Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2716 and Pf1N1B4_2717 are separated by 18 nucleotides Pf1N1B4_2717 and Pf1N1B4_2718 are separated by 18 nucleotides Pf1N1B4_2718 and Pf1N1B4_2719 are separated by 496 nucleotides
Pf1N1B4_2716 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 3,052,430 to 3,053,713
_2716
Pf1N1B4_2717 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 3,053,732 to 3,054,355
_2717
Pf1N1B4_2718 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 3,054,374 to 3,055,102
_2718
Pf1N1B4_2719 - Sensor histidine kinase, at 3,055,599 to 3,057,983
_2719
Position (kb)
3054
3055
3056 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3054.315 kb on - strand at 3054.347 kb on + strand at 3054.348 kb on - strand at 3054.348 kb on - strand at 3054.348 kb on - strand at 3054.348 kb on - strand at 3054.460 kb on - strand, within Pf1N1B4_2718 at 3054.460 kb on - strand at 3054.460 kb on - strand, within Pf1N1B4_2718 at 3054.460 kb on - strand, within Pf1N1B4_2718 at 3054.552 kb on + strand, within Pf1N1B4_2718 at 3054.552 kb on + strand, within Pf1N1B4_2718 at 3054.552 kb on + strand, within Pf1N1B4_2718 at 3054.553 kb on - strand, within Pf1N1B4_2718 at 3054.606 kb on + strand, within Pf1N1B4_2718 at 3054.606 kb on + strand, within Pf1N1B4_2718 at 3054.606 kb on + strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.621 kb on - strand, within Pf1N1B4_2718 at 3055.091 kb on - strand at 3055.117 kb on + strand at 3055.117 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.161 kb on + strand at 3055.162 kb on - strand at 3055.162 kb on - strand at 3055.162 kb on - strand at 3055.162 kb on - strand at 3055.162 kb on - strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.165 kb on + strand at 3055.166 kb on - strand at 3055.166 kb on - strand at 3055.166 kb on - strand at 3055.185 kb on - strand at 3055.231 kb on + strand at 3055.231 kb on + strand at 3055.232 kb on - strand at 3055.232 kb on - strand at 3055.360 kb on + strand at 3055.361 kb on - strand at 3055.410 kb on + strand at 3055.410 kb on + strand at 3055.410 kb on + strand at 3055.420 kb on + strand at 3055.420 kb on + strand at 3055.420 kb on + strand at 3055.420 kb on + strand at 3055.420 kb on + strand at 3055.420 kb on + strand at 3055.420 kb on + strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.421 kb on - strand at 3055.422 kb on + strand at 3055.423 kb on - strand at 3055.423 kb on - strand at 3055.443 kb on - strand at 3055.492 kb on + strand at 3055.492 kb on + strand at 3055.497 kb on + strand at 3055.497 kb on + strand at 3055.497 kb on + strand at 3055.498 kb on - strand at 3055.498 kb on - strand at 3055.498 kb on - strand at 3055.498 kb on - strand at 3055.523 kb on + strand at 3055.523 kb on + strand at 3055.524 kb on - strand at 3055.524 kb on - strand at 3055.524 kb on - strand at 3055.524 kb on - strand at 3055.524 kb on - strand at 3055.535 kb on + strand at 3055.535 kb on + strand at 3055.536 kb on - strand at 3055.541 kb on + strand at 3055.541 kb on + strand at 3055.596 kb on + strand at 3055.597 kb on - strand at 3055.597 kb on - strand at 3055.597 kb on - strand at 3055.704 kb on + strand at 3055.704 kb on + strand at 3055.705 kb on - strand at 3055.782 kb on - strand at 3055.920 kb on + strand, within Pf1N1B4_2719 at 3055.920 kb on + strand, within Pf1N1B4_2719 at 3055.920 kb on + strand, within Pf1N1B4_2719 at 3056.010 kb on + strand, within Pf1N1B4_2719 at 3056.010 kb on + strand, within Pf1N1B4_2719 at 3056.011 kb on - strand, within Pf1N1B4_2719 at 3056.011 kb on - strand, within Pf1N1B4_2719 at 3056.011 kb on - strand at 3056.055 kb on + strand, within Pf1N1B4_2719 at 3056.096 kb on + strand, within Pf1N1B4_2719 at 3056.096 kb on + strand, within Pf1N1B4_2719 at 3056.097 kb on - strand, within Pf1N1B4_2719
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 3,053,989 - Pf1N1B4_2717 0.41 -3.7 3,053,989 - Pf1N1B4_2717 0.41 -4.9 3,053,989 - Pf1N1B4_2717 0.41 -0.0 3,053,989 - Pf1N1B4_2717 0.41 -0.0 3,054,315 - -0.0 3,054,347 + -0.0 3,054,348 - -0.0 3,054,348 - -0.0 3,054,348 - -0.0 3,054,348 - -0.0 3,054,460 - Pf1N1B4_2718 0.12 -0.0 3,054,460 - -0.0 3,054,460 - Pf1N1B4_2718 0.12 -2.7 3,054,460 - Pf1N1B4_2718 0.12 -0.0 3,054,552 + Pf1N1B4_2718 0.24 -0.0 3,054,552 + Pf1N1B4_2718 0.24 -2.4 3,054,552 + Pf1N1B4_2718 0.24 -0.0 3,054,553 - Pf1N1B4_2718 0.25 -0.0 3,054,606 + Pf1N1B4_2718 0.32 -0.0 3,054,606 + Pf1N1B4_2718 0.32 -0.0 3,054,606 + Pf1N1B4_2718 0.32 -0.0 3,054,607 - Pf1N1B4_2718 0.32 -3.7 3,054,607 - Pf1N1B4_2718 0.32 -4.2 3,054,607 - Pf1N1B4_2718 0.32 -0.0 3,054,607 - Pf1N1B4_2718 0.32 -0.0 3,054,620 + Pf1N1B4_2718 0.34 -3.2 3,054,620 + Pf1N1B4_2718 0.34 -0.0 3,054,620 + Pf1N1B4_2718 0.34 -0.0 3,054,620 + Pf1N1B4_2718 0.34 -5.1 3,054,620 + Pf1N1B4_2718 0.34 -0.0 3,054,620 + Pf1N1B4_2718 0.34 -0.0 3,054,620 + -1.5 3,054,620 + Pf1N1B4_2718 0.34 -0.0 3,054,621 - Pf1N1B4_2718 0.34 -2.3 3,055,091 - -0.0 3,055,117 + -0.0 3,055,117 + -3.2 3,055,161 + -0.0 3,055,161 + -0.0 3,055,161 + -0.0 3,055,161 + -2.6 3,055,161 + -0.0 3,055,161 + -3.2 3,055,161 + -3.3 3,055,161 + -1.0 3,055,161 + -0.0 3,055,161 + -1.6 3,055,161 + -1.0 3,055,161 + -0.0 3,055,161 + -0.0 3,055,161 + -0.0 3,055,161 + -2.3 3,055,162 - -0.0 3,055,162 - -0.0 3,055,162 - -0.0 3,055,162 - -0.0 3,055,162 - -4.1 3,055,165 + -0.0 3,055,165 + -0.0 3,055,165 + -2.3 3,055,165 + -2.3 3,055,165 + -0.0 3,055,165 + -0.0 3,055,165 + -0.0 3,055,165 + -0.0 3,055,165 + -0.0 3,055,165 + -0.0 3,055,166 - -0.0 3,055,166 - -1.4 3,055,166 - -0.0 3,055,185 - -2.1 3,055,231 + -0.0 3,055,231 + +1.8 3,055,232 - -0.0 3,055,232 - -0.0 3,055,360 + -0.0 3,055,361 - -0.0 3,055,410 + -0.0 3,055,410 + -0.7 3,055,410 + -0.0 3,055,420 + -3.1 3,055,420 + -1.6 3,055,420 + -0.0 3,055,420 + -2.5 3,055,420 + -1.5 3,055,420 + -0.0 3,055,420 + -0.0 3,055,421 - -2.7 3,055,421 - -0.0 3,055,421 - -0.7 3,055,421 - -1.8 3,055,421 - -1.1 3,055,421 - -1.4 3,055,421 - -2.0 3,055,421 - -1.6 3,055,421 - -0.9 3,055,421 - -1.4 3,055,421 - -0.0 3,055,422 + -0.0 3,055,423 - -1.5 3,055,423 - -1.9 3,055,443 - -1.0 3,055,492 + -0.0 3,055,492 + -0.0 3,055,497 + -0.0 3,055,497 + -0.0 3,055,497 + -0.0 3,055,498 - -0.0 3,055,498 - -1.0 3,055,498 - -1.8 3,055,498 - -0.0 3,055,523 + -0.0 3,055,523 + -3.5 3,055,524 - -0.8 3,055,524 - -0.0 3,055,524 - -0.0 3,055,524 - -1.1 3,055,524 - -0.9 3,055,535 + -1.7 3,055,535 + -1.3 3,055,536 - -0.0 3,055,541 + -0.0 3,055,541 + -0.0 3,055,596 + -0.0 3,055,597 - -0.0 3,055,597 - -0.0 3,055,597 - -0.0 3,055,704 + -0.0 3,055,704 + -0.0 3,055,705 - -0.6 3,055,782 - -0.0 3,055,920 + Pf1N1B4_2719 0.13 -0.0 3,055,920 + Pf1N1B4_2719 0.13 -0.0 3,055,920 + Pf1N1B4_2719 0.13 -0.0 3,056,010 + Pf1N1B4_2719 0.17 -0.0 3,056,010 + Pf1N1B4_2719 0.17 -0.0 3,056,011 - Pf1N1B4_2719 0.17 -0.0 3,056,011 - Pf1N1B4_2719 0.17 -0.0 3,056,011 - -0.0 3,056,055 + Pf1N1B4_2719 0.19 -0.0 3,056,096 + Pf1N1B4_2719 0.21 -0.0 3,056,096 + Pf1N1B4_2719 0.21 -0.0 3,056,097 - Pf1N1B4_2719 0.21 -1.1
Or see this region's nucleotide sequence