Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3118 and Pf1N1B4_3119 are separated by 521 nucleotides Pf1N1B4_3119 and Pf1N1B4_3120 are separated by 29 nucleotides Pf1N1B4_3120 and Pf1N1B4_3121 overlap by 35 nucleotides Pf1N1B4_3121 and Pf1N1B4_3122 overlap by 4 nucleotides Pf1N1B4_3122 and Pf1N1B4_3123 overlap by 1 nucleotides
Pf1N1B4_3118 - Transcriptional regulator, Cro/CI family, at 3,506,083 to 3,506,826
_3118
Pf1N1B4_3119 - Holin, at 3,507,348 to 3,507,695
_3119
Pf1N1B4_3120 - hypothetical protein, at 3,507,725 to 3,507,859
_3120
Pf1N1B4_3121 - FIG111678: IS, phage, Tn; Transposon-related functions, at 3,507,825 to 3,508,409
_3121
Pf1N1B4_3122 - Mu-like prophage FluMu protein GP38, at 3,508,406 to 3,508,588
_3122
Pf1N1B4_3123 - Bacteriophage tail sheath protein, at 3,508,588 to 3,510,084
_3123
Position (kb)
3507
3508 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3506.825 kb on - strand at 3506.843 kb on + strand at 3506.843 kb on + strand at 3506.843 kb on + strand at 3506.844 kb on - strand at 3506.844 kb on - strand at 3506.844 kb on - strand at 3507.030 kb on + strand at 3507.030 kb on + strand at 3507.031 kb on - strand at 3507.105 kb on + strand at 3507.105 kb on + strand at 3507.106 kb on - strand at 3507.106 kb on - strand at 3507.106 kb on - strand at 3507.213 kb on - strand at 3507.245 kb on + strand at 3507.305 kb on + strand at 3507.306 kb on - strand at 3507.541 kb on - strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.624 kb on + strand, within Pf1N1B4_3119 at 3507.625 kb on - strand at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.824 kb on + strand, within Pf1N1B4_3120 at 3507.825 kb on - strand at 3507.913 kb on + strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.914 kb on - strand, within Pf1N1B4_3121 at 3507.948 kb on + strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3507.949 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.100 kb on - strand, within Pf1N1B4_3121 at 3508.137 kb on + strand, within Pf1N1B4_3121 at 3508.152 kb on + strand, within Pf1N1B4_3121 at 3508.153 kb on - strand, within Pf1N1B4_3121 at 3508.399 kb on + strand at 3508.399 kb on + strand at 3508.587 kb on - strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.639 kb on + strand at 3508.640 kb on - strand at 3508.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 3,506,825 - -1.1 3,506,843 + -0.0 3,506,843 + -0.3 3,506,843 + -0.0 3,506,844 - -0.0 3,506,844 - -0.0 3,506,844 - -0.0 3,507,030 + -1.6 3,507,030 + -0.0 3,507,031 - -2.4 3,507,105 + -1.0 3,507,105 + -0.0 3,507,106 - -0.0 3,507,106 - -1.5 3,507,106 - -0.0 3,507,213 - -0.0 3,507,245 + -3.3 3,507,305 + -0.0 3,507,306 - -1.7 3,507,541 - Pf1N1B4_3119 0.55 -0.0 3,507,624 + Pf1N1B4_3119 0.79 -3.1 3,507,624 + Pf1N1B4_3119 0.79 -0.0 3,507,625 - -0.0 3,507,824 + Pf1N1B4_3120 0.73 -0.0 3,507,824 + Pf1N1B4_3120 0.73 -0.0 3,507,824 + Pf1N1B4_3120 0.73 -0.0 3,507,825 - -1.2 3,507,913 + Pf1N1B4_3121 0.15 -0.0 3,507,914 - Pf1N1B4_3121 0.15 -2.2 3,507,914 - Pf1N1B4_3121 0.15 -0.0 3,507,914 - Pf1N1B4_3121 0.15 -0.0 3,507,948 + Pf1N1B4_3121 0.21 -3.1 3,507,949 - Pf1N1B4_3121 0.21 -0.0 3,507,949 - Pf1N1B4_3121 0.21 -0.0 3,507,949 - Pf1N1B4_3121 0.21 -0.6 3,507,949 - Pf1N1B4_3121 0.21 -0.0 3,507,949 - Pf1N1B4_3121 0.21 -0.0 3,508,100 - Pf1N1B4_3121 0.47 -0.0 3,508,100 - Pf1N1B4_3121 0.47 -0.0 3,508,137 + Pf1N1B4_3121 0.53 -0.0 3,508,152 + Pf1N1B4_3121 0.56 -0.0 3,508,153 - Pf1N1B4_3121 0.56 -0.0 3,508,399 + -0.0 3,508,399 + -0.0 3,508,587 - -0.0 3,508,639 + -3.3 3,508,639 + -0.0 3,508,639 + -1.9 3,508,640 - -3.4 3,508,640 - -0.0
Or see this region's nucleotide sequence