Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2278 and Pf1N1B4_2279 are separated by 108 nucleotides Pf1N1B4_2279 and Pf1N1B4_2280 are separated by 21 nucleotides Pf1N1B4_2280 and Pf1N1B4_2281 are separated by 39 nucleotides Pf1N1B4_2281 and Pf1N1B4_2282 are separated by 54 nucleotides
Pf1N1B4_2278 - Ribose 5-phosphate isomerase A (EC 5.3.1.6), at 2,594,127 to 2,594,801
_2278
Pf1N1B4_2279 - hypothetical protein, at 2,594,910 to 2,595,056
_2279
Pf1N1B4_2280 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 2,595,078 to 2,596,592
_2280
Pf1N1B4_2281 - Predicted integral membrane protein, at 2,596,632 to 2,597,123
_2281
Pf1N1B4_2282 - Phosphoserine phosphatase (EC 3.1.3.3), at 2,597,178 to 2,597,834
_2282
Position (kb)
2595
2596
2597 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2594.093 kb on + strand at 2594.093 kb on + strand at 2594.094 kb on - strand at 2594.109 kb on - strand at 2594.844 kb on - strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.911 kb on + strand at 2594.911 kb on + strand at 2594.917 kb on + strand at 2594.918 kb on - strand at 2594.918 kb on - strand at 2594.959 kb on + strand at 2594.959 kb on + strand, within Pf1N1B4_2279 at 2594.959 kb on + strand, within Pf1N1B4_2279 at 2594.959 kb on + strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.973 kb on - strand, within Pf1N1B4_2279 at 2594.973 kb on - strand, within Pf1N1B4_2279 at 2595.003 kb on + strand, within Pf1N1B4_2279 at 2595.004 kb on - strand, within Pf1N1B4_2279 at 2595.004 kb on - strand, within Pf1N1B4_2279 at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.121 kb on - strand at 2595.121 kb on - strand at 2595.121 kb on - strand at 2595.133 kb on + strand at 2595.186 kb on - strand at 2595.186 kb on - strand at 2595.243 kb on + strand at 2595.243 kb on + strand, within Pf1N1B4_2280 at 2595.244 kb on - strand, within Pf1N1B4_2280 at 2595.244 kb on - strand, within Pf1N1B4_2280 at 2595.245 kb on + strand, within Pf1N1B4_2280 at 2595.496 kb on - strand, within Pf1N1B4_2280 at 2595.501 kb on + strand, within Pf1N1B4_2280 at 2595.501 kb on + strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.513 kb on + strand, within Pf1N1B4_2280 at 2595.513 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.652 kb on - strand, within Pf1N1B4_2280 at 2595.652 kb on - strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.820 kb on - strand, within Pf1N1B4_2280 at 2595.820 kb on - strand, within Pf1N1B4_2280 at 2595.824 kb on - strand, within Pf1N1B4_2280 at 2595.876 kb on - strand, within Pf1N1B4_2280 at 2595.879 kb on + strand, within Pf1N1B4_2280 at 2595.880 kb on - strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2596.152 kb on + strand, within Pf1N1B4_2280 at 2596.152 kb on + strand, within Pf1N1B4_2280 at 2596.159 kb on - strand, within Pf1N1B4_2280 at 2596.310 kb on + strand, within Pf1N1B4_2280 at 2596.310 kb on + strand, within Pf1N1B4_2280 at 2596.471 kb on + strand at 2596.471 kb on + strand at 2596.546 kb on - strand at 2596.546 kb on - strand at 2596.573 kb on - strand at 2596.573 kb on - strand at 2596.665 kb on - strand at 2596.665 kb on - strand at 2596.665 kb on - strand at 2596.760 kb on + strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.900 kb on - strand, within Pf1N1B4_2281 at 2596.900 kb on - strand, within Pf1N1B4_2281 at 2596.982 kb on + strand, within Pf1N1B4_2281 at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.125 kb on + strand at 2597.125 kb on + strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,594,093 + -1.7 2,594,093 + -1.8 2,594,094 - -0.0 2,594,109 - -0.8 2,594,844 - -2.6 2,594,845 + -0.0 2,594,845 + -0.0 2,594,845 + -0.0 2,594,845 + -0.0 2,594,845 + -0.0 2,594,845 + -0.0 2,594,846 - -0.0 2,594,846 - -0.0 2,594,846 - -2.6 2,594,846 - -2.2 2,594,846 - -0.0 2,594,911 + -2.3 2,594,911 + -0.0 2,594,917 + -0.6 2,594,918 - -0.9 2,594,918 - -0.0 2,594,959 + -0.0 2,594,959 + Pf1N1B4_2279 0.33 -1.9 2,594,959 + Pf1N1B4_2279 0.33 -0.0 2,594,959 + Pf1N1B4_2279 0.33 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -3.0 2,594,960 - Pf1N1B4_2279 0.34 -0.0 2,594,960 - Pf1N1B4_2279 0.34 -1.6 2,594,963 + Pf1N1B4_2279 0.36 -0.0 2,594,963 + Pf1N1B4_2279 0.36 -0.0 2,594,963 + Pf1N1B4_2279 0.36 -0.0 2,594,963 + Pf1N1B4_2279 0.36 -0.0 2,594,963 + Pf1N1B4_2279 0.36 -0.0 2,594,964 - Pf1N1B4_2279 0.37 -0.0 2,594,964 - Pf1N1B4_2279 0.37 -0.0 2,594,964 - Pf1N1B4_2279 0.37 -0.0 2,594,964 - Pf1N1B4_2279 0.37 -0.0 2,594,973 - Pf1N1B4_2279 0.43 -4.4 2,594,973 - Pf1N1B4_2279 0.43 -2.0 2,595,003 + Pf1N1B4_2279 0.63 -0.0 2,595,004 - Pf1N1B4_2279 0.64 -0.0 2,595,004 - Pf1N1B4_2279 0.64 -0.0 2,595,090 + -0.0 2,595,090 + -0.0 2,595,090 + -0.0 2,595,090 + -0.0 2,595,090 + -0.0 2,595,091 - -4.6 2,595,091 - -0.0 2,595,091 - -3.7 2,595,091 - -3.7 2,595,091 - -0.0 2,595,091 - -4.4 2,595,091 - -2.1 2,595,121 - -0.0 2,595,121 - -0.0 2,595,121 - -0.0 2,595,133 + -5.6 2,595,186 - -3.6 2,595,186 - -1.8 2,595,243 + -4.8 2,595,243 + Pf1N1B4_2280 0.11 -4.0 2,595,244 - Pf1N1B4_2280 0.11 -3.0 2,595,244 - Pf1N1B4_2280 0.11 -0.0 2,595,245 + Pf1N1B4_2280 0.11 -6.4 2,595,496 - Pf1N1B4_2280 0.28 -0.0 2,595,501 + Pf1N1B4_2280 0.28 -0.0 2,595,501 + Pf1N1B4_2280 0.28 -3.3 2,595,502 - Pf1N1B4_2280 0.28 -0.0 2,595,502 - Pf1N1B4_2280 0.28 -0.0 2,595,502 - Pf1N1B4_2280 0.28 -0.0 2,595,502 - Pf1N1B4_2280 0.28 -3.5 2,595,513 + Pf1N1B4_2280 0.29 -0.0 2,595,513 + Pf1N1B4_2280 0.29 -1.3 2,595,651 + Pf1N1B4_2280 0.38 -4.1 2,595,651 + Pf1N1B4_2280 0.38 -0.0 2,595,651 + Pf1N1B4_2280 0.38 -0.0 2,595,651 + Pf1N1B4_2280 0.38 -0.0 2,595,652 - Pf1N1B4_2280 0.38 -3.4 2,595,652 - Pf1N1B4_2280 0.38 -0.0 2,595,819 + Pf1N1B4_2280 0.49 -4.0 2,595,819 + Pf1N1B4_2280 0.49 -4.2 2,595,819 + Pf1N1B4_2280 0.49 -0.0 2,595,820 - Pf1N1B4_2280 0.49 -0.0 2,595,820 - Pf1N1B4_2280 0.49 -3.3 2,595,824 - Pf1N1B4_2280 0.49 -0.0 2,595,876 - Pf1N1B4_2280 0.53 -4.0 2,595,879 + Pf1N1B4_2280 0.53 -3.9 2,595,880 - Pf1N1B4_2280 0.53 +1.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -5.1 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -0.0 2,595,942 + Pf1N1B4_2280 0.57 -4.9 2,595,942 + Pf1N1B4_2280 0.57 -3.8 2,595,942 + Pf1N1B4_2280 0.57 -4.4 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,595,943 - -0.0 2,595,943 - Pf1N1B4_2280 0.57 -0.0 2,596,152 + Pf1N1B4_2280 0.71 -0.0 2,596,152 + Pf1N1B4_2280 0.71 -3.6 2,596,159 - Pf1N1B4_2280 0.71 -0.0 2,596,310 + Pf1N1B4_2280 0.81 -0.0 2,596,310 + Pf1N1B4_2280 0.81 -0.0 2,596,471 + -0.0 2,596,471 + -5.2 2,596,546 - -0.0 2,596,546 - -0.0 2,596,573 - -4.2 2,596,573 - -4.3 2,596,665 - -0.0 2,596,665 - -1.6 2,596,665 - -0.0 2,596,760 + Pf1N1B4_2281 0.26 -0.0 2,596,761 - Pf1N1B4_2281 0.26 -0.0 2,596,761 - Pf1N1B4_2281 0.26 -2.4 2,596,761 - Pf1N1B4_2281 0.26 -2.3 2,596,761 - Pf1N1B4_2281 0.26 -0.0 2,596,900 - Pf1N1B4_2281 0.54 -0.0 2,596,900 - Pf1N1B4_2281 0.54 -2.1 2,596,982 + Pf1N1B4_2281 0.71 -0.0 2,597,121 + -2.9 2,597,121 + -0.0 2,597,121 + +1.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -1.8 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -1.2 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -3.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -1.5 2,597,121 + -2.7 2,597,121 + -2.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -0.0 2,597,121 + -2.9 2,597,121 + -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -4.5 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -3.0 2,597,122 - -0.0 2,597,122 - -0.4 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -1.0 2,597,122 - -1.1 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,122 - -0.0 2,597,125 + -1.1 2,597,125 + -0.0 2,597,126 - -0.0 2,597,126 - -2.3 2,597,126 - -1.2 2,597,126 - -0.0 2,597,126 - -0.0 2,597,126 - -0.0 2,597,126 - -0.0 2,597,126 - -3.2 2,597,305 + Pf1N1B4_2282 0.19 -0.0 2,597,305 + Pf1N1B4_2282 0.19 -3.4 2,597,305 + Pf1N1B4_2282 0.19 -0.0 2,597,306 - Pf1N1B4_2282 0.19 -0.0 2,597,306 - Pf1N1B4_2282 0.19 -0.0 2,597,306 - Pf1N1B4_2282 0.19 -4.3 2,597,306 - Pf1N1B4_2282 0.19 -4.2 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -4.7 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,311 + Pf1N1B4_2282 0.20 -3.9 2,597,311 + Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -3.7 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -0.0 2,597,312 - Pf1N1B4_2282 0.20 -4.3 2,597,312 - Pf1N1B4_2282 0.20 -6.2 2,597,312 - Pf1N1B4_2282 0.20 -2.6 2,597,312 - Pf1N1B4_2282 0.20 -3.0
Or see this region's nucleotide sequence