Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotides Pf1N1B4_5135 and Pf1N1B4_5136 are separated by 124 nucleotides Pf1N1B4_5136 and Pf1N1B4_5137 are separated by 27 nucleotides
Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045
_5134
Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685
_5135
Pf1N1B4_5136 - Carboxynorspermidine dehydrogenase, at 5,714,810 to 5,716,045
_5136
Pf1N1B4_5137 - Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-), at 5,716,073 to 5,717,170
_5137
Position (kb)
5714
5715
5716
5717 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5713.936 kb on - strand, within Pf1N1B4_5134 at 5714.038 kb on - strand at 5714.168 kb on - strand, within Pf1N1B4_5135 at 5714.222 kb on - strand, within Pf1N1B4_5135 at 5714.222 kb on - strand, within Pf1N1B4_5135 at 5714.436 kb on - strand, within Pf1N1B4_5135 at 5714.438 kb on + strand, within Pf1N1B4_5135 at 5714.439 kb on - strand, within Pf1N1B4_5135 at 5714.439 kb on - strand, within Pf1N1B4_5135 at 5714.533 kb on + strand, within Pf1N1B4_5135 at 5714.616 kb on + strand, within Pf1N1B4_5135 at 5714.684 kb on - strand at 5714.771 kb on - strand at 5714.985 kb on + strand, within Pf1N1B4_5136 at 5714.986 kb on - strand, within Pf1N1B4_5136 at 5714.986 kb on - strand, within Pf1N1B4_5136 at 5715.042 kb on + strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.043 kb on - strand, within Pf1N1B4_5136 at 5715.234 kb on - strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.487 kb on - strand, within Pf1N1B4_5136 at 5715.536 kb on - strand, within Pf1N1B4_5136 at 5715.546 kb on + strand, within Pf1N1B4_5136 at 5715.657 kb on + strand at 5715.866 kb on + strand, within Pf1N1B4_5136 at 5715.867 kb on - strand, within Pf1N1B4_5136 at 5715.867 kb on - strand, within Pf1N1B4_5136 at 5715.867 kb on - strand, within Pf1N1B4_5136 at 5715.956 kb on - strand at 5716.028 kb on - strand at 5716.074 kb on + strand at 5716.093 kb on - strand at 5716.093 kb on - strand at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.309 kb on - strand, within Pf1N1B4_5137 at 5716.419 kb on + strand, within Pf1N1B4_5137 at 5716.851 kb on + strand at 5716.942 kb on - strand, within Pf1N1B4_5137 at 5717.005 kb on + strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.020 kb on - strand at 5717.020 kb on - strand at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.020 kb on - strand, within Pf1N1B4_5137
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 5,713,936 - Pf1N1B4_5134 0.87 +0.0 5,714,038 - +0.0 5,714,168 - Pf1N1B4_5135 0.20 +0.0 5,714,222 - Pf1N1B4_5135 0.28 -1.5 5,714,222 - Pf1N1B4_5135 0.28 -0.9 5,714,436 - Pf1N1B4_5135 0.61 -2.1 5,714,438 + Pf1N1B4_5135 0.62 -2.2 5,714,439 - Pf1N1B4_5135 0.62 -2.3 5,714,439 - Pf1N1B4_5135 0.62 -1.0 5,714,533 + Pf1N1B4_5135 0.76 +0.0 5,714,616 + Pf1N1B4_5135 0.89 +0.0 5,714,684 - -2.2 5,714,771 - +0.0 5,714,985 + Pf1N1B4_5136 0.14 +0.0 5,714,986 - Pf1N1B4_5136 0.14 +0.0 5,714,986 - Pf1N1B4_5136 0.14 +0.0 5,715,042 + Pf1N1B4_5136 0.19 +0.0 5,715,043 - Pf1N1B4_5136 0.19 +0.0 5,715,043 - Pf1N1B4_5136 0.19 -3.7 5,715,043 - Pf1N1B4_5136 0.19 -5.1 5,715,043 - Pf1N1B4_5136 0.19 -2.9 5,715,043 - Pf1N1B4_5136 0.19 +0.0 5,715,234 - Pf1N1B4_5136 0.34 +0.0 5,715,252 + Pf1N1B4_5136 0.36 -3.6 5,715,252 + Pf1N1B4_5136 0.36 +1.0 5,715,252 + Pf1N1B4_5136 0.36 +0.0 5,715,253 - Pf1N1B4_5136 0.36 -3.5 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,253 - -4.1 5,715,253 - Pf1N1B4_5136 0.36 -2.2 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,340 - Pf1N1B4_5136 0.43 +0.0 5,715,340 - Pf1N1B4_5136 0.43 +0.0 5,715,487 - Pf1N1B4_5136 0.55 -1.0 5,715,536 - Pf1N1B4_5136 0.59 -1.3 5,715,546 + Pf1N1B4_5136 0.60 +0.0 5,715,657 + +0.0 5,715,866 + Pf1N1B4_5136 0.85 -3.6 5,715,867 - Pf1N1B4_5136 0.86 +0.0 5,715,867 - Pf1N1B4_5136 0.86 -2.8 5,715,867 - Pf1N1B4_5136 0.86 -3.7 5,715,956 - +0.0 5,716,028 - -2.4 5,716,074 + -2.9 5,716,093 - +0.0 5,716,093 - -2.6 5,716,308 + Pf1N1B4_5137 0.21 -3.0 5,716,308 + -2.7 5,716,308 + Pf1N1B4_5137 0.21 -2.1 5,716,308 + +0.0 5,716,308 + Pf1N1B4_5137 0.21 +0.0 5,716,308 + Pf1N1B4_5137 0.21 +0.0 5,716,308 + Pf1N1B4_5137 0.21 +0.0 5,716,309 - Pf1N1B4_5137 0.21 -2.4 5,716,419 + Pf1N1B4_5137 0.32 -4.2 5,716,851 + +0.0 5,716,942 - Pf1N1B4_5137 0.79 +0.0 5,717,005 + Pf1N1B4_5137 0.85 -2.5 5,717,006 - Pf1N1B4_5137 0.85 +0.0 5,717,006 - Pf1N1B4_5137 0.85 -3.6 5,717,006 - Pf1N1B4_5137 0.85 -3.6 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -0.9 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -3.5 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -2.3 5,717,019 + Pf1N1B4_5137 0.86 -1.8 5,717,020 - -2.2 5,717,020 - -2.3 5,717,020 - Pf1N1B4_5137 0.86 -3.4 5,717,020 - Pf1N1B4_5137 0.86 -2.3 5,717,020 - Pf1N1B4_5137 0.86 +0.0
Or see this region's nucleotide sequence