Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5529 and Pf1N1B4_5530 are separated by 161 nucleotides Pf1N1B4_5530 and Pf1N1B4_5531 are separated by 79 nucleotides
Pf1N1B4_5529 - hypothetical protein, at 288,321 to 289,550
_5529
Pf1N1B4_5530 - hypothetical protein, at 289,712 to 290,338
_5530
Pf1N1B4_5531 - hypothetical protein, at 290,418 to 290,804
_5531
Position (kb)
289
290
291 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 288.764 kb on + strand, within Pf1N1B4_5529 at 288.765 kb on - strand, within Pf1N1B4_5529 at 288.765 kb on - strand, within Pf1N1B4_5529 at 288.839 kb on + strand, within Pf1N1B4_5529 at 288.839 kb on + strand, within Pf1N1B4_5529 at 288.840 kb on - strand, within Pf1N1B4_5529 at 288.840 kb on - strand, within Pf1N1B4_5529 at 288.840 kb on - strand, within Pf1N1B4_5529 at 288.871 kb on + strand, within Pf1N1B4_5529 at 289.052 kb on - strand, within Pf1N1B4_5529 at 289.180 kb on + strand, within Pf1N1B4_5529 at 289.180 kb on + strand, within Pf1N1B4_5529 at 289.181 kb on - strand, within Pf1N1B4_5529 at 289.181 kb on - strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.201 kb on + strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.202 kb on - strand, within Pf1N1B4_5529 at 289.366 kb on + strand, within Pf1N1B4_5529 at 289.366 kb on + strand, within Pf1N1B4_5529 at 289.366 kb on + strand, within Pf1N1B4_5529 at 289.367 kb on - strand, within Pf1N1B4_5529 at 289.495 kb on + strand at 289.495 kb on + strand at 289.495 kb on + strand at 289.495 kb on + strand at 289.495 kb on + strand at 289.496 kb on - strand at 289.496 kb on - strand at 289.496 kb on - strand at 289.496 kb on - strand at 289.511 kb on + strand at 289.544 kb on + strand at 289.545 kb on - strand at 289.594 kb on + strand at 289.594 kb on + strand at 289.731 kb on + strand at 289.732 kb on - strand at 289.810 kb on - strand, within Pf1N1B4_5530 at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.992 kb on + strand at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.992 kb on + strand, within Pf1N1B4_5530 at 289.993 kb on - strand, within Pf1N1B4_5530 at 289.993 kb on - strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.041 kb on + strand at 290.041 kb on + strand, within Pf1N1B4_5530 at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.042 kb on - strand at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.042 kb on - strand, within Pf1N1B4_5530 at 290.059 kb on + strand, within Pf1N1B4_5530 at 290.068 kb on - strand, within Pf1N1B4_5530 at 290.095 kb on + strand, within Pf1N1B4_5530 at 290.105 kb on + strand at 290.106 kb on - strand, within Pf1N1B4_5530 at 290.152 kb on + strand, within Pf1N1B4_5530 at 290.153 kb on - strand, within Pf1N1B4_5530 at 290.153 kb on - strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.232 kb on + strand, within Pf1N1B4_5530 at 290.233 kb on - strand, within Pf1N1B4_5530 at 290.255 kb on + strand, within Pf1N1B4_5530 at 290.275 kb on + strand, within Pf1N1B4_5530 at 290.275 kb on + strand, within Pf1N1B4_5530 at 290.275 kb on + strand, within Pf1N1B4_5530 at 290.276 kb on - strand at 290.276 kb on - strand at 290.276 kb on - strand at 290.276 kb on - strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.306 kb on + strand at 290.307 kb on - strand at 290.307 kb on - strand at 290.307 kb on - strand at 290.307 kb on - strand at 290.408 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.419 kb on + strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.420 kb on - strand at 290.493 kb on + strand at 290.493 kb on + strand, within Pf1N1B4_5531 at 290.493 kb on + strand, within Pf1N1B4_5531 at 290.493 kb on + strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.494 kb on - strand, within Pf1N1B4_5531 at 290.497 kb on + strand, within Pf1N1B4_5531 at 290.498 kb on - strand, within Pf1N1B4_5531 at 290.498 kb on - strand, within Pf1N1B4_5531 at 290.506 kb on + strand, within Pf1N1B4_5531 at 290.772 kb on + strand at 290.878 kb on + strand at 290.878 kb on + strand at 290.878 kb on + strand at 290.879 kb on - strand at 290.879 kb on - strand at 290.925 kb on + strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.031 kb on - strand at 291.037 kb on + strand at 291.037 kb on + strand at 291.037 kb on + strand at 291.055 kb on + strand at 291.056 kb on - strand at 291.092 kb on + strand at 291.092 kb on + strand at 291.092 kb on + strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.093 kb on - strand at 291.097 kb on - strand at 291.097 kb on - strand at 291.181 kb on + strand at 291.182 kb on - strand at 291.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 288,764 + Pf1N1B4_5529 0.36 -0.1 288,765 - Pf1N1B4_5529 0.36 -0.1 288,765 - Pf1N1B4_5529 0.36 -1.3 288,839 + Pf1N1B4_5529 0.42 -0.1 288,839 + Pf1N1B4_5529 0.42 -0.1 288,840 - Pf1N1B4_5529 0.42 -0.1 288,840 - Pf1N1B4_5529 0.42 -0.1 288,840 - Pf1N1B4_5529 0.42 -2.6 288,871 + Pf1N1B4_5529 0.45 -0.1 289,052 - Pf1N1B4_5529 0.59 -0.1 289,180 + Pf1N1B4_5529 0.70 -0.1 289,180 + Pf1N1B4_5529 0.70 -0.1 289,181 - Pf1N1B4_5529 0.70 -1.9 289,181 - Pf1N1B4_5529 0.70 -0.1 289,201 + Pf1N1B4_5529 0.72 -1.5 289,201 + Pf1N1B4_5529 0.72 -0.1 289,201 + Pf1N1B4_5529 0.72 -0.1 289,201 + Pf1N1B4_5529 0.72 -0.1 289,202 - Pf1N1B4_5529 0.72 -0.1 289,202 - Pf1N1B4_5529 0.72 -0.1 289,202 - Pf1N1B4_5529 0.72 -4.0 289,202 - Pf1N1B4_5529 0.72 -0.1 289,366 + Pf1N1B4_5529 0.85 -1.4 289,366 + Pf1N1B4_5529 0.85 -0.1 289,366 + Pf1N1B4_5529 0.85 -2.9 289,367 - Pf1N1B4_5529 0.85 -0.1 289,495 + -0.1 289,495 + -1.0 289,495 + -0.1 289,495 + -0.1 289,495 + -1.1 289,496 - -0.1 289,496 - -2.3 289,496 - -0.1 289,496 - -0.1 289,511 + -0.1 289,544 + -1.3 289,545 - -0.1 289,594 + -1.8 289,594 + -1.2 289,731 + -2.4 289,732 - -2.3 289,810 - Pf1N1B4_5530 0.16 -2.6 289,992 + Pf1N1B4_5530 0.45 -0.1 289,992 + Pf1N1B4_5530 0.45 -0.1 289,992 + -0.1 289,992 + Pf1N1B4_5530 0.45 -0.1 289,992 + Pf1N1B4_5530 0.45 -0.1 289,993 - Pf1N1B4_5530 0.45 -2.0 289,993 - Pf1N1B4_5530 0.45 -0.1 290,041 + Pf1N1B4_5530 0.52 -2.0 290,041 + Pf1N1B4_5530 0.52 -0.1 290,041 + Pf1N1B4_5530 0.52 -0.1 290,041 + Pf1N1B4_5530 0.52 -2.7 290,041 + Pf1N1B4_5530 0.52 -0.1 290,041 + Pf1N1B4_5530 0.52 -2.0 290,041 + -2.0 290,041 + Pf1N1B4_5530 0.52 -0.1 290,042 - Pf1N1B4_5530 0.53 -0.1 290,042 - Pf1N1B4_5530 0.53 -0.1 290,042 - -2.1 290,042 - Pf1N1B4_5530 0.53 -0.1 290,042 - Pf1N1B4_5530 0.53 -0.1 290,059 + Pf1N1B4_5530 0.55 -0.1 290,068 - Pf1N1B4_5530 0.57 -1.7 290,095 + Pf1N1B4_5530 0.61 -1.6 290,105 + -0.1 290,106 - Pf1N1B4_5530 0.63 -0.1 290,152 + Pf1N1B4_5530 0.70 -0.1 290,153 - Pf1N1B4_5530 0.70 -3.7 290,153 - Pf1N1B4_5530 0.70 -2.5 290,232 + Pf1N1B4_5530 0.83 -0.1 290,232 + Pf1N1B4_5530 0.83 -0.1 290,232 + Pf1N1B4_5530 0.83 -1.5 290,232 + Pf1N1B4_5530 0.83 -0.1 290,232 + Pf1N1B4_5530 0.83 -1.5 290,233 - Pf1N1B4_5530 0.83 -0.1 290,255 + Pf1N1B4_5530 0.87 -0.1 290,275 + Pf1N1B4_5530 0.90 -1.7 290,275 + Pf1N1B4_5530 0.90 -0.1 290,275 + Pf1N1B4_5530 0.90 -1.4 290,276 - -0.1 290,276 - -0.1 290,276 - -2.0 290,276 - -1.5 290,306 + -0.1 290,306 + -0.1 290,306 + -0.1 290,306 + -0.1 290,306 + -3.3 290,307 - -2.1 290,307 - -0.1 290,307 - -2.0 290,307 - -0.1 290,408 + -2.7 290,419 + -2.1 290,419 + -1.3 290,419 + -1.6 290,419 + -3.2 290,419 + -2.4 290,419 + -0.1 290,419 + -0.1 290,420 - -0.4 290,420 - -0.1 290,420 - -0.1 290,420 - -0.1 290,420 - -0.5 290,420 - -1.9 290,493 + -2.3 290,493 + Pf1N1B4_5531 0.19 -0.1 290,493 + Pf1N1B4_5531 0.19 -0.1 290,493 + Pf1N1B4_5531 0.19 -0.1 290,494 - Pf1N1B4_5531 0.20 -1.8 290,494 - Pf1N1B4_5531 0.20 -0.1 290,494 - Pf1N1B4_5531 0.20 -0.1 290,494 - Pf1N1B4_5531 0.20 -0.1 290,497 + Pf1N1B4_5531 0.20 -0.1 290,498 - Pf1N1B4_5531 0.21 -2.1 290,498 - Pf1N1B4_5531 0.21 -1.2 290,506 + Pf1N1B4_5531 0.23 -0.1 290,772 + -1.6 290,878 + -1.2 290,878 + -0.1 290,878 + -0.1 290,879 - -0.1 290,879 - -0.1 290,925 + -2.0 291,031 - -1.4 291,031 - -2.8 291,031 - -0.1 291,031 - -1.5 291,031 - -0.1 291,037 + -2.2 291,037 + -0.1 291,037 + -0.1 291,055 + -0.1 291,056 - -0.1 291,092 + -0.1 291,092 + +0.9 291,092 + -0.1 291,093 - -1.1 291,093 - -0.1 291,093 - -0.1 291,093 - -0.1 291,093 - -0.1 291,093 - -0.1 291,093 - -1.8 291,097 - -0.1 291,097 - -0.1 291,181 + -0.1 291,182 - -0.1 291,182 - -0.1
Or see this region's nucleotide sequence