 at 655.690 kb on + strand, within Pf1N1B4_558 at 655.690 kb on + strand, within Pf1N1B4_558 at 655.691 kb on - strand, within Pf1N1B4_558 at 655.691 kb on - strand, within Pf1N1B4_558 at 655.691 kb on - strand, within Pf1N1B4_558 at 655.804 kb on + strand, within Pf1N1B4_558 at 655.804 kb on + strand, within Pf1N1B4_558 at 655.804 kb on + strand, within Pf1N1B4_558 at 655.804 kb on + strand at 655.804 kb on + strand, within Pf1N1B4_558 at 655.804 kb on + strand, within Pf1N1B4_558 at 655.804 kb on + strand, within Pf1N1B4_558 at 655.805 kb on - strand, within Pf1N1B4_558 at 655.805 kb on - strand, within Pf1N1B4_558 at 655.882 kb on + strand, within Pf1N1B4_558 at 655.882 kb on + strand, within Pf1N1B4_558 at 656.282 kb on + strand at 656.283 kb on - strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.329 kb on + strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.330 kb on - strand at 656.530 kb on + strand, within Pf1N1B4_559 at 656.530 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.783 kb on + strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.784 kb on - strand, within Pf1N1B4_559 at 656.923 kb on + strand at 657.123 kb on + strand, within Pf1N1B4_560 at 657.124 kb on - strand, within Pf1N1B4_560 at 657.124 kb on - strand, within Pf1N1B4_560 at 657.124 kb on - strand, within Pf1N1B4_560 at 657.124 kb on - strand, within Pf1N1B4_560 at 657.124 kb on - strand, within Pf1N1B4_560 at 657.124 kb on - strand, within Pf1N1B4_560 at 657.187 kb on + strand, within Pf1N1B4_560 at 657.187 kb on + strand, within Pf1N1B4_560 at 657.188 kb on - strand, within Pf1N1B4_560
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 654,326 + -0.1 654,327 - -0.1 654,354 + -0.1 654,923 - Pf1N1B4_557 0.84 -0.1 655,009 - -0.1 655,047 - -1.8 655,047 - -2.0 655,189 + -1.7 655,189 + -4.5 655,227 + -0.1 655,248 + -0.1 655,312 - -1.7 655,396 + Pf1N1B4_558 0.17 -1.6 655,396 + Pf1N1B4_558 0.17 -2.3 655,396 + -0.1 655,396 + Pf1N1B4_558 0.17 -0.1 655,396 + -1.1 655,396 + Pf1N1B4_558 0.17 -0.1 655,396 + Pf1N1B4_558 0.17 -2.9 655,396 + Pf1N1B4_558 0.17 -0.1 655,396 + Pf1N1B4_558 0.17 -0.1 655,397 - Pf1N1B4_558 0.17 -0.1 655,397 - Pf1N1B4_558 0.17 -3.3 655,397 - Pf1N1B4_558 0.17 -0.1 655,397 - Pf1N1B4_558 0.17 -0.1 655,397 - Pf1N1B4_558 0.17 -2.3 655,397 - Pf1N1B4_558 0.17 -3.0 655,594 + Pf1N1B4_558 0.36 -3.0 655,594 + Pf1N1B4_558 0.36 -2.5 655,594 + Pf1N1B4_558 0.36 -0.1 655,594 + Pf1N1B4_558 0.36 -3.2 655,594 + Pf1N1B4_558 0.36 -4.1 655,594 + Pf1N1B4_558 0.36 -2.5 655,594 + Pf1N1B4_558 0.36 -2.9 655,594 + Pf1N1B4_558 0.36 -0.1 655,595 - Pf1N1B4_558 0.36 -0.1 655,595 - Pf1N1B4_558 0.36 -0.9 655,595 - Pf1N1B4_558 0.36 -0.1 655,604 - Pf1N1B4_558 0.37 -2.6 655,690 + Pf1N1B4_558 0.45 -0.1 655,690 + Pf1N1B4_558 0.45 -2.9 655,691 - Pf1N1B4_558 0.45 -0.1 655,691 - Pf1N1B4_558 0.45 -0.1 655,691 - Pf1N1B4_558 0.45 -3.2 655,804 + Pf1N1B4_558 0.56 -3.1 655,804 + Pf1N1B4_558 0.56 -0.1 655,804 + Pf1N1B4_558 0.56 -0.1 655,804 + -2.5 655,804 + Pf1N1B4_558 0.56 -2.8 655,804 + Pf1N1B4_558 0.56 -0.1 655,804 + Pf1N1B4_558 0.56 -0.1 655,805 - Pf1N1B4_558 0.56 -3.2 655,805 - Pf1N1B4_558 0.56 -2.8 655,882 + Pf1N1B4_558 0.64 -0.1 655,882 + Pf1N1B4_558 0.64 -2.9 656,282 + -1.0 656,283 - -0.1 656,329 + -1.1 656,329 + -0.1 656,329 + -0.1 656,329 + -1.2 656,329 + -2.0 656,329 + -1.5 656,329 + -1.8 656,329 + -0.1 656,329 + -0.1 656,330 - -0.1 656,330 - -0.1 656,330 - -2.3 656,330 - -0.1 656,330 - -0.1 656,330 - -1.9 656,330 - -0.1 656,330 - -0.1 656,330 - -1.7 656,330 - -0.1 656,530 + Pf1N1B4_559 0.33 -0.1 656,530 + Pf1N1B4_559 0.33 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -3.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -4.4 656,783 + Pf1N1B4_559 0.73 -2.0 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,783 + Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -1.8 656,784 - Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -0.8 656,784 - Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -1.8 656,784 - Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -2.1 656,784 - Pf1N1B4_559 0.73 -0.1 656,784 - Pf1N1B4_559 0.73 -1.2 656,784 - Pf1N1B4_559 0.73 -2.8 656,923 + -2.1 657,123 + Pf1N1B4_560 0.11 -2.6 657,124 - Pf1N1B4_560 0.11 -0.8 657,124 - Pf1N1B4_560 0.11 -1.8 657,124 - Pf1N1B4_560 0.11 -2.7 657,124 - Pf1N1B4_560 0.11 -0.1 657,124 - Pf1N1B4_560 0.11 -0.1 657,124 - Pf1N1B4_560 0.11 -0.1 657,187 + Pf1N1B4_560 0.18 -0.1 657,187 + Pf1N1B4_560 0.18 -2.4 657,188 - Pf1N1B4_560 0.18 -1.4
Or see this region's nucleotide sequence