Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotides Pf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotides Pf1N1B4_2337 and Pf1N1B4_2338 are separated by 7 nucleotides
Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644
_2335
Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075
_2336
Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722
_2337
Pf1N1B4_2338 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,730 to 2,655,869
_2338
Position (kb)
2654
2655 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.759 kb on + strand, within Pf1N1B4_2336 at 2653.856 kb on + strand at 2653.857 kb on - strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2654.048 kb on + strand at 2654.049 kb on - strand at 2654.049 kb on - strand at 2654.052 kb on + strand at 2654.052 kb on + strand at 2654.053 kb on - strand at 2654.064 kb on + strand at 2654.064 kb on + strand at 2654.165 kb on + strand, within Pf1N1B4_2337 at 2654.174 kb on - strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.308 kb on - strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.451 kb on + strand, within Pf1N1B4_2337 at 2654.739 kb on + strand at 2654.739 kb on + strand at 2654.741 kb on + strand at 2654.741 kb on + strand at 2654.742 kb on - strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.752 kb on + strand at 2654.753 kb on - strand at 2654.753 kb on - strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.773 kb on + strand at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2654.993 kb on - strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.019 kb on + strand, within Pf1N1B4_2338 at 2655.020 kb on - strand, within Pf1N1B4_2338 at 2655.020 kb on - strand, within Pf1N1B4_2338 at 2655.062 kb on - strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.569 kb on + strand at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.570 kb on - strand, within Pf1N1B4_2338
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,653,125 + Pf1N1B4_2335 0.13 -0.0 2,653,125 + Pf1N1B4_2335 0.13 -1.3 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,126 - Pf1N1B4_2335 0.13 -0.0 2,653,389 - Pf1N1B4_2335 0.57 -2.6 2,653,389 - Pf1N1B4_2335 0.57 -1.7 2,653,389 - Pf1N1B4_2335 0.57 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -2.2 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + -2.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,757 - Pf1N1B4_2336 0.27 -0.0 2,653,757 - Pf1N1B4_2336 0.27 -0.0 2,653,759 + Pf1N1B4_2336 0.27 -2.8 2,653,856 + -0.7 2,653,857 - Pf1N1B4_2336 0.50 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -2.4 2,653,963 + Pf1N1B4_2336 0.74 -1.6 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,963 + Pf1N1B4_2336 0.74 -0.0 2,653,964 - Pf1N1B4_2336 0.74 -2.2 2,653,964 - Pf1N1B4_2336 0.74 -2.8 2,654,048 + -0.0 2,654,049 - -1.3 2,654,049 - -2.5 2,654,052 + -1.7 2,654,052 + -0.0 2,654,053 - -0.0 2,654,064 + -0.0 2,654,064 + -0.0 2,654,165 + Pf1N1B4_2337 0.10 -1.0 2,654,174 - Pf1N1B4_2337 0.12 -0.0 2,654,180 + Pf1N1B4_2337 0.13 -4.2 2,654,180 + Pf1N1B4_2337 0.13 -4.0 2,654,180 + Pf1N1B4_2337 0.13 -1.6 2,654,180 + Pf1N1B4_2337 0.13 -0.0 2,654,181 - Pf1N1B4_2337 0.13 -6.5 2,654,181 - Pf1N1B4_2337 0.13 -0.0 2,654,307 + Pf1N1B4_2337 0.33 -4.9 2,654,307 + Pf1N1B4_2337 0.33 -0.0 2,654,307 + Pf1N1B4_2337 0.33 -0.0 2,654,308 - Pf1N1B4_2337 0.33 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + Pf1N1B4_2337 0.34 -0.0 2,654,316 + -0.0 2,654,317 - Pf1N1B4_2337 0.35 -2.8 2,654,317 - Pf1N1B4_2337 0.35 -3.8 2,654,451 + Pf1N1B4_2337 0.56 -0.0 2,654,739 + -0.0 2,654,739 + -0.0 2,654,741 + -0.0 2,654,741 + -4.4 2,654,742 - -0.0 2,654,752 + -5.1 2,654,752 + -1.0 2,654,752 + -0.0 2,654,752 + -0.0 2,654,752 + -4.6 2,654,752 + -3.8 2,654,752 + -0.0 2,654,752 + -5.3 2,654,752 + -5.3 2,654,753 - -0.0 2,654,753 - -0.0 2,654,773 + -0.0 2,654,773 + -4.8 2,654,773 + -0.0 2,654,773 + -0.0 2,654,993 - Pf1N1B4_2338 0.23 -4.1 2,654,993 - Pf1N1B4_2338 0.23 -0.0 2,654,993 - Pf1N1B4_2338 0.23 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -3.6 2,655,019 + Pf1N1B4_2338 0.25 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -0.0 2,655,019 + Pf1N1B4_2338 0.25 -3.7 2,655,020 - Pf1N1B4_2338 0.25 -0.0 2,655,020 - Pf1N1B4_2338 0.25 -6.0 2,655,062 - Pf1N1B4_2338 0.29 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,070 + Pf1N1B4_2338 0.30 -0.0 2,655,071 - Pf1N1B4_2338 0.30 -0.0 2,655,071 - Pf1N1B4_2338 0.30 -0.0 2,655,106 + Pf1N1B4_2338 0.33 -0.0 2,655,106 + Pf1N1B4_2338 0.33 -4.3 2,655,107 - Pf1N1B4_2338 0.33 -3.1 2,655,107 - Pf1N1B4_2338 0.33 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -4.6 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,169 + Pf1N1B4_2338 0.39 -0.0 2,655,170 - Pf1N1B4_2338 0.39 -3.2 2,655,170 - Pf1N1B4_2338 0.39 -4.8 2,655,170 - Pf1N1B4_2338 0.39 -0.0 2,655,170 - Pf1N1B4_2338 0.39 -0.0 2,655,569 + Pf1N1B4_2338 0.74 -0.0 2,655,569 + -0.0 2,655,569 + Pf1N1B4_2338 0.74 -1.0 2,655,570 - Pf1N1B4_2338 0.74 -5.4
Or see this region's nucleotide sequence