Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2782 and Pf1N1B4_2783 are separated by 40 nucleotides Pf1N1B4_2783 and Pf1N1B4_2784 are separated by 70 nucleotides Pf1N1B4_2784 and Pf1N1B4_2785 are separated by 205 nucleotides
Pf1N1B4_2782 - Mobile element protein, at 3,126,989 to 3,128,131
_2782
Pf1N1B4_2783 - Transcription elongation factor GreB-related protein, at 3,128,172 to 3,128,666
_2783
Pf1N1B4_2784 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,128,737 to 3,129,093
_2784
Pf1N1B4_2785 - putative outermembrane protein, at 3,129,299 to 3,131,152
_2785
Position (kb)
3128
3129 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3127.796 kb on + strand, within Pf1N1B4_2782 at 3128.129 kb on + strand at 3128.129 kb on + strand at 3128.129 kb on + strand at 3128.138 kb on + strand at 3128.148 kb on + strand at 3128.148 kb on + strand at 3128.449 kb on + strand, within Pf1N1B4_2783 at 3128.581 kb on + strand, within Pf1N1B4_2783 at 3128.582 kb on - strand, within Pf1N1B4_2783 at 3128.582 kb on - strand, within Pf1N1B4_2783 at 3128.582 kb on - strand, within Pf1N1B4_2783 at 3128.639 kb on + strand at 3128.640 kb on - strand at 3128.682 kb on + strand at 3128.682 kb on + strand at 3128.682 kb on + strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.710 kb on - strand at 3128.710 kb on - strand at 3128.710 kb on - strand at 3128.729 kb on + strand at 3128.738 kb on + strand at 3128.738 kb on + strand at 3128.738 kb on + strand at 3128.738 kb on + strand at 3128.822 kb on + strand, within Pf1N1B4_2784 at 3128.822 kb on + strand, within Pf1N1B4_2784 at 3128.823 kb on - strand, within Pf1N1B4_2784 at 3128.823 kb on - strand, within Pf1N1B4_2784 at 3128.892 kb on - strand, within Pf1N1B4_2784 at 3128.892 kb on - strand, within Pf1N1B4_2784 at 3128.910 kb on - strand, within Pf1N1B4_2784 at 3129.123 kb on + strand at 3129.134 kb on + strand at 3129.134 kb on + strand at 3129.135 kb on - strand at 3129.135 kb on - strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.174 kb on + strand at 3129.175 kb on - strand at 3129.303 kb on + strand at 3129.303 kb on + strand at 3129.309 kb on + strand at 3129.310 kb on - strand at 3129.326 kb on + strand at 3129.326 kb on + strand at 3129.326 kb on + strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.456 kb on + strand at 3129.456 kb on + strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.503 kb on + strand, within Pf1N1B4_2785 at 3129.504 kb on - strand, within Pf1N1B4_2785 at 3129.559 kb on + strand, within Pf1N1B4_2785 at 3129.559 kb on + strand, within Pf1N1B4_2785 at 3129.559 kb on + strand, within Pf1N1B4_2785 at 3129.560 kb on - strand, within Pf1N1B4_2785 at 3129.560 kb on - strand, within Pf1N1B4_2785 at 3129.598 kb on + strand, within Pf1N1B4_2785 at 3129.599 kb on - strand at 3129.599 kb on - strand, within Pf1N1B4_2785
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 3,127,796 + Pf1N1B4_2782 0.71 -0.0 3,128,129 + -1.3 3,128,129 + -2.0 3,128,129 + -0.0 3,128,138 + -0.0 3,128,148 + -2.0 3,128,148 + -0.0 3,128,449 + Pf1N1B4_2783 0.56 -0.0 3,128,581 + Pf1N1B4_2783 0.83 -0.0 3,128,582 - Pf1N1B4_2783 0.83 -3.7 3,128,582 - Pf1N1B4_2783 0.83 -0.0 3,128,582 - Pf1N1B4_2783 0.83 -0.0 3,128,639 + -0.0 3,128,640 - -0.3 3,128,682 + -0.7 3,128,682 + -0.0 3,128,682 + -2.8 3,128,683 - -0.0 3,128,683 - -1.8 3,128,683 - -0.0 3,128,683 - +0.1 3,128,683 - -0.0 3,128,710 - -1.9 3,128,710 - -0.0 3,128,710 - -0.0 3,128,729 + -0.0 3,128,738 + -3.1 3,128,738 + -1.1 3,128,738 + -0.0 3,128,738 + -0.0 3,128,822 + Pf1N1B4_2784 0.24 -0.0 3,128,822 + Pf1N1B4_2784 0.24 -0.0 3,128,823 - Pf1N1B4_2784 0.24 -0.0 3,128,823 - Pf1N1B4_2784 0.24 -0.0 3,128,892 - Pf1N1B4_2784 0.43 -0.0 3,128,892 - Pf1N1B4_2784 0.43 -0.0 3,128,910 - Pf1N1B4_2784 0.48 -1.4 3,129,123 + -3.7 3,129,134 + -0.0 3,129,134 + -0.0 3,129,135 - -2.8 3,129,135 - -2.0 3,129,155 + -0.0 3,129,155 + -1.3 3,129,155 + -0.0 3,129,155 + -0.0 3,129,155 + -0.0 3,129,155 + -0.0 3,129,155 + -0.0 3,129,155 + -2.1 3,129,155 + -2.4 3,129,155 + -1.6 3,129,155 + -0.0 3,129,156 - -0.0 3,129,156 - -0.0 3,129,156 - -2.5 3,129,156 - -2.1 3,129,156 - -0.0 3,129,156 - -0.0 3,129,156 - -0.0 3,129,156 - -0.0 3,129,174 + -2.4 3,129,175 - -0.0 3,129,303 + -0.0 3,129,303 + -0.0 3,129,309 + -2.4 3,129,310 - -0.5 3,129,326 + -0.0 3,129,326 + -1.5 3,129,326 + -0.0 3,129,327 - -0.0 3,129,327 - -0.0 3,129,327 - -2.5 3,129,327 - -0.0 3,129,327 - -0.0 3,129,456 + -0.0 3,129,456 + -0.0 3,129,457 - -0.0 3,129,457 - -0.0 3,129,457 - -1.7 3,129,457 - -0.0 3,129,457 - -0.0 3,129,457 - -0.0 3,129,457 - -0.0 3,129,503 + Pf1N1B4_2785 0.11 -0.0 3,129,504 - Pf1N1B4_2785 0.11 -0.0 3,129,559 + Pf1N1B4_2785 0.14 -0.0 3,129,559 + Pf1N1B4_2785 0.14 -1.4 3,129,559 + Pf1N1B4_2785 0.14 -0.0 3,129,560 - Pf1N1B4_2785 0.14 -0.0 3,129,560 - Pf1N1B4_2785 0.14 -0.3 3,129,598 + Pf1N1B4_2785 0.16 -0.0 3,129,599 - -1.6 3,129,599 - Pf1N1B4_2785 0.16 -1.6
Or see this region's nucleotide sequence