Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2649 and Pf1N1B4_2650 are separated by 47 nucleotides Pf1N1B4_2650 and Pf1N1B4_2651 are separated by 31 nucleotides Pf1N1B4_2651 and Pf1N1B4_2652 are separated by 91 nucleotides Pf1N1B4_2652 and Pf1N1B4_2653 overlap by 4 nucleotides
Pf1N1B4_2649 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 2,986,677 to 2,987,810
_2649
Pf1N1B4_2650 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 2,987,858 to 2,988,520
_2650
Pf1N1B4_2651 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 2,988,552 to 2,989,643
_2651
Pf1N1B4_2652 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 2,989,735 to 2,990,211
_2652
Pf1N1B4_2653 - Transcription termination protein NusB, at 2,990,208 to 2,990,708
_2653
Position (kb)
2988
2989
2990 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2987.806 kb on + strand at 2987.806 kb on + strand at 2987.806 kb on + strand at 2987.807 kb on - strand at 2987.807 kb on - strand at 2987.807 kb on - strand at 2987.808 kb on + strand at 2987.808 kb on + strand at 2987.808 kb on + strand at 2987.808 kb on + strand at 2988.563 kb on + strand at 2988.845 kb on + strand, within Pf1N1B4_2651 at 2989.163 kb on + strand, within Pf1N1B4_2651 at 2989.163 kb on + strand, within Pf1N1B4_2651 at 2989.163 kb on + strand, within Pf1N1B4_2651 at 2989.227 kb on + strand, within Pf1N1B4_2651 at 2989.227 kb on + strand, within Pf1N1B4_2651 at 2989.228 kb on - strand, within Pf1N1B4_2651 at 2989.228 kb on - strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.447 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.506 kb on + strand, within Pf1N1B4_2651 at 2989.589 kb on + strand at 2989.621 kb on + strand at 2989.626 kb on + strand at 2989.626 kb on + strand at 2989.626 kb on + strand at 2989.626 kb on + strand at 2989.698 kb on + strand at 2989.698 kb on + strand at 2989.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,987,806 + -1.8 2,987,806 + -0.0 2,987,806 + -0.0 2,987,807 - -0.0 2,987,807 - +2.1 2,987,807 - -1.6 2,987,808 + -0.0 2,987,808 + -0.0 2,987,808 + -0.0 2,987,808 + -0.0 2,988,563 + -0.0 2,988,845 + Pf1N1B4_2651 0.27 -0.0 2,989,163 + Pf1N1B4_2651 0.56 -0.0 2,989,163 + Pf1N1B4_2651 0.56 -0.0 2,989,163 + Pf1N1B4_2651 0.56 -4.1 2,989,227 + Pf1N1B4_2651 0.62 -3.1 2,989,227 + Pf1N1B4_2651 0.62 -0.0 2,989,228 - Pf1N1B4_2651 0.62 -0.0 2,989,228 - Pf1N1B4_2651 0.62 -3.3 2,989,447 + Pf1N1B4_2651 0.82 -3.4 2,989,447 + Pf1N1B4_2651 0.82 -0.0 2,989,447 + Pf1N1B4_2651 0.82 -0.0 2,989,447 + Pf1N1B4_2651 0.82 -0.0 2,989,447 + Pf1N1B4_2651 0.82 -3.6 2,989,447 + -3.0 2,989,447 + Pf1N1B4_2651 0.82 -0.0 2,989,447 + Pf1N1B4_2651 0.82 -0.0 2,989,447 + Pf1N1B4_2651 0.82 -0.0 2,989,506 + Pf1N1B4_2651 0.87 -3.9 2,989,506 + Pf1N1B4_2651 0.87 -0.0 2,989,506 + Pf1N1B4_2651 0.87 -0.0 2,989,506 + Pf1N1B4_2651 0.87 -0.0 2,989,506 + Pf1N1B4_2651 0.87 -0.0 2,989,589 + -0.0 2,989,621 + -4.0 2,989,626 + -0.0 2,989,626 + -0.0 2,989,626 + -2.3 2,989,626 + -1.1 2,989,698 + -0.0 2,989,698 + -0.0 2,989,720 + -0.0
Or see this region's nucleotide sequence