Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_235 and Pf1N1B4_236 are separated by 22 nucleotides Pf1N1B4_236 and Pf1N1B4_237 are separated by 81 nucleotides Pf1N1B4_237 and Pf1N1B4_238 are separated by 76 nucleotides
Pf1N1B4_235 - Molybdopterin biosynthesis protein MoeA, at 295,218 to 296,405
_235
Pf1N1B4_236 - Molybdenum cofactor biosynthesis protein MoaB, at 296,428 to 296,967
_236
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Pf1N1B4_238 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 297,728 to 297,949
_238
Position (kb) 296
297 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 295.483 kb on + strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.735 kb on - strand, within Pf1N1B4_235 at 295.777 kb on - strand, within Pf1N1B4_235 at 295.812 kb on - strand, within Pf1N1B4_235 at 295.819 kb on + strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.820 kb on - strand, within Pf1N1B4_235 at 295.949 kb on + strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.053 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.148 kb on - strand, within Pf1N1B4_235 at 296.240 kb on - strand, within Pf1N1B4_235 at 296.638 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.821 kb on + strand, within Pf1N1B4_236 at 296.838 kb on + strand, within Pf1N1B4_236 at 296.884 kb on - strand, within Pf1N1B4_236 at 296.947 kb on + strand at 296.948 kb on - strand at 296.948 kb on - strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.967 kb on + strand at 296.968 kb on - strand at 296.968 kb on - strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.983 kb on + strand at 296.984 kb on - strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.003 kb on + strand at 297.004 kb on - strand at 297.004 kb on - strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.005 kb on + strand at 297.006 kb on - strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.609 kb on + strand at 297.623 kb on + strand at 297.677 kb on + strand at 297.677 kb on + strand at 297.678 kb on - strand at 297.678 kb on - strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.680 kb on - strand at 297.800 kb on - strand at 297.947 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 295,483 + Pf1N1B4_235 0.22 -1.6 295,735 - Pf1N1B4_235 0.44 -2.0 295,735 - Pf1N1B4_235 0.44 -2.6 295,735 - Pf1N1B4_235 0.44 -0.7 295,777 - Pf1N1B4_235 0.47 -2.2 295,812 - Pf1N1B4_235 0.50 -0.0 295,819 + Pf1N1B4_235 0.51 -0.0 295,820 - Pf1N1B4_235 0.51 -0.0 295,820 - Pf1N1B4_235 0.51 -0.0 295,820 - Pf1N1B4_235 0.51 -0.0 295,820 - Pf1N1B4_235 0.51 -3.5 295,820 - Pf1N1B4_235 0.51 -0.9 295,949 + Pf1N1B4_235 0.62 -2.4 296,053 - Pf1N1B4_235 0.70 -1.6 296,053 - Pf1N1B4_235 0.70 -0.0 296,148 - Pf1N1B4_235 0.78 -0.0 296,148 - Pf1N1B4_235 0.78 -0.5 296,240 - Pf1N1B4_235 0.86 -0.0 296,638 + Pf1N1B4_236 0.39 -0.0 296,821 + Pf1N1B4_236 0.73 -3.2 296,821 + Pf1N1B4_236 0.73 -0.0 296,821 + Pf1N1B4_236 0.73 -0.0 296,838 + Pf1N1B4_236 0.76 -2.1 296,884 - Pf1N1B4_236 0.84 -2.4 296,947 + -0.0 296,948 - -0.0 296,948 - -0.0 296,967 + -0.0 296,967 + -2.8 296,967 + -1.8 296,968 - -0.0 296,968 - -1.8 296,983 + -0.0 296,983 + -1.6 296,983 + -1.0 296,984 - -1.2 297,003 + -0.0 297,003 + -1.1 297,003 + -2.9 297,003 + -0.0 297,003 + -0.0 297,003 + -3.4 297,003 + -0.0 297,003 + -0.0 297,003 + -1.5 297,004 - -0.0 297,004 - -0.0 297,005 + -0.0 297,005 + -0.0 297,005 + -0.0 297,006 - -0.0 297,045 + -0.0 297,045 + -2.0 297,045 + -0.0 297,045 + -0.0 297,045 + -1.3 297,046 - -0.0 297,046 - -0.0 297,060 + -0.0 297,060 + -0.0 297,061 - -0.0 297,061 - -4.0 297,061 - -4.3 297,238 + Pf1N1B4_237 0.31 -0.0 297,239 - Pf1N1B4_237 0.32 -0.0 297,239 - Pf1N1B4_237 0.32 -0.0 297,239 - Pf1N1B4_237 0.32 -0.0 297,341 + Pf1N1B4_237 0.48 -3.0 297,341 + Pf1N1B4_237 0.48 -0.0 297,341 + Pf1N1B4_237 0.48 -0.0 297,342 - Pf1N1B4_237 0.49 -4.3 297,543 + Pf1N1B4_237 0.82 -0.0 297,543 + Pf1N1B4_237 0.82 -0.0 297,609 + -0.0 297,623 + -0.0 297,677 + -0.7 297,677 + -1.1 297,678 - -0.0 297,678 - -0.0 297,679 + -0.0 297,679 + -0.0 297,679 + -0.0 297,679 + -0.0 297,679 + -0.0 297,679 + -1.4 297,679 + -2.0 297,679 + -0.0 297,680 - -0.0 297,800 - -0.0 297,947 + -0.0
Or see this region's nucleotide sequence