Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3597 and Pf1N1B4_3598 are separated by 10 nucleotides Pf1N1B4_3598 and Pf1N1B4_3599 are separated by 172 nucleotides Pf1N1B4_3599 and Pf1N1B4_3600 are separated by 110 nucleotides
Pf1N1B4_3597 - Flagellar biosynthesis protein FliS, at 4,008,857 to 4,009,252
_3597
Pf1N1B4_3598 - FIG00953167: hypothetical protein, at 4,009,263 to 4,009,559
_3598
Pf1N1B4_3599 - Flagellar regulatory protein FleQ, at 4,009,732 to 4,011,207
_3599
Pf1N1B4_3600 - Flagellar sensor histidine kinase FleS, at 4,011,318 to 4,012,541
_3600
Position (kb)
4009
4010
4011
4012 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4008.791 kb on - strand at 4008.824 kb on - strand at 4008.825 kb on + strand at 4008.826 kb on - strand at 4008.826 kb on - strand at 4008.826 kb on - strand at 4008.832 kb on + strand at 4008.832 kb on + strand at 4008.833 kb on - strand at 4008.833 kb on - strand at 4008.833 kb on - strand at 4008.851 kb on + strand at 4008.851 kb on + strand at 4008.852 kb on - strand at 4008.852 kb on - strand at 4008.852 kb on - strand at 4008.852 kb on - strand at 4008.870 kb on + strand at 4009.112 kb on - strand, within Pf1N1B4_3597 at 4009.118 kb on + strand, within Pf1N1B4_3597 at 4009.119 kb on - strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.124 kb on + strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.125 kb on - strand, within Pf1N1B4_3597 at 4009.251 kb on - strand at 4009.251 kb on - strand at 4009.273 kb on + strand at 4009.273 kb on + strand at 4009.274 kb on - strand at 4009.274 kb on - strand at 4009.274 kb on - strand at 4009.449 kb on + strand, within Pf1N1B4_3598 at 4009.449 kb on + strand, within Pf1N1B4_3598 at 4009.450 kb on - strand, within Pf1N1B4_3598 at 4009.450 kb on - strand, within Pf1N1B4_3598 at 4009.450 kb on - strand at 4009.451 kb on + strand, within Pf1N1B4_3598 at 4009.451 kb on + strand, within Pf1N1B4_3598 at 4009.451 kb on + strand, within Pf1N1B4_3598 at 4009.451 kb on + strand, within Pf1N1B4_3598 at 4009.452 kb on - strand, within Pf1N1B4_3598 at 4009.561 kb on + strand at 4009.583 kb on + strand at 4009.583 kb on + strand at 4009.583 kb on + strand at 4009.583 kb on + strand at 4009.622 kb on + strand at 4009.622 kb on + strand at 4009.681 kb on + strand at 4009.886 kb on + strand, within Pf1N1B4_3599 at 4009.886 kb on + strand, within Pf1N1B4_3599 at 4009.887 kb on - strand, within Pf1N1B4_3599 at 4009.887 kb on - strand, within Pf1N1B4_3599 at 4009.887 kb on - strand, within Pf1N1B4_3599 at 4009.887 kb on - strand, within Pf1N1B4_3599 at 4009.941 kb on - strand, within Pf1N1B4_3599 at 4010.062 kb on + strand, within Pf1N1B4_3599 at 4010.113 kb on + strand, within Pf1N1B4_3599 at 4010.113 kb on + strand, within Pf1N1B4_3599 at 4010.113 kb on + strand, within Pf1N1B4_3599 at 4010.113 kb on + strand, within Pf1N1B4_3599 at 4010.346 kb on + strand, within Pf1N1B4_3599 at 4010.451 kb on + strand, within Pf1N1B4_3599 at 4010.641 kb on + strand, within Pf1N1B4_3599 at 4010.641 kb on + strand, within Pf1N1B4_3599 at 4010.642 kb on - strand, within Pf1N1B4_3599 at 4011.163 kb on + strand at 4011.164 kb on - strand at 4011.232 kb on + strand at 4011.233 kb on - strand at 4011.234 kb on + strand at 4011.234 kb on + strand at 4011.234 kb on + strand at 4011.264 kb on + strand at 4011.264 kb on + strand at 4011.264 kb on + strand at 4011.453 kb on + strand, within Pf1N1B4_3600 at 4011.629 kb on + strand, within Pf1N1B4_3600 at 4011.758 kb on - strand, within Pf1N1B4_3600 at 4011.882 kb on - strand, within Pf1N1B4_3600 at 4011.936 kb on + strand, within Pf1N1B4_3600 at 4011.937 kb on - strand, within Pf1N1B4_3600 at 4012.191 kb on - strand, within Pf1N1B4_3600
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 4,008,791 - +0.1 4,008,824 - +0.1 4,008,825 + +0.1 4,008,826 - +0.1 4,008,826 - +0.1 4,008,826 - -2.4 4,008,832 + -2.4 4,008,832 + +0.1 4,008,833 - +0.1 4,008,833 - +0.1 4,008,833 - +0.1 4,008,851 + +0.1 4,008,851 + +0.1 4,008,852 - -3.9 4,008,852 - +0.1 4,008,852 - -3.1 4,008,852 - +0.1 4,008,870 + +0.1 4,009,112 - Pf1N1B4_3597 0.64 +0.1 4,009,118 + Pf1N1B4_3597 0.66 +0.1 4,009,119 - Pf1N1B4_3597 0.66 +0.1 4,009,124 + Pf1N1B4_3597 0.67 +0.1 4,009,124 + Pf1N1B4_3597 0.67 -3.1 4,009,124 + Pf1N1B4_3597 0.67 +0.1 4,009,124 + Pf1N1B4_3597 0.67 +0.1 4,009,124 + Pf1N1B4_3597 0.67 -2.8 4,009,124 + Pf1N1B4_3597 0.67 +0.1 4,009,124 + +0.1 4,009,124 + Pf1N1B4_3597 0.67 +0.1 4,009,124 + Pf1N1B4_3597 0.67 -1.6 4,009,124 + Pf1N1B4_3597 0.67 -2.7 4,009,124 + Pf1N1B4_3597 0.67 +0.1 4,009,125 - Pf1N1B4_3597 0.68 -2.7 4,009,125 - Pf1N1B4_3597 0.68 -3.4 4,009,125 - Pf1N1B4_3597 0.68 -2.0 4,009,125 - Pf1N1B4_3597 0.68 +0.1 4,009,125 - Pf1N1B4_3597 0.68 -2.2 4,009,125 - Pf1N1B4_3597 0.68 -0.9 4,009,125 - Pf1N1B4_3597 0.68 -2.5 4,009,125 - Pf1N1B4_3597 0.68 +0.1 4,009,125 - Pf1N1B4_3597 0.68 -1.6 4,009,125 - Pf1N1B4_3597 0.68 -2.2 4,009,125 - Pf1N1B4_3597 0.68 +0.1 4,009,125 - Pf1N1B4_3597 0.68 +0.1 4,009,251 - +0.0 4,009,251 - -2.7 4,009,273 + -3.1 4,009,273 + -2.8 4,009,274 - -1.4 4,009,274 - -1.7 4,009,274 - +0.0 4,009,449 + Pf1N1B4_3598 0.63 -2.9 4,009,449 + Pf1N1B4_3598 0.63 -3.1 4,009,450 - Pf1N1B4_3598 0.63 -2.3 4,009,450 - Pf1N1B4_3598 0.63 -2.9 4,009,450 - -1.7 4,009,451 + Pf1N1B4_3598 0.63 +0.0 4,009,451 + Pf1N1B4_3598 0.63 +0.0 4,009,451 + Pf1N1B4_3598 0.63 +0.0 4,009,451 + Pf1N1B4_3598 0.63 -1.7 4,009,452 - Pf1N1B4_3598 0.64 -3.6 4,009,561 + +0.0 4,009,583 + -2.7 4,009,583 + +0.0 4,009,583 + -4.7 4,009,583 + -0.7 4,009,622 + +0.0 4,009,622 + +0.0 4,009,681 + +0.0 4,009,886 + Pf1N1B4_3599 0.10 +0.0 4,009,886 + Pf1N1B4_3599 0.10 -2.1 4,009,887 - Pf1N1B4_3599 0.11 +0.0 4,009,887 - Pf1N1B4_3599 0.11 +0.0 4,009,887 - Pf1N1B4_3599 0.11 -1.8 4,009,887 - Pf1N1B4_3599 0.11 -2.2 4,009,941 - Pf1N1B4_3599 0.14 -3.9 4,010,062 + Pf1N1B4_3599 0.22 +0.0 4,010,113 + Pf1N1B4_3599 0.26 +0.0 4,010,113 + Pf1N1B4_3599 0.26 +0.0 4,010,113 + Pf1N1B4_3599 0.26 +0.0 4,010,113 + Pf1N1B4_3599 0.26 -4.2 4,010,346 + Pf1N1B4_3599 0.42 +0.0 4,010,451 + Pf1N1B4_3599 0.49 -3.5 4,010,641 + Pf1N1B4_3599 0.62 +0.0 4,010,641 + Pf1N1B4_3599 0.62 +0.0 4,010,642 - Pf1N1B4_3599 0.62 -2.8 4,011,163 + +0.0 4,011,164 - +0.0 4,011,232 + +0.0 4,011,233 - -2.0 4,011,234 + -2.2 4,011,234 + +0.0 4,011,234 + +0.0 4,011,264 + -1.8 4,011,264 + +0.0 4,011,264 + +0.0 4,011,453 + Pf1N1B4_3600 0.11 -2.2 4,011,629 + Pf1N1B4_3600 0.25 -2.4 4,011,758 - Pf1N1B4_3600 0.36 +0.0 4,011,882 - Pf1N1B4_3600 0.46 -2.1 4,011,936 + Pf1N1B4_3600 0.50 +0.0 4,011,937 - Pf1N1B4_3600 0.51 -0.4 4,012,191 - Pf1N1B4_3600 0.71 -2.8
Or see this region's nucleotide sequence