Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2512 and Pf1N1B4_2513 are separated by 122 nucleotides Pf1N1B4_2513 and Pf1N1B4_2514 are separated by 67 nucleotides
Pf1N1B4_2512 - sensory box/GGDEF domain/EAL domain protein, at 2,847,041 to 2,850,787
_2512
Pf1N1B4_2513 - RNA polymerase sigma factor RpoD, at 2,850,910 to 2,852,757
_2513
Pf1N1B4_2514 - DNA primase (EC 2.7.7.-), at 2,852,825 to 2,854,798
_2514
Position (kb)
2850
2851
2852
2853 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2849.960 kb on + strand, within Pf1N1B4_2512 at 2849.976 kb on + strand at 2849.977 kb on - strand, within Pf1N1B4_2512 at 2849.977 kb on - strand, within Pf1N1B4_2512 at 2850.162 kb on + strand, within Pf1N1B4_2512 at 2850.162 kb on + strand, within Pf1N1B4_2512 at 2850.162 kb on + strand, within Pf1N1B4_2512 at 2850.162 kb on + strand, within Pf1N1B4_2512 at 2850.163 kb on - strand, within Pf1N1B4_2512 at 2850.219 kb on - strand, within Pf1N1B4_2512 at 2850.222 kb on - strand, within Pf1N1B4_2512 at 2850.222 kb on - strand, within Pf1N1B4_2512 at 2850.314 kb on + strand, within Pf1N1B4_2512 at 2850.314 kb on + strand, within Pf1N1B4_2512 at 2850.314 kb on + strand, within Pf1N1B4_2512 at 2850.315 kb on - strand, within Pf1N1B4_2512 at 2850.315 kb on - strand, within Pf1N1B4_2512 at 2850.330 kb on + strand, within Pf1N1B4_2512 at 2850.331 kb on - strand, within Pf1N1B4_2512 at 2850.361 kb on - strand, within Pf1N1B4_2512 at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.450 kb on + strand at 2850.451 kb on - strand at 2850.451 kb on - strand at 2850.451 kb on - strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.475 kb on + strand at 2850.476 kb on - strand at 2850.476 kb on - strand at 2850.494 kb on + strand at 2850.494 kb on + strand at 2850.494 kb on + strand at 2850.494 kb on + strand at 2850.494 kb on + strand at 2850.494 kb on + strand at 2850.494 kb on + strand at 2850.697 kb on + strand at 2850.697 kb on + strand at 2850.697 kb on + strand at 2850.697 kb on + strand at 2850.698 kb on - strand at 2850.698 kb on - strand at 2850.698 kb on - strand at 2850.698 kb on - strand at 2850.762 kb on + strand at 2850.763 kb on - strand at 2850.763 kb on - strand at 2850.763 kb on - strand at 2850.763 kb on - strand at 2850.826 kb on - strand at 2850.826 kb on - strand at 2850.852 kb on + strand at 2850.852 kb on + strand at 2850.852 kb on + strand at 2850.853 kb on - strand at 2850.853 kb on - strand at 2850.853 kb on - strand at 2850.863 kb on + strand at 2850.863 kb on + strand at 2850.863 kb on + strand at 2850.863 kb on + strand at 2850.863 kb on + strand at 2850.863 kb on + strand at 2850.863 kb on + strand at 2850.864 kb on - strand at 2850.864 kb on - strand at 2850.873 kb on - strand at 2852.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,849,960 + Pf1N1B4_2512 0.78 -1.7 2,849,976 + -0.0 2,849,977 - Pf1N1B4_2512 0.78 -0.9 2,849,977 - Pf1N1B4_2512 0.78 -0.0 2,850,162 + Pf1N1B4_2512 0.83 -0.0 2,850,162 + Pf1N1B4_2512 0.83 -0.0 2,850,162 + Pf1N1B4_2512 0.83 -1.4 2,850,162 + Pf1N1B4_2512 0.83 -1.2 2,850,163 - Pf1N1B4_2512 0.83 -4.4 2,850,219 - Pf1N1B4_2512 0.85 -0.0 2,850,222 - Pf1N1B4_2512 0.85 -0.0 2,850,222 - Pf1N1B4_2512 0.85 -0.0 2,850,314 + Pf1N1B4_2512 0.87 -0.0 2,850,314 + Pf1N1B4_2512 0.87 -0.0 2,850,314 + Pf1N1B4_2512 0.87 -0.0 2,850,315 - Pf1N1B4_2512 0.87 -0.0 2,850,315 - Pf1N1B4_2512 0.87 -0.0 2,850,330 + Pf1N1B4_2512 0.88 -0.0 2,850,331 - Pf1N1B4_2512 0.88 -0.0 2,850,361 - Pf1N1B4_2512 0.89 -0.0 2,850,450 + -0.0 2,850,450 + -0.0 2,850,450 + -0.0 2,850,450 + -0.0 2,850,450 + -0.0 2,850,450 + -0.0 2,850,450 + -1.7 2,850,450 + -0.3 2,850,451 - -0.0 2,850,451 - -1.0 2,850,451 - -0.0 2,850,475 + -0.7 2,850,475 + -0.0 2,850,475 + -0.0 2,850,475 + -0.0 2,850,475 + -0.0 2,850,475 + -0.0 2,850,475 + -0.0 2,850,475 + -0.0 2,850,475 + -1.7 2,850,476 - -0.8 2,850,476 - -0.0 2,850,494 + -0.1 2,850,494 + -0.0 2,850,494 + -0.0 2,850,494 + -0.0 2,850,494 + -0.0 2,850,494 + -0.0 2,850,494 + -0.0 2,850,697 + -1.9 2,850,697 + -1.8 2,850,697 + -0.0 2,850,697 + -0.0 2,850,698 - -0.0 2,850,698 - -0.0 2,850,698 - -0.0 2,850,698 - -0.0 2,850,762 + -2.4 2,850,763 - -0.0 2,850,763 - -0.6 2,850,763 - -0.0 2,850,763 - +1.0 2,850,826 - -2.3 2,850,826 - -0.0 2,850,852 + -0.0 2,850,852 + -0.0 2,850,852 + -3.2 2,850,853 - -2.5 2,850,853 - -3.5 2,850,853 - -0.0 2,850,863 + -0.0 2,850,863 + -0.0 2,850,863 + -0.0 2,850,863 + -1.0 2,850,863 + -2.7 2,850,863 + -0.0 2,850,863 + -0.0 2,850,864 - -0.0 2,850,864 - -0.9 2,850,873 - -3.0 2,852,810 - +0.2
Or see this region's nucleotide sequence