Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4616 and Pf1N1B4_4617 are separated by 79 nucleotides Pf1N1B4_4617 and Pf1N1B4_4618 are separated by 55 nucleotides Pf1N1B4_4618 and Pf1N1B4_4619 are separated by 13 nucleotides
Pf1N1B4_4616 - FIG005548: transport protein, at 5,087,901 to 5,090,285
_4616
Pf1N1B4_4617 - hypothetical protein, at 5,090,365 to 5,092,734
_4617
Pf1N1B4_4618 - hypothetical protein, at 5,092,790 to 5,093,161
_4618
Pf1N1B4_4619 - hypothetical protein, at 5,093,175 to 5,095,172
_4619
Position (kb) 5090
5091
5092
5093 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5089.584 kb on + strand, within Pf1N1B4_4616 at 5089.584 kb on + strand at 5089.584 kb on + strand, within Pf1N1B4_4616 at 5089.798 kb on - strand, within Pf1N1B4_4616 at 5089.873 kb on + strand, within Pf1N1B4_4616 at 5089.987 kb on - strand, within Pf1N1B4_4616 at 5089.992 kb on + strand, within Pf1N1B4_4616 at 5089.992 kb on + strand, within Pf1N1B4_4616 at 5089.993 kb on - strand, within Pf1N1B4_4616 at 5089.993 kb on - strand, within Pf1N1B4_4616 at 5089.993 kb on - strand, within Pf1N1B4_4616 at 5089.993 kb on - strand, within Pf1N1B4_4616 at 5090.043 kb on + strand at 5090.044 kb on - strand, within Pf1N1B4_4616 at 5090.110 kb on + strand at 5090.110 kb on + strand at 5090.110 kb on + strand at 5090.501 kb on - strand at 5090.562 kb on - strand at 5090.612 kb on + strand, within Pf1N1B4_4617 at 5090.612 kb on + strand at 5090.612 kb on + strand, within Pf1N1B4_4617 at 5090.633 kb on - strand, within Pf1N1B4_4617 at 5090.678 kb on - strand, within Pf1N1B4_4617 at 5090.678 kb on - strand, within Pf1N1B4_4617 at 5090.678 kb on - strand, within Pf1N1B4_4617 at 5090.920 kb on + strand, within Pf1N1B4_4617 at 5090.995 kb on + strand, within Pf1N1B4_4617 at 5090.995 kb on + strand, within Pf1N1B4_4617 at 5091.092 kb on + strand, within Pf1N1B4_4617 at 5091.092 kb on + strand, within Pf1N1B4_4617 at 5091.141 kb on + strand, within Pf1N1B4_4617 at 5091.247 kb on - strand at 5091.247 kb on - strand, within Pf1N1B4_4617 at 5091.354 kb on - strand, within Pf1N1B4_4617 at 5091.668 kb on - strand, within Pf1N1B4_4617 at 5091.892 kb on + strand, within Pf1N1B4_4617 at 5091.892 kb on + strand, within Pf1N1B4_4617 at 5091.893 kb on - strand at 5091.932 kb on - strand, within Pf1N1B4_4617 at 5092.073 kb on - strand, within Pf1N1B4_4617 at 5092.169 kb on - strand, within Pf1N1B4_4617 at 5092.872 kb on - strand, within Pf1N1B4_4618 at 5093.011 kb on + strand, within Pf1N1B4_4618 at 5093.011 kb on + strand, within Pf1N1B4_4618 at 5093.124 kb on + strand at 5093.125 kb on - strand at 5093.310 kb on - strand at 5093.310 kb on - strand at 5093.395 kb on + strand, within Pf1N1B4_4619 at 5093.396 kb on - strand, within Pf1N1B4_4619 at 5093.456 kb on + strand, within Pf1N1B4_4619 at 5093.456 kb on + strand, within Pf1N1B4_4619 at 5093.456 kb on + strand, within Pf1N1B4_4619 at 5093.457 kb on - strand, within Pf1N1B4_4619 at 5093.620 kb on + strand, within Pf1N1B4_4619 at 5093.621 kb on - strand, within Pf1N1B4_4619
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 5,089,584 + Pf1N1B4_4616 0.71 +0.0 5,089,584 + -1.8 5,089,584 + Pf1N1B4_4616 0.71 -1.2 5,089,798 - Pf1N1B4_4616 0.80 +0.0 5,089,873 + Pf1N1B4_4616 0.83 -2.0 5,089,987 - Pf1N1B4_4616 0.87 -1.8 5,089,992 + Pf1N1B4_4616 0.88 +0.0 5,089,992 + Pf1N1B4_4616 0.88 -2.4 5,089,993 - Pf1N1B4_4616 0.88 +0.0 5,089,993 - Pf1N1B4_4616 0.88 -3.0 5,089,993 - Pf1N1B4_4616 0.88 +0.0 5,089,993 - Pf1N1B4_4616 0.88 +0.0 5,090,043 + -1.8 5,090,044 - Pf1N1B4_4616 0.90 +0.0 5,090,110 + -2.3 5,090,110 + +0.0 5,090,110 + +0.0 5,090,501 - +0.0 5,090,562 - +0.0 5,090,612 + Pf1N1B4_4617 0.10 -3.0 5,090,612 + +0.0 5,090,612 + Pf1N1B4_4617 0.10 -2.7 5,090,633 - Pf1N1B4_4617 0.11 -3.6 5,090,678 - Pf1N1B4_4617 0.13 -2.9 5,090,678 - Pf1N1B4_4617 0.13 -2.9 5,090,678 - Pf1N1B4_4617 0.13 -4.9 5,090,920 + Pf1N1B4_4617 0.23 -1.2 5,090,995 + Pf1N1B4_4617 0.27 +0.0 5,090,995 + Pf1N1B4_4617 0.27 +0.0 5,091,092 + Pf1N1B4_4617 0.31 +0.0 5,091,092 + Pf1N1B4_4617 0.31 +0.0 5,091,141 + Pf1N1B4_4617 0.33 +0.0 5,091,247 - -2.2 5,091,247 - Pf1N1B4_4617 0.37 +0.0 5,091,354 - Pf1N1B4_4617 0.42 +0.0 5,091,668 - Pf1N1B4_4617 0.55 +0.0 5,091,892 + Pf1N1B4_4617 0.64 +0.0 5,091,892 + Pf1N1B4_4617 0.64 +0.0 5,091,893 - +0.0 5,091,932 - Pf1N1B4_4617 0.66 +0.0 5,092,073 - Pf1N1B4_4617 0.72 +0.0 5,092,169 - Pf1N1B4_4617 0.76 +0.0 5,092,872 - Pf1N1B4_4618 0.22 +1.0 5,093,011 + Pf1N1B4_4618 0.59 -2.6 5,093,011 + Pf1N1B4_4618 0.59 -1.6 5,093,124 + +0.0 5,093,125 - -1.5 5,093,310 - +0.0 5,093,310 - -2.3 5,093,395 + Pf1N1B4_4619 0.11 -1.7 5,093,396 - Pf1N1B4_4619 0.11 -1.6 5,093,456 + Pf1N1B4_4619 0.14 +0.0 5,093,456 + Pf1N1B4_4619 0.14 +0.0 5,093,456 + Pf1N1B4_4619 0.14 +0.0 5,093,457 - Pf1N1B4_4619 0.14 -2.2 5,093,620 + Pf1N1B4_4619 0.22 +0.0 5,093,621 - Pf1N1B4_4619 0.22 -2.3
Or see this region's nucleotide sequence