Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4221 and Pf1N1B4_4222 are separated by 19 nucleotides Pf1N1B4_4222 and Pf1N1B4_4223 are separated by 6 nucleotides
Pf1N1B4_4221 - hypothetical protein, at 4,678,383 to 4,678,499
_4221
Pf1N1B4_4222 - putative tyrosinase, at 4,678,519 to 4,679,379
_4222
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Position (kb)
4678
4679
4680 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4677.553 kb on - strand at 4677.604 kb on + strand at 4677.671 kb on - strand at 4677.831 kb on + strand at 4677.831 kb on + strand at 4677.835 kb on + strand at 4677.836 kb on - strand at 4677.978 kb on + strand at 4677.979 kb on - strand at 4678.073 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.109 kb on - strand at 4678.126 kb on + strand at 4678.269 kb on + strand at 4678.270 kb on - strand at 4678.362 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.498 kb on - strand at 4678.531 kb on + strand at 4678.604 kb on - strand at 4678.604 kb on - strand at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4679.011 kb on + strand, within Pf1N1B4_4222 at 4679.012 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.098 kb on - strand, within Pf1N1B4_4222 at 4679.230 kb on + strand, within Pf1N1B4_4222 at 4679.231 kb on - strand at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.335 kb on + strand at 4679.336 kb on - strand at 4679.345 kb on - strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.353 kb on + strand at 4679.377 kb on + strand at 4679.377 kb on + strand at 4679.501 kb on + strand, within Pf1N1B4_4223 at 4679.502 kb on - strand at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.807 kb on + strand, within Pf1N1B4_4223 at 4679.884 kb on - strand at 4679.933 kb on + strand at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4680.137 kb on - strand, within Pf1N1B4_4223 at 4680.244 kb on - strand at 4680.303 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 4,677,553 - -0.0 4,677,604 + -2.6 4,677,671 - -0.8 4,677,831 + -0.0 4,677,831 + -1.4 4,677,835 + -0.0 4,677,836 - -2.5 4,677,978 + -0.0 4,677,979 - -0.0 4,678,073 + -2.0 4,678,108 + -0.0 4,678,108 + -0.0 4,678,108 + -0.0 4,678,108 + -0.0 4,678,108 + -1.0 4,678,108 + -0.0 4,678,109 - -3.2 4,678,126 + -0.0 4,678,269 + -0.0 4,678,270 - -2.6 4,678,362 + -0.0 4,678,497 + -0.0 4,678,497 + -0.0 4,678,497 + +0.6 4,678,497 + -0.0 4,678,498 - -0.0 4,678,531 + -3.1 4,678,604 - -0.0 4,678,604 - -0.8 4,678,846 + Pf1N1B4_4222 0.38 -0.0 4,678,846 + Pf1N1B4_4222 0.38 -0.0 4,679,011 + Pf1N1B4_4222 0.57 -0.0 4,679,012 - Pf1N1B4_4222 0.57 -0.0 4,679,027 - Pf1N1B4_4222 0.59 -1.8 4,679,027 - Pf1N1B4_4222 0.59 -0.8 4,679,027 - Pf1N1B4_4222 0.59 -0.0 4,679,098 - Pf1N1B4_4222 0.67 -0.0 4,679,230 + Pf1N1B4_4222 0.83 -1.6 4,679,231 - -2.0 4,679,234 + Pf1N1B4_4222 0.83 -1.2 4,679,234 + Pf1N1B4_4222 0.83 -0.0 4,679,235 - Pf1N1B4_4222 0.83 -0.0 4,679,235 - Pf1N1B4_4222 0.83 -1.2 4,679,290 + Pf1N1B4_4222 0.90 -0.0 4,679,290 + Pf1N1B4_4222 0.90 -0.0 4,679,291 - Pf1N1B4_4222 0.90 -0.0 4,679,291 - Pf1N1B4_4222 0.90 -1.7 4,679,291 - Pf1N1B4_4222 0.90 +0.1 4,679,335 + -0.0 4,679,336 - -0.0 4,679,345 - -2.2 4,679,347 + -2.1 4,679,347 + -0.0 4,679,347 + -0.0 4,679,347 + -0.0 4,679,348 - -1.7 4,679,348 - -3.9 4,679,348 - -0.0 4,679,353 + -1.3 4,679,377 + -0.0 4,679,377 + -0.0 4,679,501 + Pf1N1B4_4223 0.13 -0.0 4,679,502 - -2.7 4,679,734 + Pf1N1B4_4223 0.39 -1.0 4,679,734 + Pf1N1B4_4223 0.39 -0.9 4,679,807 + Pf1N1B4_4223 0.47 -2.8 4,679,884 - -0.0 4,679,933 + -2.6 4,679,934 - Pf1N1B4_4223 0.61 -0.0 4,679,934 - Pf1N1B4_4223 0.61 -2.5 4,680,137 - Pf1N1B4_4223 0.84 -0.0 4,680,244 - -3.5 4,680,303 + -0.0
Or see this region's nucleotide sequence