Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5389

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5388 and Pf1N1B4_5389 are separated by 566 nucleotidesPf1N1B4_5389 and Pf1N1B4_5390 are separated by 455 nucleotidesPf1N1B4_5390 and Pf1N1B4_5391 are separated by 100 nucleotides Pf1N1B4_5388 - hypothetical protein, at 119,847 to 120,035 _5388 Pf1N1B4_5389 - hypothetical protein, at 120,602 to 121,195 _5389 Pf1N1B4_5390 - hypothetical protein, at 121,651 to 121,899 _5390 Pf1N1B4_5391 - hypothetical protein, at 122,000 to 123,037 _5391 Position (kb) 120 121 122Strain fitness (log2 ratio) -3 -2 -1 0 1at 119.760 kb on + strandat 119.761 kb on - strandat 119.811 kb on + strandat 120.164 kb on - strandat 120.203 kb on + strandat 120.203 kb on + strandat 120.319 kb on + strandat 120.319 kb on + strandat 120.319 kb on + strandat 120.319 kb on + strandat 120.319 kb on + strandat 120.319 kb on + strandat 120.320 kb on - strandat 120.320 kb on - strandat 120.320 kb on - strandat 120.320 kb on - strandat 120.407 kb on - strandat 120.407 kb on - strandat 120.483 kb on + strandat 120.501 kb on + strandat 120.502 kb on - strandat 120.502 kb on - strandat 120.627 kb on - strandat 120.642 kb on + strandat 120.642 kb on + strandat 120.643 kb on - strandat 120.643 kb on - strandat 120.643 kb on - strandat 120.643 kb on - strandat 120.643 kb on - strandat 120.643 kb on - strandat 120.643 kb on - strandat 120.667 kb on - strand, within Pf1N1B4_5389at 120.707 kb on + strand, within Pf1N1B4_5389at 120.823 kb on + strand, within Pf1N1B4_5389at 120.936 kb on + strand, within Pf1N1B4_5389at 120.936 kb on + strand, within Pf1N1B4_5389at 120.936 kb on + strand, within Pf1N1B4_5389at 121.000 kb on - strandat 121.042 kb on + strand, within Pf1N1B4_5389at 121.042 kb on + strand, within Pf1N1B4_5389at 121.042 kb on + strand, within Pf1N1B4_5389at 121.043 kb on - strandat 121.043 kb on - strand, within Pf1N1B4_5389at 121.050 kb on + strand, within Pf1N1B4_5389at 121.051 kb on - strand, within Pf1N1B4_5389at 121.051 kb on - strand, within Pf1N1B4_5389at 121.077 kb on + strand, within Pf1N1B4_5389at 121.077 kb on + strand, within Pf1N1B4_5389at 121.077 kb on + strand, within Pf1N1B4_5389at 121.078 kb on - strand, within Pf1N1B4_5389at 121.078 kb on - strand, within Pf1N1B4_5389at 121.078 kb on - strand, within Pf1N1B4_5389at 121.157 kb on - strandat 121.364 kb on + strandat 121.364 kb on + strandat 121.364 kb on + strandat 121.399 kb on - strandat 121.606 kb on + strandat 121.607 kb on - strandat 121.659 kb on + strandat 121.941 kb on + strandat 121.942 kb on - strandat 121.944 kb on + strandat 122.077 kb on - strandat 122.154 kb on + strand, within Pf1N1B4_5391at 122.155 kb on - strand, within Pf1N1B4_5391

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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119,760 + -0.1
119,761 - -0.1
119,811 + -0.1
120,164 - -0.1
120,203 + -2.9
120,203 + -0.1
120,319 + -0.1
120,319 + -2.2
120,319 + -0.1
120,319 + -2.9
120,319 + -0.1
120,319 + -3.4
120,320 - -0.1
120,320 - -0.1
120,320 - -0.1
120,320 - -0.1
120,407 - -1.9
120,407 - -1.7
120,483 + -1.6
120,501 + -0.1
120,502 - -0.1
120,502 - -3.1
120,627 - -2.0
120,642 + -0.1
120,642 + -1.1
120,643 - -0.1
120,643 - -2.3
120,643 - -2.4
120,643 - -0.1
120,643 - -3.2
120,643 - -2.6
120,643 - -0.1
120,667 - Pf1N1B4_5389 0.11 -0.1
120,707 + Pf1N1B4_5389 0.18 -0.1
120,823 + Pf1N1B4_5389 0.37 -2.3
120,936 + Pf1N1B4_5389 0.56 -0.1
120,936 + Pf1N1B4_5389 0.56 -0.1
120,936 + Pf1N1B4_5389 0.56 -0.1
121,000 - -1.2
121,042 + Pf1N1B4_5389 0.74 -0.1
121,042 + Pf1N1B4_5389 0.74 -0.1
121,042 + Pf1N1B4_5389 0.74 -0.1
121,043 - -0.1
121,043 - Pf1N1B4_5389 0.74 -0.1
121,050 + Pf1N1B4_5389 0.75 -3.1
121,051 - Pf1N1B4_5389 0.76 -0.1
121,051 - Pf1N1B4_5389 0.76 -0.1
121,077 + Pf1N1B4_5389 0.80 -0.1
121,077 + Pf1N1B4_5389 0.80 -3.2
121,077 + Pf1N1B4_5389 0.80 -3.2
121,078 - Pf1N1B4_5389 0.80 -0.1
121,078 - Pf1N1B4_5389 0.80 -2.2
121,078 - Pf1N1B4_5389 0.80 -0.1
121,157 - -0.1
121,364 + -0.1
121,364 + -1.7
121,364 + -0.1
121,399 - -0.1
121,606 + -0.1
121,607 - -0.1
121,659 + -2.6
121,941 + -0.1
121,942 - -2.1
121,944 + -0.1
122,077 - -2.5
122,154 + Pf1N1B4_5391 0.15 -0.1
122,155 - Pf1N1B4_5391 0.15 -1.9

Or see this region's nucleotide sequence