Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2651

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2649 and Pf1N1B4_2650 are separated by 47 nucleotidesPf1N1B4_2650 and Pf1N1B4_2651 are separated by 31 nucleotidesPf1N1B4_2651 and Pf1N1B4_2652 are separated by 91 nucleotidesPf1N1B4_2652 and Pf1N1B4_2653 overlap by 4 nucleotides Pf1N1B4_2649 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 2,986,677 to 2,987,810 _2649 Pf1N1B4_2650 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 2,987,858 to 2,988,520 _2650 Pf1N1B4_2651 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 2,988,552 to 2,989,643 _2651 Pf1N1B4_2652 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 2,989,735 to 2,990,211 _2652 Pf1N1B4_2653 - Transcription termination protein NusB, at 2,990,208 to 2,990,708 _2653 Position (kb) 2988 2989 2990Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2987.806 kb on + strandat 2987.806 kb on + strandat 2987.806 kb on + strandat 2987.807 kb on - strandat 2987.807 kb on - strandat 2987.807 kb on - strandat 2987.808 kb on + strandat 2987.808 kb on + strandat 2987.808 kb on + strandat 2987.808 kb on + strandat 2988.563 kb on + strandat 2988.845 kb on + strand, within Pf1N1B4_2651at 2989.163 kb on + strand, within Pf1N1B4_2651at 2989.163 kb on + strand, within Pf1N1B4_2651at 2989.163 kb on + strand, within Pf1N1B4_2651at 2989.227 kb on + strand, within Pf1N1B4_2651at 2989.227 kb on + strand, within Pf1N1B4_2651at 2989.228 kb on - strand, within Pf1N1B4_2651at 2989.228 kb on - strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strandat 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.447 kb on + strand, within Pf1N1B4_2651at 2989.506 kb on + strand, within Pf1N1B4_2651at 2989.506 kb on + strand, within Pf1N1B4_2651at 2989.506 kb on + strand, within Pf1N1B4_2651at 2989.506 kb on + strand, within Pf1N1B4_2651at 2989.506 kb on + strand, within Pf1N1B4_2651at 2989.589 kb on + strandat 2989.621 kb on + strandat 2989.626 kb on + strandat 2989.626 kb on + strandat 2989.626 kb on + strandat 2989.626 kb on + strandat 2989.698 kb on + strandat 2989.698 kb on + strandat 2989.720 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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2,987,806 + -1.8
2,987,806 + -0.0
2,987,806 + -0.0
2,987,807 - -0.0
2,987,807 - +2.1
2,987,807 - -1.6
2,987,808 + -0.0
2,987,808 + -0.0
2,987,808 + -0.0
2,987,808 + -0.0
2,988,563 + -0.0
2,988,845 + Pf1N1B4_2651 0.27 -0.0
2,989,163 + Pf1N1B4_2651 0.56 -0.0
2,989,163 + Pf1N1B4_2651 0.56 -0.0
2,989,163 + Pf1N1B4_2651 0.56 -4.1
2,989,227 + Pf1N1B4_2651 0.62 -3.1
2,989,227 + Pf1N1B4_2651 0.62 -0.0
2,989,228 - Pf1N1B4_2651 0.62 -0.0
2,989,228 - Pf1N1B4_2651 0.62 -3.3
2,989,447 + Pf1N1B4_2651 0.82 -3.4
2,989,447 + Pf1N1B4_2651 0.82 -0.0
2,989,447 + Pf1N1B4_2651 0.82 -0.0
2,989,447 + Pf1N1B4_2651 0.82 -0.0
2,989,447 + Pf1N1B4_2651 0.82 -3.6
2,989,447 + -3.0
2,989,447 + Pf1N1B4_2651 0.82 -0.0
2,989,447 + Pf1N1B4_2651 0.82 -0.0
2,989,447 + Pf1N1B4_2651 0.82 -0.0
2,989,506 + Pf1N1B4_2651 0.87 -3.9
2,989,506 + Pf1N1B4_2651 0.87 -0.0
2,989,506 + Pf1N1B4_2651 0.87 -0.0
2,989,506 + Pf1N1B4_2651 0.87 -0.0
2,989,506 + Pf1N1B4_2651 0.87 -0.0
2,989,589 + -0.0
2,989,621 + -4.0
2,989,626 + -0.0
2,989,626 + -0.0
2,989,626 + -2.3
2,989,626 + -1.1
2,989,698 + -0.0
2,989,698 + -0.0
2,989,720 + -0.0

Or see this region's nucleotide sequence