Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4101 and Pf1N1B4_4102 are separated by 96 nucleotides Pf1N1B4_4102 and Pf1N1B4_4103 are separated by 284 nucleotides Pf1N1B4_4103 and Pf1N1B4_4104 are separated by 201 nucleotides Pf1N1B4_4104 and Pf1N1B4_4105 are separated by 47 nucleotides
Pf1N1B4_4101 - Transcriptional regulator, AraC family, at 4,549,784 to 4,550,575
_4101
Pf1N1B4_4102 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 4,550,672 to 4,551,058
_4102
Pf1N1B4_4103 - Flagellar synthesis regulator FleN, at 4,551,343 to 4,552,164
_4103
Pf1N1B4_4104 - Probable transmembrane protein, at 4,552,366 to 4,552,851
_4104
Pf1N1B4_4105 - hypothetical protein, at 4,552,899 to 4,553,366
_4105
Position (kb)
4551
4552
4553 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4550.381 kb on + strand, within Pf1N1B4_4101 at 4550.382 kb on - strand, within Pf1N1B4_4101 at 4550.387 kb on - strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.402 kb on + strand, within Pf1N1B4_4101 at 4550.403 kb on - strand, within Pf1N1B4_4101 at 4550.403 kb on - strand, within Pf1N1B4_4101 at 4550.590 kb on + strand at 4550.630 kb on + strand at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.921 kb on + strand, within Pf1N1B4_4102 at 4550.922 kb on - strand, within Pf1N1B4_4102 at 4550.922 kb on - strand, within Pf1N1B4_4102 at 4550.979 kb on + strand, within Pf1N1B4_4102 at 4551.220 kb on + strand at 4551.461 kb on + strand, within Pf1N1B4_4103 at 4551.462 kb on - strand, within Pf1N1B4_4103 at 4551.462 kb on - strand, within Pf1N1B4_4103 at 4551.499 kb on - strand, within Pf1N1B4_4103 at 4551.653 kb on - strand at 4551.675 kb on + strand, within Pf1N1B4_4103 at 4552.179 kb on + strand at 4552.179 kb on + strand at 4552.179 kb on + strand at 4552.180 kb on - strand at 4552.279 kb on - strand at 4552.567 kb on + strand, within Pf1N1B4_4104 at 4552.567 kb on + strand, within Pf1N1B4_4104 at 4552.568 kb on - strand, within Pf1N1B4_4104 at 4552.568 kb on - strand, within Pf1N1B4_4104 at 4552.765 kb on - strand, within Pf1N1B4_4104
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 4,550,381 + Pf1N1B4_4101 0.75 -0.0 4,550,382 - Pf1N1B4_4101 0.76 -2.9 4,550,387 - Pf1N1B4_4101 0.76 -1.4 4,550,402 + Pf1N1B4_4101 0.78 -0.0 4,550,402 + Pf1N1B4_4101 0.78 -2.0 4,550,402 + Pf1N1B4_4101 0.78 -2.0 4,550,403 - Pf1N1B4_4101 0.78 -1.6 4,550,403 - Pf1N1B4_4101 0.78 -0.0 4,550,590 + -0.0 4,550,630 + -0.0 4,550,921 + Pf1N1B4_4102 0.64 -3.0 4,550,921 + Pf1N1B4_4102 0.64 -0.0 4,550,921 + Pf1N1B4_4102 0.64 -1.1 4,550,922 - Pf1N1B4_4102 0.65 -0.0 4,550,922 - Pf1N1B4_4102 0.65 -0.0 4,550,979 + Pf1N1B4_4102 0.79 -0.0 4,551,220 + -1.0 4,551,461 + Pf1N1B4_4103 0.14 -0.0 4,551,462 - Pf1N1B4_4103 0.14 -0.0 4,551,462 - Pf1N1B4_4103 0.14 -2.6 4,551,499 - Pf1N1B4_4103 0.19 -3.7 4,551,653 - -2.4 4,551,675 + Pf1N1B4_4103 0.40 -0.0 4,552,179 + -0.0 4,552,179 + -0.0 4,552,179 + -0.0 4,552,180 - -0.0 4,552,279 - -0.0 4,552,567 + Pf1N1B4_4104 0.41 -2.5 4,552,567 + Pf1N1B4_4104 0.41 -2.1 4,552,568 - Pf1N1B4_4104 0.42 -2.0 4,552,568 - Pf1N1B4_4104 0.42 -2.3 4,552,765 - Pf1N1B4_4104 0.82 -0.0
Or see this region's nucleotide sequence